Restriction map for
pF1KE0542
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 472 CGCG CG^CG
AcsI 403 AAATTT [GA]^AATT[CT]
AcyI 698 GGCGTC G[GA]^CG[CT]C
Alw44I 908 GTGCAC G^TGCAC
AlwNI 219 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 908 GTGCAC G^TGCAC
ApoI 403 AAATTT [GA]^AATT[CT]
AvaII 30 GGTCC G^G[AT]CC
BanI 186 GGCACC G^G[CT][GA]CC
BciVI 90 GGATAC GTATCC(6/5)
BfaI 98 CTAG C^TAG
BglII 309 AGATCT A^GATCT
BpmI 157 CTGGAG CTGGAG(16/14)
BsaHI 698 GGCGTC G[GA]^CG[CT]C
BsgI 626 GTGCAG GTGCAG(16/14)
BsmAI 221 GTCTC GTCTC(1/5)
BsmFI 792 GGGAC GGGAC(10/14)
BstEII 654 GGTGACC G^GT[ACGT]ACC
BstPI 654 GGTGACC G^GT[ACGT]ACC
BstUI 472 CGCG CG^CG
BstYI 309 AGATCT [GA]^GATC[CT]
Cac8I 906 GCGTGC GC[ACGT]^[ACGT]GC
Cfr13I 30 GGTCC G^G[ACGT]CC
DdeI 688 CTGAG C^T[ACGT]AG
DrdI 672 GACAGTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 41 TGGCCG [CT]^GGCC[GA]
EcoO65I 654 GGTGACC G^GT[ACGT]ACC
EcoT14I 605 CCTTGG C^C[AT][AT]GG
HapII 44 CCGG C^CGG
HgaI 699 GCGTC GACGC(5/10)
Hin1I 698 GGCGTC G[GA]^CG[CT]C
HincII 287 GTCAAC GT[CT]^[GA]AC
HindII 287 GTCAAC GT[CT]^[GA]AC
HpaII 44 CCGG C^CGG
MaeI 98 CTAG C^TAG
MaeII 777 ACGT A^CGT
MflI 309 AGATCT [GA]^GATC[CT]
MspA1I 875 CAGCTG C[AC]G^C[GT]G
MspI 44 CCGG C^CGG
MvnI 472 CGCG CG^CG
NruI 471 TCGCGA TCG^CGA
PflMI 33 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 875 CAGCTG CAG^CTG
Sau96I 30 GGTCC G^G[ACGT]CC
SfaNI 451 GATGC GCATC(5/9)
SspI 745 AATATT AAT^ATT
StuI 804 AGGCCT AGG^CCT
StyI 605 CCTTGG C^C[AT][AT]GG
TfiI 482 GAATC G^A[AT]TC
Van91I 33 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 190 CCATATCGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 309 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 424 GTAC GT^AC
676 GTAC
AspHI 151 GTGCTC G[AT]GC[AT]^C
908 GTGCAC
BmyI 151 GTGCTC G[AGT]GC[ACT]^C
908 GTGCAC
BsiHKAI 151 GTGCTC G[AT]GC[AT]^C
908 GTGCAC
BsiYI 33 CCACGCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
190 CCATATCGAGG
BslI 33 CCACGCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
190 CCATATCGAGG
Bsp1286I 151 GTGCTC G[AGT]GC[ACT]^C
908 GTGCAC
BsrDI 515 CATTGC GCAATG(2/0)
67 GCAATG
DraI 564 TTTAAA TTT^AAA
579 TTTAAA
DraIII 34 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
145 CACATGGTG
HaeIII 42 GGCC GG^CC
805 GGCC
HindIII 388 AAGCTT A^AGCTT
595 AAGCTT
HphI 654 GGTGA GGTGA(8/7)
762 TCACC
MaeIII 61 GTGAC ^GT[ACGT]AC
655 GTGAC
MslI 648 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
888 CACAGTTATG
NlaIV 46 GGAGCC GG[ACGT]^[ACGT]CC
186 GGCACC
PleI 179 GACTC GAGTC(4/5)
794 GACTC
PstI 667 CTGCAG CTGCA^G
823 CTGCAG
RsaI 424 GTAC GT^AC
676 GTAC
SfcI 667 CTGCAG C^T[GA][CT]AG
823 CTGCAG
TaqI 105 TCGA T^CGA
195 TCGA
Tsp45I 61 GTGAC ^GT[GC]AC
655 GTGAC
TthHB8I 105 TCGA T^CGA
195 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI BbsI BbvI BpuAI BsaJI
BsrI BssKI BstF5I BstNI DpnII
DsaI EcoRII Fnu4HI FokI HinfI
ItaI MboI MboII MnlI MseI
MvaI NdeII NlaIII Sau3AI ScrFI
Tru9I TseI Tsp509I TspRI