Restriction map for
pF1KB6462
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BlnI BpuAI BsaAI
BsaBI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I ClaI
CpoI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FspI
HindIII HpaI KasI KpnI KspI
MaeII MamI MluI MvnI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TspRI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AccI 488 GTCGAC GT^[AC][GT]AC
AccIII 534 TCCGGA T^CCGGA
AciI 363 GCGG CCGC(-3/-1)
AcyI 491 GACGCC G[GA]^CG[CT]C
AlwNI 691 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 259 CCTCAGG CC^T[ACGT]AGG
AspHI 887 GAGCTC G[AT]GC[AT]^C
BanII 887 GAGCTC G[GA]GC[CT]^C
BclI 209 TGATCA T^GATCA
BglI 1001 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 447 AGATCT A^GATCT
BmyI 887 GAGCTC G[AGT]GC[ACT]^C
BsaHI 491 GACGCC G[GA]^CG[CT]C
BsaI 826 GGTCTC GGTCTC(1/5)
BsaJI 231 CCGTGG C^C[ACGT][ACGT]GG
BsaWI 534 TCCGGA [AT]^CCGG[AT]
BseAI 534 TCCGGA T^CCGGA
BseRI 65 GAGGAG GAGGAG(10/8)
BsgI 647 CTGCAC GTGCAG(16/14)
BsiHKAI 887 GAGCTC G[AT]GC[AT]^C
Bsp1286I 887 GAGCTC G[AGT]GC[ACT]^C
BspEI 534 TCCGGA T^CCGGA
BspHI 438 TCATGA T^CATGA
BsrFI 10 GCCGGC [GA]^CCGG[CT]
Bsu36I 259 CCTCAGG CC^T[ACGT]AGG
CfoI 271 GCGC GCG^C
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
DraI 931 TTTAAA TTT^AAA
DraIII 338 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 231 CCGTGG C^C[GA][CT]GG
Eco81I 259 CCTCAGG CC^T[ACGT]AGG
EcoRV 943 GATATC GAT^ATC
FauI 363 GCGGG CCCGC(4/6)
FbaI 209 TGATCA T^GATCA
FseI 9 GGCCGGCC GGCCGG^CC
HaeII 270 GGCGCT [GA]GCGC^[CT]
HgaI 491 GACGC GACGC(5/10)
HhaI 271 GCGC GCG^C
Hin1I 491 GACGCC G[GA]^CG[CT]C
HinP1I 271 GCGC G^CGC
HincII 488 GTCGAC GT[CT]^[GA]AC
HindII 488 GTCGAC GT[CT]^[GA]AC
MfeI 161 CAATTG C^AATTG
MroI 534 TCCGGA T^CCGGA
MseI 932 TTAA T^TAA
MslI 396 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 664 CAGCTG C[AC]G^C[GT]G
MunI 161 CAATTG C^AATTG
NaeI 10 GCCGGC GCC^GGC
NgoMIV 10 GCCGGC G^CCGGC
NspI 599 GCATGT [GA]CATG^[CT]
PflMI 334 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 697 CTGCAG CTGCA^G
PvuII 664 CAGCTG CAG^CTG
RcaI 438 TCATGA T^CATGA
SacI 887 GAGCTC GAGCT^C
SalI 488 GTCGAC G^TCGAC
SapI 541 GAAGAGC GCTCTTC(1/4)
ScaI 808 AGTACT AGT^ACT
SfaNI 990 GATGC GCATC(5/9)
SfcI 697 CTGCAG C^T[GA][CT]AG
SmlI 16 CTCAAG C^T[CT][GA]AG
TaqI 489 TCGA T^CGA
Tru9I 932 TTAA T^TAA
TthHB8I 489 TCGA T^CGA
Van91I 334 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 558 TCTAGA T^CTAGA
XcmI 1019 CCATCAATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 693 GATCC GGATC(4/5)
870 GGATC
BalI 234 TGGCCA TGG^CCA
999 TGGCCA
BsmAI 803 GAGAC GTCTC(1/5)
827 GTCTC
BstYI 447 AGATCT [GA]^GATC[CT]
692 AGATCC
Cfr13I 247 GGGCC G^G[ACGT]CC
331 GGCCC
HapII 11 CCGG C^CGG
535 CCGG
HpaII 11 CCGG C^CGG
535 CCGG
HphI 343 GGTGA GGTGA(8/7)
125 TCACC
MaeIII 124 GTCAC ^GT[ACGT]AC
706 GTCAC
MflI 447 AGATCT [GA]^GATC[CT]
692 AGATCC
MluNI 234 TGGCCA TGG^CCA
999 TGGCCA
MscI 234 TGGCCA TGG^CCA
999 TGGCCA
MspI 11 CCGG C^CGG
535 CCGG
NlaIV 76 GGAACC GG[ACGT]^[ACGT]CC
246 GGGGCC
Sau96I 247 GGGCC G^G[ACGT]CC
331 GGCCC
TfiI 432 GAATC G^A[AT]TC
531 GATTC
Tsp45I 124 GTCAC ^GT[GC]AC
706 GTCAC
XhoII 447 AGATCT [GA]^GATC[CT]
692 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbvI BfaI BlpI BpmI Bpu1102I
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I CelII DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII PleI RsaI Sau3AI
ScrFI TseI Tsp509I XmnI