Restriction map for
pF1KB6586
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BciVI BglII BlnI BsaHI BsaI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI StuI SwaI TfiI
Tth111I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 696 GGTACC G^GTACC
AccI 415 GTAGAC GT^[AC][GT]AC
AccII 712 CGCG CG^CG
AcsI 913 AAATTC [GA]^AATT[CT]
Alw44I 1042 GTGCAC G^TGCAC
AlwNI 268 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 40 CCTCAGG CC^T[ACGT]AGG
ApaLI 1042 GTGCAC G^TGCAC
ApoI 913 AAATTC [GA]^AATT[CT]
Asp718I 696 GGTACC G^GTACC
AspHI 1042 GTGCAC G[AT]GC[AT]^C
BalI 227 TGGCCA TGG^CCA
BanII 927 GGGCTC G[GA]GC[CT]^C
BbvCI 858 GCTGAGG CCTCAGC(-5/-2)
BclI 590 TGATCA T^GATCA
BfaI 1144 CTAG C^TAG
BglI 112 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 378 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 378 GCTGAGC GC^T[ACGT]AGC
BsaAI 670 TACGTG [CT]AC^GT[GA]
BsaWI 740 ACCGGA [AT]^CCGG[AT]
BseRI 13 CTCCTC GAGGAG(10/8)
BsgI 319 CTGCAC GTGCAG(16/14)
BsiEI 584 CGGTCG CG[GA][CT]^CG
BsiHKAI 1042 GTGCAC G[AT]GC[AT]^C
BstF5I 1188 CATCC GGATG(2/0)
BstUI 712 CGCG CG^CG
BstXI 218 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 269 AGATCC [GA]^GATC[CT]
Bsu36I 40 CCTCAGG CC^T[ACGT]AGG
Cac8I 1123 GCCCGC GC[ACGT]^[ACGT]GC
CelII 378 GCTGAGC GC^T[ACGT]AGC
DraI 613 TTTAAA TTT^AAA
EaeI 227 TGGCCA [CT]^GGCC[GA]
Eco81I 40 CCTCAGG CC^T[ACGT]AGG
FbaI 590 TGATCA T^GATCA
FokI 1188 CATCC GGATG(9/13)
HgaI 450 GACGC GACGC(5/10)
HindIII 633 AAGCTT A^AGCTT
KpnI 696 GGTACC GGTAC^C
MaeI 1144 CTAG C^TAG
MflI 269 AGATCC [GA]^GATC[CT]
MluNI 227 TGGCCA TGG^CCA
MscI 227 TGGCCA TGG^CCA
MvnI 712 CGCG CG^CG
PaeR7I 895 CTCGAG C^TCGAG
SapI 6 GCTCTTC GCTCTTC(1/4)
SexAI 407 ACCTGGT A^CC[AT]GGT
SmaI 71 CCCGGG CCC^GGG
SspI 1104 AATATT AAT^ATT
Tsp45I 673 GTGAC ^GT[GC]AC
TspRI 810 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 895 CTCGAG C^TCGAG
XhoII 269 AGATCC [GA]^GATC[CT]
XmaCI 71 CCCGGG C^CCGGG
XmaI 71 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 456 GTAC GT^AC
697 GTAC
AflII 562 CTTAAG C^TTAAG
871 CTTAAG
AvaI 71 CCCGGG C^[CT]CG[GA]G
895 CTCGAG
BbsI 447 GAAGAC GAAGAC(2/6)
976 GTCTTC
BcgI 573 CGATAGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
578 GCAATGCGGTCG
BcnI 71 CCCGG CC^[GC]GG
72 CCGGG
BfrI 562 CTTAAG C^TTAAG
871 CTTAAG
BmyI 927 GGGCTC G[AGT]GC[ACT]^C
1042 GTGCAC
BpuAI 447 GAAGAC GAAGAC(2/6)
976 GTCTTC
BsaBI 264 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
718 GATCAGTATC
BsmAI 628 GAGAC GTCTC(1/5)
682 GAGAC
BsoBI 71 CCCGGG C^[CT]CG[GA]G
895 CTCGAG
Bsp1286I 927 GGGCTC G[AGT]GC[ACT]^C
1042 GTGCAC
BspMI 419 ACCTGC ACCTGC(4/8)
828 GCAGGT
BsrI 809 CCAGT ACTGG(1/-1)
956 CCAGT
FauI 1124 CCCGC CCCGC(4/6)
693 GCGGG
MaeII 671 ACGT A^CGT
974 ACGT
MaeIII 673 GTGAC ^GT[ACGT]AC
971 GTAAC
MamI 264 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
718 GATCAGTATC
NciI 71 CCCGG CC^[GC]GG
72 CCGGG
PpuMI 95 AGGACCT [GA]G^G[AT]CC[CT]
767 GGGTCCC
PvuII 376 CAGCTG CAG^CTG
937 CAGCTG
RsaI 456 GTAC GT^AC
697 GTAC
SbfI 420 CCTGCAGG CCTGCA^GG
752 CCTGCAGG
Sse8387I 420 CCTGCAGG CCTGCA^GG
752 CCTGCAGG
Tsp509I 601 AATT ^AATT
914 AATT
XcmI 230 CCATGCTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
357 CCAGCCCAGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvI BpmI BsaJI BsiYI BslI
BsmFI BsrDI BssKI BstNI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
TthHB8I