Restriction map for
pF1KB7331
##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI BbrPI BbsI
BciVI BclI BfrI BglII BpuAI
BsaAI BsiWI BsmI Bsp1407I BspDI
BspHI BsrBI BsrGI BssHII BstEII
BstPI BstUI ClaI CpoI DraI
DraIII Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FbaI FseI HindIII HpaI
KspI MfeI MluI MseI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 2125 ACATGT A^C[GA][CT]GT
Alw44I 2185 GTGCAC G^TGCAC
ApaLI 2185 GTGCAC G^TGCAC
AviII 1913 TGCGCA TGC^GCA
AvrII 495 CCTAGG C^CTAGG
BbeI 1171 GGCGCC GGCGC^C
BcgI 1768 CGAGCCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 495 CCTAGG C^CTAGG
BlpI 1494 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1494 GCTCAGC GC^T[ACGT]AGC
BsaBI 615 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 2125 ACATGT A^CATGT
BssSI 2592 CTCGTG CACGAG(-5/-1)
Bst1107I 2830 GTATAC GTA^TAC
BstAPI 2740 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 446 TTCGAA TT^CGAA
BstZ17I 2830 GTATAC GTA^TAC
CelII 1494 GCTCAGC GC^T[ACGT]AGC
DrdI 413 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1275 CGGCCG C^GGCCG
EclXI 1275 CGGCCG C^GGCCG
Eco52I 1275 CGGCCG C^GGCCG
EcoRI 223 GAATTC G^AATTC
FspI 1913 TGCGCA TGC^GCA
HincII 1723 GTTGAC GT[CT]^[GA]AC
HindII 1723 GTTGAC GT[CT]^[GA]AC
KasI 1171 GGCGCC G^GCGCC
MaeII 1433 ACGT A^CGT
MamI 615 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1171 GGCGCC GG^CGCC
NspV 446 TTCGAA TT^CGAA
PshAI 2122 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1522 GAGCTC GAGCT^C
SapI 2160 GAAGAGC GCTCTTC(1/4)
SexAI 2225 ACCAGGT A^CC[AT]GGT
SfoI 1171 GGCGCC GGC^GCC
SfuI 446 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 384 GGTACC G^GTACC
1654 GGTACC
AccI 829 GTCTAC GT^[AC][GT]AC
2830 GTATAC
AccIII 423 TCCGGA T^CCGGA
2130 TCCGGA
AcsI 223 GAATTC [GA]^AATT[CT]
738 GAATTT
AlwNI 534 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
890 CAGTGACTG
AocI 993 CCTGAGG CC^T[ACGT]AGG
1348 CCTGAGG
ApoI 223 GAATTC [GA]^AATT[CT]
738 GAATTT
Asp718I 384 GGTACC G^GTACC
1654 GGTACC
BamHI 620 GGATCC G^GATCC
2236 GGATCC
BbvCI 780 CCTCAGC CCTCAGC(-5/-2)
261 GCTGAGG
BfaI 496 CTAG C^TAG
2182 CTAG
BglI 749 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2447 GCCAGGCTGGC
BsaI 667 GAGACC GGTCTC(1/5)
2518 GGTCTC
BsaWI 423 TCCGGA [AT]^CCGG[AT]
2130 TCCGGA
BseAI 423 TCCGGA T^CCGGA
2130 TCCGGA
BsiEI 1275 CGGCCG CG[GA][CT]^CG
1839 CGGTCG
BsmBI 1284 CGTCTC CGTCTC(1/5)
2969 GAGACG
BspEI 423 TCCGGA T^CCGGA
2130 TCCGGA
BsrFI 1017 ACCGGC [GA]^CCGG[CT]
1273 ACCGGC
BstXI 2313 CCACGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2753 CCAGCACCCTGG
Bsu36I 993 CCTGAGG CC^T[ACGT]AGG
1348 CCTGAGG
Cfr10I 1017 ACCGGC [GA]^CCGG[CT]
1273 ACCGGC
EarI 1876 GAAGAG CTCTTC(1/4)
2160 GAAGAG
Eco81I 993 CCTGAGG CC^T[ACGT]AGG
1348 CCTGAGG
HaeII 39 GGCGCT [GA]GCGC^[CT]
1171 GGCGCC
HgaI 2342 GACGC GACGC(5/10)
161 GCGTC
KpnI 384 GGTACC GGTAC^C
1654 GGTACC
Ksp632I 1876 GAAGAG CTCTTC(1/4)
2160 GAAGAG
MaeI 496 CTAG C^TAG
2182 CTAG
MroI 423 TCCGGA T^CCGGA
2130 TCCGGA
NspI 1869 GCATGT [GA]CATG^[CT]
2125 ACATGT
PstI 1550 CTGCAG CTGCA^G
2869 CTGCAG
ScaI 1528 AGTACT AGT^ACT
1982 AGTACT
StuI 903 AGGCCT AGG^CCT
1574 AGGCCT
Tsp509I 224 AATT ^AATT
739 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI