Restriction map for
pF1KE0520
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MamI
MfeI MluI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 545 TCCGGA T^CCGGA
AcyI 296 GGCGCC G[GA]^CG[CT]C
Alw44I 217 GTGCAC G^TGCAC
AlwI 206 GGATC GGATC(4/5)
ApaLI 217 GTGCAC G^TGCAC
BanI 296 GGCGCC G^G[CT][GA]CC
BbeI 296 GGCGCC GGCGC^C
BlpI 777 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 777 GCTCAGC GC^T[ACGT]AGC
BsaAI 934 TACGTG [CT]AC^GT[GA]
BsaHI 296 GGCGCC G[GA]^CG[CT]C
BsaWI 545 TCCGGA [AT]^CCGG[AT]
BseAI 545 TCCGGA T^CCGGA
BseRI 634 GAGGAG GAGGAG(10/8)
BsmAI 341 GTCTC GTCTC(1/5)
BspEI 545 TCCGGA T^CCGGA
BspMI 586 ACCTGC ACCTGC(4/8)
BsrBI 505 CCGCTC CCGCTC(-3/-3)
BsrFI 559 ACCGGC [GA]^CCGG[CT]
BstAPI 314 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 651 GGTGACC G^GT[ACGT]ACC
BstPI 651 GGTGACC G^GT[ACGT]ACC
BstYI 206 GGATCT [GA]^GATC[CT]
CelII 777 GCTCAGC GC^T[ACGT]AGC
Cfr10I 559 ACCGGC [GA]^CCGG[CT]
DpnII 207 GATC ^GATC
EcoNI 624 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 651 GGTGACC G^GT[ACGT]ACC
HgaI 646 GACGC GACGC(5/10)
Hin1I 296 GGCGCC G[GA]^CG[CT]C
KasI 296 GGCGCC G^GCGCC
KspI 594 CCGCGG CCGC^GG
MboI 207 GATC ^GATC
MboII 209 TCTTC GAAGA(8/7)
MflI 206 GGATCT [GA]^GATC[CT]
MroI 545 TCCGGA T^CCGGA
NarI 296 GGCGCC GG^CGCC
NdeII 207 GATC ^GATC
PflMI 852 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 599 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 594 CCGCGG CCGC^GG
Sau3AI 207 GATC ^GATC
SfiI 138 GGCCCGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 296 GGCGCC GGC^GCC
SmlI 601 CTCAAG C^T[CT][GA]AG
SphI 277 GCATGC GCATG^C
Tsp45I 652 GTGAC ^GT[GC]AC
Van91I 852 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 149 CCATGGCAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 206 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccII 595 CGCG CG^CG
648 CGCG
AlwNI 59 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
681 CAGCTTCTG
AocI 112 CCTCAGG CC^T[ACGT]AGG
381 CCTAAGG
ApaI 85 GGGCCC GGGCC^C
137 GGGCCC
AvaI 91 CCCGAG C^[CT]CG[GA]G
140 CCCGAG
BalI 146 TGGCCA TGG^CCA
809 TGGCCA
BanII 85 GGGCCC G[GA]GC[CT]^C
137 GGGCCC
BbvCI 41 CCTCAGC CCTCAGC(-5/-2)
927 CCTCAGC
BpmI 536 CTGGAG CTGGAG(16/14)
715 CTGGAG
BsgI 253 CTGCAC GTGCAG(16/14)
701 GTGCAG
BsmFI 1028 GGGAC GGGAC(10/14)
894 GTCCC
BsoBI 91 CCCGAG C^[CT]CG[GA]G
140 CCCGAG
BsrI 159 ACTGG ACTGG(1/-1)
411 ACTGG
BstUI 595 CGCG CG^CG
648 CGCG
Bsu36I 112 CCTCAGG CC^T[ACGT]AGG
381 CCTAAGG
CfoI 297 GCGC GCG^C
454 GCGC
DraII 85 GGGCCCC [GA]G^G[ACGT]CC[CT]
116 AGGCCCT
Eco81I 112 CCTCAGG CC^T[ACGT]AGG
381 CCTAAGG
EcoO109I 85 GGGCCCC [GA]G^G[ACGT]CC[CT]
116 AGGCCCT
HaeII 296 GGCGCC [GA]GCGC^[CT]
453 GGCGCT
HhaI 297 GCGC GCG^C
454 GCGC
HinP1I 297 GCGC G^CGC
454 GCGC
MaeIII 415 GTTAC ^GT[ACGT]AC
652 GTGAC
MluNI 146 TGGCCA TGG^CCA
809 TGGCCA
MscI 146 TGGCCA TGG^CCA
809 TGGCCA
MvnI 595 CGCG CG^CG
648 CGCG
PstI 525 CTGCAG CTGCA^G
628 CTGCAG
PvuII 62 CAGCTG CAG^CTG
780 CAGCTG
SfaNI 770 GCATC GCATC(5/9)
941 GCATC
SfcI 525 CTGCAG C^T[GA][CT]AG
628 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AspHI AvaII BbvI
BcnI BglI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NlaIII NlaIV NspI PleI
Sau96I ScrFI StyI TseI TspRI