Restriction map for
pF1KB7228
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspHI AspI AvaI
AviII BalI BamHI BbeI BbsI
BcgI BclI BfrI BglI BglII
BpmI BpuAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 772 GGTACC G^GTACC
AciI 451 GCGG CCGC(-3/-1)
AhdI 1661 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 772 GGTACC G^GTACC
AspEI 1661 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1053 GGTCC G^G[AT]CC
AvrII 921 CCTAGG C^CTAGG
BanI 772 GGTACC G^G[CT][GA]CC
BanII 1754 GGGCTC G[GA]GC[CT]^C
BbrPI 700 CACGTG CAC^GTG
BbvCI 988 GCTGAGG CCTCAGC(-5/-2)
BciVI 610 GGATAC GTATCC(6/5)
BcnI 1096 CCCGG CC^[GC]GG
BlnI 921 CCTAGG C^CTAGG
BlpI 411 GCTCAGC GC^T[ACGT]AGC
BmyI 1754 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 411 GCTCAGC GC^T[ACGT]AGC
BsmI 1576 GCATTC GAATGC(1/-1)
Bsp1286I 1754 GGGCTC G[AGT]GC[ACT]^C
BspHI 380 TCATGA T^CATGA
BstAPI 103 GCAGAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 290 CATCC GGATG(2/0)
BstXI 1011 CCAAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 899 GGATCT [GA]^GATC[CT]
Cac8I 1462 GCCAGC GC[ACGT]^[ACGT]GC
CelII 411 GCTCAGC GC^T[ACGT]AGC
EaeI 1511 TGGCCG [CT]^GGCC[GA]
Eam1105I 1661 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1276 GAATTC G^AATTC
EcoRV 1425 GATATC GAT^ATC
FokI 290 CATCC GGATG(9/13)
HapII 1097 CCGG C^CGG
HgaI 369 GACGC GACGC(5/10)
HincII 1669 GTCAAC GT[CT]^[GA]AC
HindII 1669 GTCAAC GT[CT]^[GA]AC
HpaII 1097 CCGG C^CGG
KpnI 772 GGTACC GGTAC^C
MflI 899 GGATCT [GA]^GATC[CT]
MspA1I 869 CAGCTG C[AC]G^C[GT]G
MspI 1097 CCGG C^CGG
NciI 1096 CCCGG CC^[GC]GG
NcoI 434 CCATGG C^CATGG
PflMI 134 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1351 GAGTC GAGTC(4/5)
PmaCI 700 CACGTG CAC^GTG
PmlI 700 CACGTG CAC^GTG
PshAI 1047 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 869 CAGCTG CAG^CTG
RcaI 380 TCATGA T^CATGA
SspI 1497 AATATT AAT^ATT
Van91I 134 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1258 CCACTGATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 899 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AflIII 706 ACACGT A^C[GA][CT]GT
718 ACGTGT
BsaI 1091 GAGACC GGTCTC(1/5)
1774 GAGACC
BseRI 1388 CTCCTC GAGGAG(10/8)
1348 GAGGAG
BsgI 1118 CTGCAC GTGCAG(16/14)
463 GTGCAG
BsmAI 1091 GAGAC GTCTC(1/5)
1774 GAGAC
DsaI 434 CCATGG C^C[GA][CT]GG
534 CCACGG
HindIII 980 AAGCTT A^AGCTT
1015 AAGCTT
MwoI 103 GCAGAATGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
906 GCTTCAGTGGC
NdeI 1622 CATATG CA^TATG
1704 CATATG
NlaIV 772 GGTACC GG[ACGT]^[ACGT]CC
1755 GGCTCC
SfaNI 441 GATGC GCATC(5/9)
232 GCATC
TfiI 926 GATTC G^A[AT]TC
1153 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BfaI BsaAI BsaJI
BsiYI BslI BsrDI BsrI BssKI
BstNI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI