Restriction map for pF1KB7331
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       AccII       AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp700I     AspEI       AspI        BbrPI       BbsI        
BciVI       BclI        BfrI        BglII       BpuAI       
BsaAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspHI       BsrBI       BsrGI       BssHII      BstEII      
BstPI       BstUI       ClaI        CpoI        DraI        
DraIII      Eam1105I    Eco47III    EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KspI        MfeI        MluI        MseI        MunI        
MvnI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SfiI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AflIII       2125  ACATGT               A^C[GA][CT]GT
Alw44I       2185  GTGCAC               G^TGCAC
ApaLI        2185  GTGCAC               G^TGCAC
AviII        1913  TGCGCA               TGC^GCA
AvrII         495  CCTAGG               C^CTAGG
BbeI         1171  GGCGCC               GGCGC^C
BcgI         1768  CGAGCCGTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI          495  CCTAGG               C^CTAGG
BlpI         1494  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1494  GCTCAGC              GC^T[ACGT]AGC
BsaBI         615  GATCGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I     2125  ACATGT               A^CATGT
BssSI        2592  CTCGTG               CACGAG(-5/-1)
Bst1107I     2830  GTATAC               GTA^TAC
BstAPI       2740  GCACCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         446  TTCGAA               TT^CGAA
BstZ17I      2830  GTATAC               GTA^TAC
CelII        1494  GCTCAGC              GC^T[ACGT]AGC
DrdI          413  GACCCTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1275  CGGCCG               C^GGCCG
EclXI        1275  CGGCCG               C^GGCCG
Eco52I       1275  CGGCCG               C^GGCCG
EcoRI         223  GAATTC               G^AATTC
FspI         1913  TGCGCA               TGC^GCA
HincII       1723  GTTGAC               GT[CT]^[GA]AC
HindII       1723  GTTGAC               GT[CT]^[GA]AC
KasI         1171  GGCGCC               G^GCGCC
MaeII        1433  ACGT                 A^CGT
MamI          615  GATCGGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         1171  GGCGCC               GG^CGCC
NspV          446  TTCGAA               TT^CGAA
PshAI        2122  GACACATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1522  GAGCTC               GAGCT^C
SapI         2160  GAAGAGC              GCTCTTC(1/4)
SexAI        2225  ACCAGGT              A^CC[AT]GGT
SfoI         1171  GGCGCC               GGC^GCC
SfuI          446  TTCGAA               TT^CGAA


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

Acc65I        384  GGTACC               G^GTACC
             1654  GGTACC              
AccI          829  GTCTAC               GT^[AC][GT]AC
             2830  GTATAC              
AccIII        423  TCCGGA               T^CCGGA
             2130  TCCGGA              
AcsI          223  GAATTC               [GA]^AATT[CT]
              738  GAATTT              
AlwNI         534  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              890  CAGTGACTG           
AocI          993  CCTGAGG              CC^T[ACGT]AGG
             1348  CCTGAGG             
ApoI          223  GAATTC               [GA]^AATT[CT]
              738  GAATTT              
Asp718I       384  GGTACC               G^GTACC
             1654  GGTACC              
BamHI         620  GGATCC               G^GATCC
             2236  GGATCC              
BbvCI         780  CCTCAGC              CCTCAGC(-5/-2)
              261  GCTGAGG             
BfaI          496  CTAG                 C^TAG
             2182  CTAG                
BglI          749  GCCCAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2447  GCCAGGCTGGC         
BsaI          667  GAGACC               GGTCTC(1/5)
             2518  GGTCTC              
BsaWI         423  TCCGGA               [AT]^CCGG[AT]
             2130  TCCGGA              
BseAI         423  TCCGGA               T^CCGGA
             2130  TCCGGA              
BsiEI        1275  CGGCCG               CG[GA][CT]^CG
             1839  CGGTCG              
BsmBI        1284  CGTCTC               CGTCTC(1/5)
             2969  GAGACG              
BspEI         423  TCCGGA               T^CCGGA
             2130  TCCGGA              
BsrFI        1017  ACCGGC               [GA]^CCGG[CT]
             1273  ACCGGC              
BstXI        2313  CCACGATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2753  CCAGCACCCTGG        
Bsu36I        993  CCTGAGG              CC^T[ACGT]AGG
             1348  CCTGAGG             
Cfr10I       1017  ACCGGC               [GA]^CCGG[CT]
             1273  ACCGGC              
EarI         1876  GAAGAG               CTCTTC(1/4)
             2160  GAAGAG              
Eco81I        993  CCTGAGG              CC^T[ACGT]AGG
             1348  CCTGAGG             
HaeII          39  GGCGCT               [GA]GCGC^[CT]
             1171  GGCGCC              
HgaI         2342  GACGC                GACGC(5/10)
              161  GCGTC               
KpnI          384  GGTACC               GGTAC^C
             1654  GGTACC              
Ksp632I      1876  GAAGAG               CTCTTC(1/4)
             2160  GAAGAG              
MaeI          496  CTAG                 C^TAG
             2182  CTAG                
MroI          423  TCCGGA               T^CCGGA
             2130  TCCGGA              
NspI         1869  GCATGT               [GA]CATG^[CT]
             2125  ACATGT              
PstI         1550  CTGCAG               CTGCA^G
             2869  CTGCAG              
ScaI         1528  AGTACT               AGT^ACT
             1982  AGTACT              
StuI          903  AGGCCT               AGG^CCT
             1574  AGGCCT              
Tsp509I       224  AATT                 ^AATT
              739  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI        
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