Restriction map for
pF1KB9760
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI ApoI AseI
AsnI Asp700I AspEI AspI BbrPI
BbsI BclI BfrI BglII BlpI
Bpu1102I BpuAI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstZ17I CelII ClaI DraI DraIII
DrdI Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SalI SapI ScaI SexAI
SfaNI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tru9I Tsp509I
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1359 GGTACC G^GTACC
AccI 613 GTCTAC GT^[AC][GT]AC
Alw44I 1546 GTGCAC G^TGCAC
ApaLI 1546 GTGCAC G^TGCAC
AscI 787 GGCGCGCC GG^CGCGCC
Asp718I 1359 GGTACC G^GTACC
AviII 563 TGCGCA TGC^GCA
AvrII 1839 CCTAGG C^CTAGG
BamHI 193 GGATCC G^GATCC
BbeI 1944 GGCGCC GGCGC^C
BciVI 1383 GTATCC GTATCC(6/5)
BfaI 1840 CTAG C^TAG
BlnI 1839 CCTAGG C^CTAGG
BsaAI 359 TACGTG [CT]AC^GT[GA]
BsaBI 811 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 767 ACCGGA [AT]^CCGG[AT]
BstEII 1452 GGTGACC G^GT[ACGT]ACC
BstPI 1452 GGTGACC G^GT[ACGT]ACC
BstXI 908 CCATATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 193 GGATCC [GA]^GATC[CT]
CpoI 769 CGGACCG CG^G[AT]CCG
EarI 76 GAAGAG CTCTTC(1/4)
EcoO65I 1452 GGTGACC G^GT[ACGT]ACC
FspI 563 TGCGCA TGC^GCA
HincII 2140 GTTGAC GT[CT]^[GA]AC
HindII 2140 GTTGAC GT[CT]^[GA]AC
KasI 1944 GGCGCC G^GCGCC
KpnI 1359 GGTACC GGTAC^C
Ksp632I 76 GAAGAG CTCTTC(1/4)
MaeI 1840 CTAG C^TAG
MaeII 360 ACGT A^CGT
MamI 811 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 76 GAAGA GAAGA(8/7)
MflI 193 GGATCC [GA]^GATC[CT]
NarI 1944 GGCGCC GG^CGCC
NotI 527 GCGGCCGC GC^GGCCGC
NspI 1709 GCATGC [GA]CATG^[CT]
PaeR7I 439 CTCGAG C^TCGAG
RsrII 769 CGGACCG CG^G[AT]CCG
SacI 424 GAGCTC GAGCT^C
SbfI 477 CCTGCAGG CCTGCA^GG
SfoI 1944 GGCGCC GGC^GCC
SphI 1709 GCATGC GCATG^C
Sse8387I 477 CCTGCAGG CCTGCA^GG
TfiI 816 GAATC G^A[AT]TC
XhoI 439 CTCGAG C^TCGAG
XhoII 193 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 202 GGCGTC G[GA]^CG[CT]C
1944 GGCGCC
AlwI 194 GATCC GGATC(4/5)
193 GGATC
Aor51HI 629 AGCGCT AGC^GCT
642 AGCGCT
BalI 701 TGGCCA TGG^CCA
1622 TGGCCA
BcgI 888 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1640 GCAGCCCCATCG
BsaHI 202 GGCGTC G[GA]^CG[CT]C
1944 GGCGCC
BsaI 1135 GAGACC GGTCTC(1/5)
1210 GAGACC
BsgI 1853 CTGCAC GTGCAG(16/14)
1666 GTGCAG
BsmBI 1716 CGTCTC CGTCTC(1/5)
1768 GAGACG
BsrBI 1363 CCGCTC CCGCTC(-3/-3)
517 GAGCGG
BstF5I 960 CATCC GGATG(2/0)
1683 CATCC
DpnII 194 GATC ^GATC
1680 GATC
Eco47III 629 AGCGCT AGC^GCT
642 AGCGCT
FokI 960 CATCC GGATG(9/13)
1683 CATCC
HgaI 203 GCGTC GACGC(5/10)
278 GCGTC
Hin1I 202 GGCGTC G[GA]^CG[CT]C
1944 GGCGCC
MboI 194 GATC ^GATC
1680 GATC
MluNI 701 TGGCCA TGG^CCA
1622 TGGCCA
MscI 701 TGGCCA TGG^CCA
1622 TGGCCA
NaeI 33 GCCGGC GCC^GGC
310 GCCGGC
NdeII 194 GATC ^GATC
1680 GATC
NgoMIV 33 GCCGGC G^CCGGC
310 GCCGGC
PflMI 1243 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1464 CCAGGCTGTGG
Sau3AI 194 GATC ^GATC
1680 GATC
TaqI 440 TCGA T^CGA
558 TCGA
TthHB8I 440 TCGA T^CGA
558 TCGA
Van91I 1243 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1464 CCAGGCTGTGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstAPI BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DsaI EaeI
EagI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KspI
MaeIII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacII Sau96I ScrFI SfcI
SfiI SmaI SmlI StuI StyI
TseI Tsp45I TspRI XcmI XmaCI
XmaI