################################## # NO CUT ENZYMES ( 214 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MroI MseI MslI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccII 86 CGCG CG^CG AfaI 6 GTAC GT^AC AlwI 227 GGATC GGATC(4/5) Asp700I 184 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 12 TGGCCA TGG^CCA BbvI 45 GCAGC GCAGC(8/12) BsiYI 9 CCGTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 9 CCGTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 268 GTCTC GTCTC(1/5) BsrI 219 ACTGG ACTGG(1/-1) BssKI 15 CCAGG ^CC[ACGT]GG BstNI 15 CCAGG CC^[AT]GG BstUI 86 CGCG CG^CG DsaI 9 CCGTGG C^C[GA][CT]GG EaeI 12 TGGCCA [CT]^GGCC[GA] EarI 184 GAAGAG CTCTTC(1/4) EcoRII 15 CCAGG ^CC[AT]GG Fnu4HI 45 GCAGC GC^[ACGT]GC HaeIII 13 GGCC GG^CC ItaI 45 GCAGC GC^[ACGT]GC Ksp632I 184 GAAGAG CTCTTC(1/4) MluNI 12 TGGCCA TGG^CCA MnlI 57 CCTC CCTC(7/6) MscI 12 TGGCCA TGG^CCA MvaI 15 CCAGG CC^[AT]GG MvnI 86 CGCG CG^CG MwoI 102 GCTCTATGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 231 CATG CATG^ RsaI 6 GTAC GT^AC ScrFI 15 CCAGG CC^[ACGT]GG SfaNI 178 GATGC GCATC(5/9) SspI 247 AATATT AAT^ATT TseI 45 GCAGC G^C[AT]GC XmnI 184 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AciI 87 GCGG CCGC(-3/-1) 123 GCGG BsaJI 9 CCGTGG C^C[ACGT][ACGT]GG 15 CCAGGG BsgI 28 GTGCAG GTGCAG(16/14) 43 GTGCAG DpnII 83 GATC ^GATC 228 GATC HinfI 90 GATTC G^A[ACGT]TC 189 GATTC MboI 83 GATC ^GATC 228 GATC MboII 184 GAAGA GAAGA(8/7) 62 TCTTC NdeII 83 GATC ^GATC 228 GATC Sau3AI 83 GATC ^GATC 228 GATC TfiI 90 GATTC G^A[AT]TC 189 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 0 enzymes) # #########################################