Restriction map for
pF1KE0346
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI ApaI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BclI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfuI SgrAI SmlI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 565 CGCG CG^CG
AcsI 271 GAATTT [GA]^AATT[CT]
AflIII 282 ACGTGT A^C[GA][CT]GT
AhdI 1156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1078 GTGCAC G^TGCAC
AlwI 1623 GATCC GGATC(4/5)
AocI 1383 CCTGAGG CC^T[ACGT]AGG
ApaLI 1078 GTGCAC G^TGCAC
ApoI 271 GAATTT [GA]^AATT[CT]
AspEI 1156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 388 GGCGCC GGCGC^C
BbvCI 1460 CCTCAGC CCTCAGC(-5/-2)
BciVI 322 GTATCC GTATCC(6/5)
BfaI 26 CTAG C^TAG
BsmBI 88 GAGACG CGTCTC(1/5)
Bsp1407I 1341 TGTACA T^GTACA
BspMI 480 GCAGGT ACCTGC(4/8)
BsrFI 715 GCCGGC [GA]^CCGG[CT]
BsrGI 1341 TGTACA T^GTACA
BstUI 565 CGCG CG^CG
BstXI 188 CCAAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1622 AGATCC [GA]^GATC[CT]
Bsu36I 1383 CCTGAGG CC^T[ACGT]AGG
Cfr10I 715 GCCGGC [GA]^CCGG[CT]
DpnII 1623 GATC ^GATC
DraIII 1810 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1156 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1383 CCTGAGG CC^T[ACGT]AGG
EcoNI 1357 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 707 GCGTC GACGC(5/10)
KasI 388 GGCGCC G^GCGCC
MaeI 26 CTAG C^TAG
MboI 1623 GATC ^GATC
MflI 1622 AGATCC [GA]^GATC[CT]
MvnI 565 CGCG CG^CG
NaeI 715 GCCGGC GCC^GGC
NarI 388 GGCGCC GG^CGCC
NdeI 1897 CATATG CA^TATG
NdeII 1623 GATC ^GATC
NgoMIV 715 GCCGGC G^CCGGC
NheI 25 GCTAGC G^CTAGC
PpuMI 1886 AGGACCC [GA]G^G[AT]CC[CT]
SacI 1305 GAGCTC GAGCT^C
SapI 1053 GCTCTTC GCTCTTC(1/4)
Sau3AI 1623 GATC ^GATC
SfiI 231 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 388 GGCGCC GGC^GCC
SmaI 5 CCCGGG CCC^GGG
SnaBI 319 TACGTA TAC^GTA
SspBI 1341 TGTACA T^GTACA
TfiI 1641 GATTC G^A[AT]TC
Tsp509I 272 AATT ^AATT
XhoII 1622 AGATCC [GA]^GATC[CT]
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 90 GACGTC GACGT^C
754 GACGTC
AfaI 1342 GTAC GT^AC
1413 GTAC
AlwNI 46 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1174 CAGTTCCTG
Aor51HI 667 AGCGCT AGC^GCT
909 AGCGCT
AvaI 5 CCCGGG C^[CT]CG[GA]G
156 CTCGGG
BbrPI 281 CACGTG CAC^GTG
466 CACGTG
BbsI 1609 GAAGAC GAAGAC(2/6)
1248 GTCTTC
BcgI 753 CGACGTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1596 GCAACAGTATCG
BlpI 999 GCTCAGC GC^T[ACGT]AGC
1566 GCTCAGC
Bpu1102I 999 GCTCAGC GC^T[ACGT]AGC
1566 GCTCAGC
BpuAI 1609 GAAGAC GAAGAC(2/6)
1248 GTCTTC
BsaI 460 GAGACC GGTCTC(1/5)
160 GGTCTC
BsgI 1507 CTGCAC GTGCAG(16/14)
1849 GTGCAG
BsoBI 5 CCCGGG C^[CT]CG[GA]G
156 CTCGGG
BstAPI 835 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1851 GCAGGCTATGC
CelII 999 GCTCAGC GC^T[ACGT]AGC
1566 GCTCAGC
DraII 1387 AGGCCCT [GA]G^G[ACGT]CC[CT]
1886 AGGACCC
Eco47III 667 AGCGCT AGC^GCT
909 AGCGCT
EcoO109I 1387 AGGCCCT [GA]G^G[ACGT]CC[CT]
1886 AGGACCC
MslI 35 CACGCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1206 CACATTCGTG
PmaCI 281 CACGTG CAC^GTG
466 CACGTG
PmlI 281 CACGTG CAC^GTG
466 CACGTG
RsaI 1342 GTAC GT^AC
1413 GTAC
SfcI 939 CTACAG C^T[GA][CT]AG
1324 CTACAG
Tsp45I 93 GTCAC ^GT[GC]AC
1432 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AluI AspHI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NcoI NlaIII NlaIV
PleI PvuII Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I
XcmI