Restriction map for
pF1KE0543
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI BspDI
BspEI BsrBI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tsp509I
Tth111I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 776 ACATGT A^C[GA][CT]GT
AspHI 640 GAGCTC G[AT]GC[AT]^C
AvaI 167 CTCGGG C^[CT]CG[GA]G
BalI 92 TGGCCA TGG^CCA
BbeI 485 GGCGCC GGCGC^C
BbsI 159 GAAGAC GAAGAC(2/6)
BbvCI 431 CCTCAGC CCTCAGC(-5/-2)
BfaI 724 CTAG C^TAG
BglI 646 GCCGGTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 159 GAAGAC GAAGAC(2/6)
BseRI 568 CTCCTC GAGGAG(10/8)
BsiEI 707 CGGTCG CG[GA][CT]^CG
BsiHKAI 640 GAGCTC G[AT]GC[AT]^C
BsmAI 575 GAGAC GTCTC(1/5)
BsoBI 167 CTCGGG C^[CT]CG[GA]G
BspHI 839 TCATGA T^CATGA
BspLU11I 776 ACATGT A^CATGT
BsrDI 504 CATTGC GCAATG(2/0)
BsrFI 646 GCCGGT [GA]^CCGG[CT]
BssSI 58 CTCGTG CACGAG(-5/-1)
BstYI 12 GGATCT [GA]^GATC[CT]
Cfr10I 646 GCCGGT [GA]^CCGG[CT]
DsaI 365 CCATGG C^C[GA][CT]GG
EcoNI 765 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 365 CCATGG C^C[AT][AT]GG
KasI 485 GGCGCC G^GCGCC
MaeI 724 CTAG C^TAG
MflI 12 GGATCT [GA]^GATC[CT]
MluNI 92 TGGCCA TGG^CCA
MscI 92 TGGCCA TGG^CCA
MslI 366 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 485 GGCGCC GG^CGCC
NcoI 365 CCATGG C^CATGG
PflMI 345 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 99 GACTC GAGTC(4/5)
PpuMI 451 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 385 CAGCTG CAG^CTG
RcaI 839 TCATGA T^CATGA
SacI 640 GAGCTC GAGCT^C
SfoI 485 GGCGCC GGC^GCC
StuI 762 AGGCCT AGG^CCT
StyI 365 CCATGG C^C[AT][AT]GG
Van91I 345 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 723 TCTAGA T^CTAGA
XhoII 12 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 12 GGATC GGATC(4/5)
788 GGATC
AlwNI 10 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
529 CAGGGCCTG
AvaII 452 GGTCC G^G[AT]CC
826 GGACC
BanI 485 GGCGCC G^G[CT][GA]CC
559 GGTGCC
BcnI 323 CCGGG CC^[GC]GG
858 CCGGG
BpmI 227 CTCCAG CTGGAG(16/14)
447 CTCCAG
BsmFI 825 GGGAC GGGAC(10/14)
362 GTCCC
Bsp1407I 131 TGTACA T^GTACA
779 TGTACA
BspMI 70 ACCTGC ACCTGC(4/8)
691 GCAGGT
BsrGI 131 TGTACA T^GTACA
779 TGTACA
BsrI 463 ACTGG ACTGG(1/-1)
864 ACTGG
BstEII 66 GGTGACC G^GT[ACGT]ACC
354 GGTGACC
BstPI 66 GGTGACC G^GT[ACGT]ACC
354 GGTGACC
BstXI 83 CCATCGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
239 CCATCAAGCTGG
EcoO65I 66 GGTGACC G^GT[ACGT]ACC
354 GGTGACC
HaeII 485 GGCGCC [GA]GCGC^[CT]
847 GGCGCT
MseI 620 TTAA T^TAA
931 TTAA
MspA1I 295 CAGCGG C[AC]G^C[GT]G
385 CAGCTG
NciI 323 CCGGG CC^[GC]GG
858 CCGGG
NspI 188 GCATGT [GA]CATG^[CT]
776 ACATGT
SspBI 131 TGTACA T^GTACA
779 TGTACA
Tru9I 620 TTAA T^TAA
931 TTAA
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcyI AfaI AluI BanII
BbvI BmyI BsaHI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI TseI Tsp45I TspRI TthHB8I