Restriction map for
pF1KB6424
##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 653 GTAGAC GT^[AC][GT]AC
AciI 42 GCGG CCGC(-3/-1)
AcyI 845 GGCGTC G[GA]^CG[CT]C
AflIII 672 ACATGT A^C[GA][CT]GT
AseI 234 ATTAAT AT^TAAT
AsnI 234 ATTAAT AT^TAAT
Asp700I 815 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 287 GGTCC G^G[AT]CC
BciVI 190 GGATAC GTATCC(6/5)
BpmI 551 CTGGAG CTGGAG(16/14)
BsaHI 845 GGCGTC G[GA]^CG[CT]C
BsaJI 293 CCTGGG C^C[ACGT][ACGT]GG
BsmBI 847 CGTCTC CGTCTC(1/5)
BsmI 546 GCATTC GAATGC(1/-1)
BspLU11I 672 ACATGT A^CATGT
BspMI 913 GCAGGT ACCTGC(4/8)
BsrFI 749 GCCGGC [GA]^CCGG[CT]
BstF5I 57 GGATG GGATG(2/0)
Cfr10I 749 GCCGGC [GA]^CCGG[CT]
DraII 286 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 618 CTCTTC CTCTTC(1/4)
EcoO109I 286 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 682 GATATC GAT^ATC
FokI 57 GGATG GGATG(9/13)
HaeIII 157 GGCC GG^CC
HapII 750 CCGG C^CGG
HgaI 846 GCGTC GACGC(5/10)
Hin1I 845 GGCGTC G[GA]^CG[CT]C
HpaII 750 CCGG C^CGG
HphI 297 GGTGA GGTGA(8/7)
Ksp632I 618 CTCTTC CTCTTC(1/4)
MaeII 144 ACGT A^CGT
MboII 619 TCTTC GAAGA(8/7)
MslI 972 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 750 CCGG C^CGG
NaeI 749 GCCGGC GCC^GGC
NgoMIV 749 GCCGGC G^CCGGC
NlaIV 155 GGGGCC GG[ACGT]^[ACGT]CC
PflMI 623 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 286 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 234 ATTAAT AT^TAAT
SfcI 824 CTGTAG C^T[GA][CT]AG
SmlI 221 CTCAAG C^T[CT][GA]AG
SphI 270 GCATGC GCATG^C
Tsp45I 422 GTCAC ^GT[GC]AC
Van91I 623 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 815 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 146 GTAC GT^AC
432 GTAC
BbvI 204 GCTGC GCAGC(8/12)
947 GCTGC
BfaI 163 CTAG C^TAG
208 CTAG
BsgI 767 GTGCAG GTGCAG(16/14)
911 GTGCAG
BsiYI 623 CCAAATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
743 CCACTTGCCGG
BslI 623 CCAAATTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
743 CCACTTGCCGG
BsmAI 676 GTCTC GTCTC(1/5)
848 GTCTC
BspHI 795 TCATGA T^CATGA
930 TCATGA
Cac8I 270 GCATGC GC[ACGT]^[ACGT]GC
749 GCCGGC
Cfr13I 156 GGGCC G^G[ACGT]CC
287 GGTCC
DpnII 772 GATC ^GATC
907 GATC
EcoT22I 181 ATGCAT ATGCA^T
268 ATGCAT
Fnu4HI 204 GCTGC GC^[ACGT]GC
947 GCTGC
ItaI 204 GCTGC GC^[ACGT]GC
947 GCTGC
MaeI 163 CTAG C^TAG
208 CTAG
MboI 772 GATC ^GATC
907 GATC
MwoI 61 GCTCGGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
607 GCTTGTGATGC
NdeII 772 GATC ^GATC
907 GATC
NsiI 181 ATGCAT ATGCA^T
268 ATGCAT
RcaI 795 TCATGA T^CATGA
930 TCATGA
RsaI 146 GTAC GT^AC
432 GTAC
Sau3AI 772 GATC ^GATC
907 GATC
Sau96I 156 GGGCC G^G[ACGT]CC
287 GGTCC
TseI 204 GCTGC G^C[AT]GC
947 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BsmFI BsrI BssKI BstNI
DdeI EcoRII HinfI MaeIII MnlI
MseI MvaI NlaIII NspI PleI
ScrFI SfaNI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I