Restriction map for
pF1KB6320
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KspI MaeI MaeII MaeIII
MamI MfeI MluI MroI MspA1I
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI StyI SwaI TaqI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 717 GGTACC G^GTACC
AccII 781 CGCG CG^CG
AcyI 447 GGCGCC G[GA]^CG[CT]C
AlwNI 115 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 717 GGTACC G^GTACC
AspHI 661 GAGCTC G[AT]GC[AT]^C
BanII 661 GAGCTC G[GA]GC[CT]^C
BbeI 447 GGCGCC GGCGC^C
BciVI 248 GTATCC GTATCC(6/5)
BglII 827 AGATCT A^GATCT
BmyI 661 GAGCTC G[AGT]GC[ACT]^C
BsaHI 447 GGCGCC G[GA]^CG[CT]C
BseRI 649 GAGGAG GAGGAG(10/8)
BsiHKAI 661 GAGCTC G[AT]GC[AT]^C
Bsp1286I 661 GAGCTC G[AGT]GC[ACT]^C
BspMI 641 ACCTGC ACCTGC(4/8)
BsrBI 307 GAGCGG CCGCTC(-3/-3)
BsrDI 279 CATTGC GCAATG(2/0)
BstF5I 69 GGATG GGATG(2/0)
BstUI 781 CGCG CG^CG
BstXI 389 CCACCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 414 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 395 CCGTGG C^C[GA][CT]GG
EarI 327 GAAGAG CTCTTC(1/4)
EcoNI 642 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 414 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 69 GGATG GGATG(9/13)
Hin1I 447 GGCGCC G[GA]^CG[CT]C
HphI 635 TCACC GGTGA(8/7)
KasI 447 GGCGCC G^GCGCC
KpnI 717 GGTACC GGTAC^C
Ksp632I 327 GAAGAG CTCTTC(1/4)
MseI 800 TTAA T^TAA
MvnI 781 CGCG CG^CG
NarI 447 GGCGCC GG^CGCC
NruI 780 TCGCGA TCG^CGA
PflMI 114 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 790 CTGCAG CTGCA^G
SacI 661 GAGCTC GAGCT^C
SbfI 789 CCTGCAGG CCTGCA^GG
SfcI 790 CTGCAG C^T[GA][CT]AG
SfoI 447 GGCGCC GGC^GCC
SphI 428 GCATGC GCATG^C
Sse8387I 789 CCTGCAGG CCTGCA^GG
Tru9I 800 TTAA T^TAA
Van91I 114 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 389 CCACCCCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AvaII 312 GGACC G^G[AT]CC
708 GGTCC
BalI 111 TGGCCA TGG^CCA
531 TGGCCA
BsrI 105 CCAGT ACTGG(1/-1)
252 CCAGT
BstAPI 462 GCACCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
564 GCAGCTTATGC
EcoRV 124 GATATC GAT^ATC
274 GATATC
HaeII 190 AGCGCC [GA]GCGC^[CT]
447 GGCGCC
MluNI 111 TGGCCA TGG^CCA
531 TGGCCA
MscI 111 TGGCCA TGG^CCA
531 TGGCCA
MslI 297 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
390 CACCCCCGTG
PleI 268 GACTC GAGTC(4/5)
441 GACTC
SfaNI 582 GATGC GCATC(5/9)
784 GATGC
SmaI 423 CCCGGG CCC^GGG
721 CCCGGG
StuI 161 AGGCCT AGG^CCT
519 AGGCCT
TfiI 609 GAATC G^A[AT]TC
618 GAATC
XmaCI 423 CCCGGG C^CCGGG
721 CCCGGG
XmaI 423 CCCGGG C^CCGGG
721 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BanI BbvI BcnI BsaI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
BssKI BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
TseI XhoII