Restriction map for
pF1KB7601
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BsaAI BsaI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MfeI MluI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SapI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 277 GTCGAC GT^[AC][GT]AC
AccIII 405 TCCGGA T^CCGGA
AgeI 794 ACCGGT A^CCGGT
AlwI 224 GGATC GGATC(4/5)
AlwNI 657 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 500 TGGCCA TGG^CCA
BbsI 544 GTCTTC GAAGAC(2/6)
BglII 635 AGATCT A^GATCT
BlpI 424 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 424 GCTGAGC GC^T[ACGT]AGC
BpuAI 544 GTCTTC GAAGAC(2/6)
BsaBI 597 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 405 TCCGGA T^CCGGA
BsmBI 64 GAGACG CGTCTC(1/5)
BspEI 405 TCCGGA T^CCGGA
BstAPI 554 GCAAGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 157 GGTGACC G^GT[ACGT]ACC
BstPI 157 GGTGACC G^GT[ACGT]ACC
BstYI 635 AGATCT [GA]^GATC[CT]
CelII 424 GCTGAGC GC^T[ACGT]AGC
DraIII 363 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 220 CCACGG C^C[GA][CT]GG
EagI 188 CGGCCG C^GGCCG
EclXI 188 CGGCCG C^GGCCG
Eco52I 188 CGGCCG C^GGCCG
EcoNI 650 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 157 GGTGACC G^GT[ACGT]ACC
FauI 824 CCCGC CCCGC(4/6)
HaeII 803 GGCGCT [GA]GCGC^[CT]
HincII 277 GTCGAC GT[CT]^[GA]AC
HindII 277 GTCGAC GT[CT]^[GA]AC
HinfI 853 GAGTC G^A[ACGT]TC
MamI 597 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 545 TCTTC GAAGA(8/7)
MflI 635 AGATCT [GA]^GATC[CT]
MluNI 500 TGGCCA TGG^CCA
MroI 405 TCCGGA T^CCGGA
MscI 500 TGGCCA TGG^CCA
MspA1I 514 CAGCTG C[AC]G^C[GT]G
NaeI 800 GCCGGC GCC^GGC
NgoMIV 800 GCCGGC G^CCGGC
NspI 827 GCATGT [GA]CATG^[CT]
PinAI 794 ACCGGT A^CCGGT
PleI 853 GAGTC GAGTC(4/5)
PpuMI 41 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 514 CAGCTG CAG^CTG
SalI 277 GTCGAC G^TCGAC
SbfI 528 CCTGCAGG CCTGCA^GG
ScaI 557 AGTACT AGT^ACT
SmlI 592 CTCAAG C^T[CT][GA]AG
Sse8387I 528 CCTGCAGG CCTGCA^GG
StuI 647 AGGCCT AGG^CCT
Tsp45I 158 GTGAC ^GT[GC]AC
XhoII 635 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 5 CGCG CG^CG
842 CGCG
AcyI 256 GACGCC G[GA]^CG[CT]C
781 GACGCC
AluI 234 AGCT AG^CT
515 AGCT
BglI 289 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
493 GCCGGGCTGGC
BsaHI 256 GACGCC G[GA]^CG[CT]C
781 GACGCC
BsaWI 405 TCCGGA [AT]^CCGG[AT]
794 ACCGGT
BsiEI 188 CGGCCG CG[GA][CT]^CG
741 CGACCG
BsmAI 64 GAGAC GTCTC(1/5)
131 GAGAC
BstF5I 603 CATCC GGATG(2/0)
431 GGATG
BstUI 5 CGCG CG^CG
842 CGCG
DdeI 425 CTGAG C^T[ACGT]AG
777 CTCAG
DpnII 225 GATC ^GATC
636 GATC
EcoT14I 260 CCTTGG C^C[AT][AT]GG
380 CCTTGG
FokI 603 CATCC GGATG(9/13)
431 GGATG
Hin1I 256 GACGCC G[GA]^CG[CT]C
781 GACGCC
MaeIII 158 GTGAC ^GT[ACGT]AC
666 GTTAC
MboI 225 GATC ^GATC
636 GATC
MvnI 5 CGCG CG^CG
842 CGCG
NdeII 225 GATC ^GATC
636 GATC
Sau3AI 225 GATC ^GATC
636 GATC
SfiI 288 GGCCAGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
492 GGCCGGGCTGGCC
SmaI 444 CCCGGG CCC^GGG
867 CCCGGG
StyI 260 CCTTGG C^C[AT][AT]GG
380 CCTTGG
TaqI 227 TCGA T^CGA
278 TCGA
TthHB8I 227 TCGA T^CGA
278 TCGA
XmaCI 444 CCCGGG C^CCGGG
867 CCCGGG
XmaI 444 CCCGGG C^CCGGG
867 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI ApaI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BssKI BstNI Cac8I
CfoI Cfr10I Cfr13I DraII EaeI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MnlI MspI MvaI MwoI
NciI NlaIII NlaIV PflMI PstI
RsaI Sau96I ScrFI SfaNI SfcI
TseI TspRI Van91I