Restriction map for pF1KB6729
##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MroI        MseI        MslI        MspA1I      MspI        
MunI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TaqI        Tru9I       Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccII          86  CGCG                 CG^CG
AfaI            6  GTAC                 GT^AC
AlwI          227  GGATC                GGATC(4/5)
Asp700I       184  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI           12  TGGCCA               TGG^CCA
BbvI           45  GCAGC                GCAGC(8/12)
BsiYI           9  CCGTGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI            9  CCGTGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         268  GTCTC                GTCTC(1/5)
BsrI          219  ACTGG                ACTGG(1/-1)
BssKI          15  CCAGG                ^CC[ACGT]GG
BstNI          15  CCAGG                CC^[AT]GG
BstUI          86  CGCG                 CG^CG
DsaI            9  CCGTGG               C^C[GA][CT]GG
EaeI           12  TGGCCA               [CT]^GGCC[GA]
EarI          184  GAAGAG               CTCTTC(1/4)
EcoRII         15  CCAGG                ^CC[AT]GG
Fnu4HI         45  GCAGC                GC^[ACGT]GC
HaeIII         13  GGCC                 GG^CC
ItaI           45  GCAGC                GC^[ACGT]GC
Ksp632I       184  GAAGAG               CTCTTC(1/4)
MluNI          12  TGGCCA               TGG^CCA
MnlI           57  CCTC                 CCTC(7/6)
MscI           12  TGGCCA               TGG^CCA
MvaI           15  CCAGG                CC^[AT]GG
MvnI           86  CGCG                 CG^CG
MwoI          102  GCTCTATGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        231  CATG                 CATG^
RsaI            6  GTAC                 GT^AC
ScrFI          15  CCAGG                CC^[ACGT]GG
SfaNI         178  GATGC                GCATC(5/9)
SspI          247  AATATT               AAT^ATT
TseI           45  GCAGC                G^C[AT]GC
XmnI          184  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI           87  GCGG                 CCGC(-3/-1)
              123  GCGG                
BsaJI           9  CCGTGG               C^C[ACGT][ACGT]GG
               15  CCAGGG              
BsgI           28  GTGCAG               GTGCAG(16/14)
               43  GTGCAG              
DpnII          83  GATC                 ^GATC
              228  GATC                
HinfI          90  GATTC                G^A[ACGT]TC
              189  GATTC               
MboI           83  GATC                 ^GATC
              228  GATC                
MboII         184  GAAGA                GAAGA(8/7)
               62  TCTTC               
NdeII          83  GATC                 ^GATC
              228  GATC                
Sau3AI         83  GATC                 ^GATC
              228  GATC                
TfiI           90  GATTC                G^A[AT]TC
              189  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################


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