################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AciI 11 GCGG CCGC(-3/-1) BamHI 219 GGATCC G^GATCC BanI 54 GGCACC G^G[CT][GA]CC BbvI 33 GCTGC GCAGC(8/12) BmyI 264 GTGCCC G[AGT]GC[ACT]^C BpmI 95 CTGGAG CTGGAG(16/14) BsiYI 21 CCCCTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 21 CCCCTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 264 GTGCCC G[AGT]GC[ACT]^C BstAPI 206 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 321 GGATG GGATG(2/0) BstYI 219 GGATCC [GA]^GATC[CT] DpnII 220 GATC ^GATC EcoT14I 58 CCTTGG C^C[AT][AT]GG FokI 321 GGATG GGATG(9/13) HincII 226 GTTGAC GT[CT]^[GA]AC HindII 226 GTTGAC GT[CT]^[GA]AC HinfI 98 GAGTC G^A[ACGT]TC MboI 220 GATC ^GATC MflI 219 GGATCC [GA]^GATC[CT] MseI 296 TTAA T^TAA MslI 354 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 9 CAGCGG C[AC]G^C[GT]G NdeII 220 GATC ^GATC NspI 359 GCATGT [GA]CATG^[CT] PleI 98 GAGTC GAGTC(4/5) Sau3AI 220 GATC ^GATC StyI 58 CCTTGG C^C[AT][AT]GG Tru9I 296 TTAA T^TAA TseI 33 GCTGC G^C[AT]GC Tsp509I 307 AATT ^AATT XhoII 219 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AluI 93 AGCT AG^CT 393 AGCT AlwI 220 GATCC GGATC(4/5) 219 GGATC BalI 279 TGGCCA TGG^CCA 324 TGGCCA Cac8I 36 GCTTGC GC[ACGT]^[ACGT]GC 332 GCAAGC EaeI 279 TGGCCA [CT]^GGCC[GA] 324 TGGCCA EarI 16 CTCTTC CTCTTC(1/4) 180 GAAGAG Fnu4HI 11 GCGGC GC^[ACGT]GC 33 GCTGC ItaI 11 GCGGC GC^[ACGT]GC 33 GCTGC Ksp632I 16 CTCTTC CTCTTC(1/4) 180 GAAGAG MluNI 279 TGGCCA TGG^CCA 324 TGGCCA MscI 279 TGGCCA TGG^CCA 324 TGGCCA MwoI 55 GCACCTTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 206 GCATCAAATGC NlaIII 354 CATG CATG^ 360 CATG NlaIV 54 GGCACC GG[ACGT]^[ACGT]CC 219 GGATCC SfaNI 291 GATGC GCATC(5/9) 206 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BsaJI BsrI BssKI BstNI DdeI EcoRII HaeIII MaeIII MboII MnlI MvaI ScrFI Tsp45I TspRI