Restriction map for
pF1KB9610
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AhdI AlwI AscI
AseI AsnI Asp700I AspEI AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstYI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MflI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1053 GGTACC G^GTACC
AccII 893 CGCG CG^CG
AcsI 348 GAATTT [GA]^AATT[CT]
AcyI 520 GGCGTC G[GA]^CG[CT]C
Alw44I 125 GTGCAC G^TGCAC
AocI 1474 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1110 AGCGCT AGC^GCT
ApaLI 125 GTGCAC G^TGCAC
ApoI 348 GAATTT [GA]^AATT[CT]
Asp718I 1053 GGTACC G^GTACC
AspI 1349 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BlpI 1071 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1071 GCTGAGC GC^T[ACGT]AGC
BsaHI 520 GGCGTC G[GA]^CG[CT]C
BsaI 181 GAGACC GGTCTC(1/5)
BseRI 1367 CTCCTC GAGGAG(10/8)
BsmFI 1180 GGGAC GGGAC(10/14)
BsmI 1149 GCATTC GAATGC(1/-1)
BstAPI 1433 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 352 TTCGAA TT^CGAA
BstUI 893 CGCG CG^CG
BstXI 284 CCATGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1474 CCTGAGG CC^T[ACGT]AGG
CelII 1071 GCTGAGC GC^T[ACGT]AGC
DpnII 1223 GATC ^GATC
DraIII 730 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1110 AGCGCT AGC^GCT
Eco81I 1474 CCTGAGG CC^T[ACGT]AGG
HgaI 521 GCGTC GACGC(5/10)
Hin1I 520 GGCGTC G[GA]^CG[CT]C
KpnI 1053 GGTACC GGTAC^C
MboI 1223 GATC ^GATC
MspA1I 1551 CAGCTG C[AC]G^C[GT]G
MvnI 893 CGCG CG^CG
NdeII 1223 GATC ^GATC
NspV 352 TTCGAA TT^CGAA
PflFI 1349 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 482 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1226 CTGCAG CTGCA^G
PvuII 1551 CAGCTG CAG^CTG
Sau3AI 1223 GATC ^GATC
SfuI 352 TTCGAA TT^CGAA
SmlI 760 CTCAAG C^T[CT][GA]AG
TaqI 353 TCGA T^CGA
Tth111I 1349 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 353 TCGA T^CGA
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaI 704 CCCGGG C^[CT]CG[GA]G
1253 CCCGGG
AviII 865 TGCGCA TGC^GCA
974 TGCGCA
BalI 657 TGGCCA TGG^CCA
677 TGGCCA
BsmAI 181 GAGAC GTCTC(1/5)
1122 GAGAC
BsoBI 704 CCCGGG C^[CT]CG[GA]G
1253 CCCGGG
BstEII 816 GGTGACC G^GT[ACGT]ACC
948 GGTGACC
BstPI 816 GGTGACC G^GT[ACGT]ACC
948 GGTGACC
DsaI 852 CCATGG C^C[GA][CT]GG
959 CCATGG
EaeI 657 TGGCCA [CT]^GGCC[GA]
677 TGGCCA
EcoNI 222 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1470 CCTACCTGAGG
EcoO65I 816 GGTGACC G^GT[ACGT]ACC
948 GGTGACC
FspI 865 TGCGCA TGC^GCA
974 TGCGCA
HaeII 662 AGCGCC [GA]GCGC^[CT]
1110 AGCGCT
MluNI 657 TGGCCA TGG^CCA
677 TGGCCA
MscI 657 TGGCCA TGG^CCA
677 TGGCCA
MseI 83 TTAA T^TAA
208 TTAA
MslI 285 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1194 CACCCTTGTG
NcoI 852 CCATGG C^CATGG
959 CCATGG
NspI 548 GCATGT [GA]CATG^[CT]
1438 ACATGC
PleI 333 GACTC GAGTC(4/5)
1404 GACTC
SacI 685 GAGCTC GAGCT^C
1382 GAGCTC
SapI 687 GCTCTTC GCTCTTC(1/4)
1205 GCTCTTC
SfcI 752 CTACAG C^T[GA][CT]AG
1226 CTGCAG
SmaI 704 CCCGGG CCC^GGG
1253 CCCGGG
Tru9I 83 TTAA T^TAA
208 TTAA
Tsp45I 817 GTGAC ^GT[GC]AC
949 GTGAC
XmaCI 704 CCCGGG C^CCGGG
1253 CCCGGG
XmaI 704 CCCGGG C^CCGGG
1253 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
PflMI RsaI Sau96I ScrFI SfaNI
StyI TfiI TseI Tsp509I TspRI
Van91I XcmI