Restriction map for
pF1KE0211
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# NO CUT ENZYMES ( 213 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DpnII
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII ItaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluI MluNI MroI
MscI MseI MslI MspI MunI
MvaI MwoI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AluI 29 AGCT AG^CT
AspHI 34 GAGCAC G[AT]GC[AT]^C
AvaI 247 CCCGAG C^[CT]CG[GA]G
AvaII 229 GGACC G^G[AT]CC
BmyI 34 GAGCAC G[AGT]GC[ACT]^C
BpmI 31 CTGGAG CTGGAG(16/14)
BsaJI 248 CCGAGG C^C[ACGT][ACGT]GG
BseRI 250 GAGGAG GAGGAG(10/8)
BsiHKAI 34 GAGCAC G[AT]GC[AT]^C
BsiYI 77 CCACCAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 77 CCACCAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 247 CCCGAG C^[CT]CG[GA]G
Bsp1286I 34 GAGCAC G[AGT]GC[ACT]^C
BspMI 38 ACCTGC ACCTGC(4/8)
BsrDI 162 CATTGC GCAATG(2/0)
BstF5I 18 CATCC GGATG(2/0)
CfoI 184 GCGC GCG^C
DraIII 78 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
FokI 18 CATCC GGATG(9/13)
HhaI 184 GCGC GCG^C
HinP1I 184 GCGC G^CGC
HphI 96 GGTGA GGTGA(8/7)
MnlI 250 GAGG CCTC(7/6)
MspA1I 28 CAGCTG C[AC]G^C[GT]G
NlaIII 129 CATG CATG^
PflMI 77 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 40 CTGCAG CTGCA^G
PvuII 28 CAGCTG CAG^CTG
SfaNI 213 GATGC GCATC(5/9)
SfcI 40 CTGCAG C^T[GA][CT]AG
TaqI 140 TCGA T^CGA
TthHB8I 140 TCGA T^CGA
Van91I 77 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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AccII 109 CGCG CG^CG
185 CGCG
AfaI 51 GTAC GT^AC
117 GTAC
BstUI 109 CGCG CG^CG
185 CGCG
Cac8I 26 GCCAGC GC[ACGT]^[ACGT]GC
234 GCCAGC
HaeIII 64 GGCC GG^CC
133 GGCC
HinfI 112 GACTC G^A[ACGT]TC
188 GAGTC
MvnI 109 CGCG CG^CG
185 CGCG
PleI 112 GACTC GAGTC(4/5)
188 GAGTC
RsaI 51 GTAC GT^AC
117 GTAC
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
AciI Cfr13I Sau96I