Restriction map for
pF1KB7320
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccIII AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BalI BbrPI BbsI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BstEII BstPI Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MfeI MluI MluNI
MroI MscI MspA1I MunI NaeI
NgoMIV NheI NotI NruI NsiI
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 87 CGCG CG^CG
AclI 2584 AACGTT AA^CGTT
Aor51HI 317 AGCGCT AGC^GCT
AspI 2815 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3501 GGATCC G^GATCC
BbeI 73 GGCGCC GGCGC^C
BbvCI 2644 GCTGAGG CCTCAGC(-5/-2)
BciVI 553 GTATCC GTATCC(6/5)
BsaBI 1519 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 108 CGGCCG CG[GA][CT]^CG
BsmI 191 GCATTC GAATGC(1/-1)
Bsp1407I 472 TGTACA T^GTACA
BsrGI 472 TGTACA T^GTACA
BssSI 1221 CACGAG CACGAG(-5/-1)
Bst1107I 1289 GTATAC GTA^TAC
BstNI 1837 CCAGG CC^[AT]GG
BstUI 87 CGCG CG^CG
BstXI 1232 CCAAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1289 GTATAC GTA^TAC
DraII 303 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 645 CCATGG C^C[GA][CT]GG
EaeI 108 CGGCCG [CT]^GGCC[GA]
EagI 108 CGGCCG C^GGCCG
EclXI 108 CGGCCG C^GGCCG
Eco47III 317 AGCGCT AGC^GCT
Eco52I 108 CGGCCG C^GGCCG
EcoO109I 303 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 1837 CCAGG ^CC[AT]GG
EcoRV 1519 GATATC GAT^ATC
FauI 204 CCCGC CCCGC(4/6)
HindIII 2117 AAGCTT A^AGCTT
KasI 73 GGCGCC G^GCGCC
MamI 1519 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 592 CACATAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 1837 CCAGG CC^[AT]GG
MvnI 87 CGCG CG^CG
NarI 73 GGCGCC GG^CGCC
NcoI 645 CCATGG C^CATGG
NdeI 3368 CATATG CA^TATG
PflFI 2815 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 303 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2584 AACGTT AA^CGTT
SfoI 73 GGCGCC GGC^GCC
SspBI 472 TGTACA T^GTACA
Tsp45I 3068 GTCAC ^GT[GC]AC
Tth111I 2815 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1289 GTATAC GT^[AC][GT]AC
3490 GTAGAC
AcyI 73 GGCGCC G[GA]^CG[CT]C
3472 GACGCC
AvaI 1345 CTCGAG C^[CT]CG[GA]G
3481 CTCGAG
AvaII 304 GGACC G^G[AT]CC
660 GGACC
BcnI 32 CCCGG CC^[GC]GG
3242 CCCGG
BsaHI 73 GGCGCC G[GA]^CG[CT]C
3472 GACGCC
BsoBI 1345 CTCGAG C^[CT]CG[GA]G
3481 CTCGAG
BspMI 1293 ACCTGC ACCTGC(4/8)
3409 GCAGGT
BstAPI 136 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2565 GCAGAAATTGC
BstBI 638 TTCGAA TT^CGAA
3752 TTCGAA
EcoT14I 645 CCATGG C^C[AT][AT]GG
929 CCTTGG
HaeII 73 GGCGCC [GA]GCGC^[CT]
317 AGCGCT
HgaI 85 GACGC GACGC(5/10)
3472 GACGC
Hin1I 73 GGCGCC G[GA]^CG[CT]C
3472 GACGCC
HphI 688 GGTGA GGTGA(8/7)
3621 TCACC
NciI 32 CCCGG CC^[GC]GG
3242 CCCGG
NspI 1215 ACATGC [GA]CATG^[CT]
3012 ACATGC
NspV 638 TTCGAA TT^CGAA
3752 TTCGAA
PaeR7I 1345 CTCGAG C^TCGAG
3481 CTCGAG
SacI 233 GAGCTC GAGCT^C
1761 GAGCTC
SfuI 638 TTCGAA TT^CGAA
3752 TTCGAA
StyI 645 CCATGG C^C[AT][AT]GG
929 CCTTGG
XhoI 1345 CTCGAG C^TCGAG
3481 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI BanI BanII BbvI BfaI
BglII BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoNI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MwoI
NdeII NlaIII NlaIV PleI PshBI
PstI RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI