Restriction map for
pF1KB7706
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# NO CUT ENZYMES ( 126 enzymes) #
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Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BcnI BfrI
BglI BlnI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FseI FspI HpaI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 3 GACGTC GACGT^C
AccI 823 GTCTAC GT^[AC][GT]AC
AciI 1033 GCGG CCGC(-3/-1)
Asp700I 853 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1213 GAGCTC G[AT]GC[AT]^C
AvaII 628 GGACC G^G[AT]CC
BalI 11 TGGCCA TGG^CCA
BanI 139 GGTGCC G^G[CT][GA]CC
BciVI 723 GGATAC GTATCC(6/5)
BclI 690 TGATCA T^GATCA
BglII 208 AGATCT A^GATCT
BlpI 1165 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1165 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 1213 GAGCTC G[AT]GC[AT]^C
BsmI 1036 GAATGC GAATGC(1/-1)
Bsp1407I 1062 TGTACA T^GTACA
BspHI 303 TCATGA T^CATGA
BspMI 228 GCAGGT ACCTGC(4/8)
BsrFI 1274 ACCGGC [GA]^CCGG[CT]
BsrGI 1062 TGTACA T^GTACA
BstAPI 100 GCAGTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1165 GCTCAGC GC^T[ACGT]AGC
CfoI 279 GCGC GCG^C
Cfr10I 1274 ACCGGC [GA]^CCGG[CT]
DrdI 629 GACCAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 436 GAATTC G^AATTC
EcoT22I 863 ATGCAT ATGCA^T
FbaI 690 TGATCA T^GATCA
HaeII 278 GGCGCT [GA]GCGC^[CT]
HgaI 221 GACGC GACGC(5/10)
HhaI 279 GCGC GCG^C
HinP1I 279 GCGC G^CGC
HincII 1186 GTCAAC GT[CT]^[GA]AC
HindII 1186 GTCAAC GT[CT]^[GA]AC
HindIII 854 AAGCTT A^AGCTT
MfeI 965 CAATTG C^AATTG
MluNI 11 TGGCCA TGG^CCA
MscI 11 TGGCCA TGG^CCA
MunI 965 CAATTG C^AATTG
NheI 592 GCTAGC G^CTAGC
NsiI 863 ATGCAT ATGCA^T
NspI 497 ACATGC [GA]CATG^[CT]
PflMI 714 CCAGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 303 TCATGA T^CATGA
SacI 1213 GAGCTC GAGCT^C
SapI 796 GAAGAGC GCTCTTC(1/4)
SbfI 225 CCTGCAGG CCTGCA^GG
SmlI 165 CTTGAG C^T[CT][GA]AG
Sse8387I 225 CCTGCAGG CCTGCA^GG
SspBI 1062 TGTACA T^GTACA
TaqI 961 TCGA T^CGA
TthHB8I 961 TCGA T^CGA
Van91I 714 CCAGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 143 CCATGGAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 853 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AcyI 3 GACGTC G[GA]^CG[CT]C
221 GACGCC
AlwI 901 GATCC GGATC(4/5)
343 GGATC
BanII 1213 GAGCTC G[GA]GC[CT]^C
1362 GGGCTC
BmyI 1213 GAGCTC G[AGT]GC[ACT]^C
1362 GGGCTC
BpmI 410 CTCCAG CTGGAG(16/14)
154 CTGGAG
BsaHI 3 GACGTC G[GA]^CG[CT]C
221 GACGCC
BsaJI 143 CCATGG C^C[ACGT][ACGT]GG
1191 CCATGG
BsiYI 714 CCAGCCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1295 CCCCATGTTGG
BslI 714 CCAGCCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1295 CCCCATGTTGG
Bsp1286I 1213 GAGCTC G[AGT]GC[ACT]^C
1362 GGGCTC
BssKI 175 CCAGG ^CC[ACGT]GG
631 CCAGG
BstNI 175 CCAGG CC^[AT]GG
631 CCAGG
Cfr13I 628 GGACC G^G[ACGT]CC
1324 GGGCC
DsaI 143 CCATGG C^C[GA][CT]GG
1191 CCATGG
EaeI 11 TGGCCA [CT]^GGCC[GA]
283 TGGCCG
EarI 1328 CTCTTC CTCTTC(1/4)
796 GAAGAG
EcoRII 175 CCAGG ^CC[AT]GG
631 CCAGG
EcoT14I 143 CCATGG C^C[AT][AT]GG
1191 CCATGG
HapII 286 CCGG C^CGG
1275 CCGG
Hin1I 3 GACGTC G[GA]^CG[CT]C
221 GACGCC
HpaII 286 CCGG C^CGG
1275 CCGG
Ksp632I 1328 CTCTTC CTCTTC(1/4)
796 GAAGAG
MaeII 4 ACGT A^CGT
514 ACGT
MspA1I 653 CAGCTG C[AC]G^C[GT]G
1128 CAGCTG
MspI 286 CCGG C^CGG
1275 CCGG
MvaI 175 CCAGG CC^[AT]GG
631 CCAGG
NcoI 143 CCATGG C^CATGG
1191 CCATGG
PleI 297 GAGTC GAGTC(4/5)
396 GAGTC
PstI 226 CTGCAG CTGCA^G
1131 CTGCAG
PvuII 653 CAGCTG CAG^CTG
1128 CAGCTG
Sau96I 628 GGACC G^G[ACGT]CC
1324 GGGCC
ScrFI 175 CCAGG CC^[ACGT]GG
631 CCAGG
SfaNI 106 GATGC GCATC(5/9)
865 GCATC
SfcI 226 CTGCAG C^T[GA][CT]AG
1131 CTGCAG
SpeI 31 ACTAGT A^CTAGT
874 ACTAGT
StyI 143 CCATGG C^C[AT][AT]GG
1191 CCATGG
Tsp45I 196 GTCAC ^GT[GC]AC
1046 GTGAC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
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AcsI AfaI AluI AlwNI ApoI
BbvI BfaI BseRI BsrI BstF5I
BstYI Cac8I DdeI DpnII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI TfiI
Tru9I TseI Tsp509I TspRI XhoII