################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII AccII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AspI BbrPI BbsI BciVI BclI BfrI BglII BpuAI BsaAI BsiWI BsmI Bsp1407I BspDI BspHI BsrBI BsrGI BssHII BstEII BstPI BstUI ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MfeI MluI MseI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AflIII 2125 ACATGT A^C[GA][CT]GT Alw44I 2185 GTGCAC G^TGCAC ApaLI 2185 GTGCAC G^TGCAC AviII 1913 TGCGCA TGC^GCA AvrII 495 CCTAGG C^CTAGG BbeI 1171 GGCGCC GGCGC^C BcgI 1768 CGAGCCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 495 CCTAGG C^CTAGG BlpI 1494 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1494 GCTCAGC GC^T[ACGT]AGC BsaBI 615 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspLU11I 2125 ACATGT A^CATGT BssSI 2592 CTCGTG CACGAG(-5/-1) Bst1107I 2830 GTATAC GTA^TAC BstAPI 2740 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 446 TTCGAA TT^CGAA BstZ17I 2830 GTATAC GTA^TAC CelII 1494 GCTCAGC GC^T[ACGT]AGC DrdI 413 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1275 CGGCCG C^GGCCG EclXI 1275 CGGCCG C^GGCCG Eco52I 1275 CGGCCG C^GGCCG EcoRI 223 GAATTC G^AATTC FspI 1913 TGCGCA TGC^GCA HincII 1723 GTTGAC GT[CT]^[GA]AC HindII 1723 GTTGAC GT[CT]^[GA]AC KasI 1171 GGCGCC G^GCGCC MaeII 1433 ACGT A^CGT MamI 615 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1171 GGCGCC GG^CGCC NspV 446 TTCGAA TT^CGAA PshAI 2122 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1522 GAGCTC GAGCT^C SapI 2160 GAAGAGC GCTCTTC(1/4) SexAI 2225 ACCAGGT A^CC[AT]GGT SfoI 1171 GGCGCC GGC^GCC SfuI 446 TTCGAA TT^CGAA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 384 GGTACC G^GTACC 1654 GGTACC AccI 829 GTCTAC GT^[AC][GT]AC 2830 GTATAC AccIII 423 TCCGGA T^CCGGA 2130 TCCGGA AcsI 223 GAATTC [GA]^AATT[CT] 738 GAATTT AlwNI 534 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 890 CAGTGACTG AocI 993 CCTGAGG CC^T[ACGT]AGG 1348 CCTGAGG ApoI 223 GAATTC [GA]^AATT[CT] 738 GAATTT Asp718I 384 GGTACC G^GTACC 1654 GGTACC BamHI 620 GGATCC G^GATCC 2236 GGATCC BbvCI 780 CCTCAGC CCTCAGC(-5/-2) 261 GCTGAGG BfaI 496 CTAG C^TAG 2182 CTAG BglI 749 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2447 GCCAGGCTGGC BsaI 667 GAGACC GGTCTC(1/5) 2518 GGTCTC BsaWI 423 TCCGGA [AT]^CCGG[AT] 2130 TCCGGA BseAI 423 TCCGGA T^CCGGA 2130 TCCGGA BsiEI 1275 CGGCCG CG[GA][CT]^CG 1839 CGGTCG BsmBI 1284 CGTCTC CGTCTC(1/5) 2969 GAGACG BspEI 423 TCCGGA T^CCGGA 2130 TCCGGA BsrFI 1017 ACCGGC [GA]^CCGG[CT] 1273 ACCGGC BstXI 2313 CCACGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2753 CCAGCACCCTGG Bsu36I 993 CCTGAGG CC^T[ACGT]AGG 1348 CCTGAGG Cfr10I 1017 ACCGGC [GA]^CCGG[CT] 1273 ACCGGC EarI 1876 GAAGAG CTCTTC(1/4) 2160 GAAGAG Eco81I 993 CCTGAGG CC^T[ACGT]AGG 1348 CCTGAGG HaeII 39 GGCGCT [GA]GCGC^[CT] 1171 GGCGCC HgaI 2342 GACGC GACGC(5/10) 161 GCGTC KpnI 384 GGTACC GGTAC^C 1654 GGTACC Ksp632I 1876 GAAGAG CTCTTC(1/4) 2160 GAAGAG MaeI 496 CTAG C^TAG 2182 CTAG MroI 423 TCCGGA T^CCGGA 2130 TCCGGA NspI 1869 GCATGT [GA]CATG^[CT] 2125 ACATGT PstI 1550 CTGCAG CTGCA^G 2869 CTGCAG ScaI 1528 AGTACT AGT^ACT 1982 AGTACT StuI 903 AGGCCT AGG^CCT 1574 AGGCCT Tsp509I 224 AATT ^AATT 739 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI