Restriction map for
pF1KB7254
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BcgI BciVI
BfaI BlnI BpmI BsaAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BsrBI BsrDI BsrFI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1525 GACGTC GACGT^C
AccI 934 GTAGAC GT^[AC][GT]AC
AccIII 242 TCCGGA T^CCGGA
AflII 892 CTTAAG C^TTAAG
ApaI 1156 GGGCCC GGGCC^C
Asp700I 1419 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 340 GTGCTC G[AT]GC[AT]^C
AvaI 1323 CTCGGG C^[CT]CG[GA]G
BamHI 1711 GGATCC G^GATCC
BfrI 892 CTTAAG C^TTAAG
BglI 91 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 413 AGATCT A^GATCT
BsaI 1672 GGTCTC GGTCTC(1/5)
BsaWI 242 TCCGGA [AT]^CCGG[AT]
BseAI 242 TCCGGA T^CCGGA
BsiEI 4 CGGCCG CG[GA][CT]^CG
BsiHKAI 340 GTGCTC G[AT]GC[AT]^C
BsoBI 1323 CTCGGG C^[CT]CG[GA]G
BspEI 242 TCCGGA T^CCGGA
BspLU11I 356 ACATGT A^CATGT
BssHII 26 GCGCGC G^CGCGC
BstBI 989 TTCGAA TT^CGAA
BstXI 455 CCACATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 388 GACTTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4 CGGCCG C^GGCCG
EarI 1270 GAAGAG CTCTTC(1/4)
EclXI 4 CGGCCG C^GGCCG
Eco52I 4 CGGCCG C^GGCCG
EcoRI 280 GAATTC G^AATTC
HincII 826 GTCAAC GT[CT]^[GA]AC
HindII 826 GTCAAC GT[CT]^[GA]AC
Ksp632I 1270 GAAGAG CTCTTC(1/4)
MroI 242 TCCGGA T^CCGGA
NcoI 1034 CCATGG C^CATGG
NspV 989 TTCGAA TT^CGAA
PflMI 1448 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 59 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1687 CTGCAG CTGCA^G
PvuII 1813 CAGCTG CAG^CTG
SfcI 1687 CTGCAG C^T[GA][CT]AG
SfuI 989 TTCGAA TT^CGAA
SmlI 892 CTTAAG C^T[CT][GA]AG
Van91I 1448 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1419 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 280 GAATTC [GA]^AATT[CT]
903 AAATTT
AcyI 1525 GACGTC G[GA]^CG[CT]C
1792 GGCGTC
AfaI 1302 GTAC GT^AC
1390 GTAC
AlwNI 864 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1690 CAGACCCTG
ApoI 280 GAATTC [GA]^AATT[CT]
903 AAATTT
BbvCI 639 GCTGAGG CCTCAGC(-5/-2)
1213 GCTGAGG
BclI 926 TGATCA T^GATCA
1052 TGATCA
BcnI 815 CCGGG CC^[GC]GG
1025 CCCGG
BlpI 69 GCTGAGC GC^T[ACGT]AGC
554 GCTCAGC
Bpu1102I 69 GCTGAGC GC^T[ACGT]AGC
554 GCTCAGC
BsaHI 1525 GACGTC G[GA]^CG[CT]C
1792 GGCGTC
BsgI 258 CTGCAC GTGCAG(16/14)
427 CTGCAC
BsmAI 1062 GAGAC GTCTC(1/5)
1673 GTCTC
BsrI 1332 ACTGG ACTGG(1/-1)
1040 CCAGT
BstAPI 251 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
863 GCAGCAACTGC
BstYI 413 AGATCT [GA]^GATC[CT]
1711 GGATCC
CelII 69 GCTGAGC GC^T[ACGT]AGC
554 GCTCAGC
DraII 59 GGGACCT [GA]G^G[ACGT]CC[CT]
1155 AGGGCCC
DraIII 1135 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
1449 CACGCTGTG
DsaI 1034 CCATGG C^C[GA][CT]GG
1139 CCGTGG
EcoO109I 59 GGGACCT [GA]G^G[ACGT]CC[CT]
1155 AGGGCCC
EcoT14I 404 CCTTGG C^C[AT][AT]GG
1034 CCATGG
FauI 469 GCGGG CCCGC(4/6)
1922 GCGGG
FbaI 926 TGATCA T^GATCA
1052 TGATCA
HaeII 99 GGCGCT [GA]GCGC^[CT]
422 AGCGCC
Hin1I 1525 GACGTC G[GA]^CG[CT]C
1792 GGCGTC
MflI 413 AGATCT [GA]^GATC[CT]
1711 GGATCC
MseI 893 TTAA T^TAA
1130 TTAA
NciI 815 CCGGG CC^[GC]GG
1025 CCCGG
NspI 356 ACATGT [GA]CATG^[CT]
1046 GCATGT
PleI 239 GACTC GAGTC(4/5)
718 GAGTC
RsaI 1302 GTAC GT^AC
1390 GTAC
StyI 404 CCTTGG C^C[AT][AT]GG
1034 CCATGG
Tru9I 893 TTAA T^TAA
1130 TTAA
XhoII 413 AGATCT [GA]^GATC[CT]
1711 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AflIII AluI AlwI
AvaII BalI BanI BanII BbsI
BbvI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BspHI
BspMI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV RcaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI