##################################
# NO CUT ENZYMES (  230 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FbaI        Fnu4HI      FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        HpaII       ItaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MaeIII      MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        TfiI        TseI        Tsp45I      TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AciI           93  GCGG                 CCGC(-3/-1)
AcsI           31  AAATTT               [GA]^AATT[CT]
AflIII         75  ACATGT               A^C[GA][CT]GT
AluI            9  AGCT                 AG^CT
AlwI          103  GATCC                GGATC(4/5)
ApoI           31  AAATTT               [GA]^AATT[CT]
AvaI           19  CCCGAG               C^[CT]CG[GA]G
BfaI          149  CTAG                 C^TAG
BsoBI          19  CCCGAG               C^[CT]CG[GA]G
BspLU11I       75  ACATGT               A^CATGT
DpnII         103  GATC                 ^GATC
FauI           93  GCGGG                CCCGC(4/6)
HphI           12  TCACC                GGTGA(8/7)
MaeI          149  CTAG                 C^TAG
MboI          103  GATC                 ^GATC
MseI           61  TTAA                 T^TAA
NdeII         103  GATC                 ^GATC
NspI           75  ACATGT               [GA]CATG^[CT]
Sau3AI        103  GATC                 ^GATC
SpeI          148  ACTAGT               A^CTAGT
Tru9I          61  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BsaJI          81  CCAAGG               C^C[ACGT][ACGT]GG
              215  CCTTGG              
EcoT14I        81  CCAAGG               C^C[AT][AT]GG
              215  CCTTGG              
MnlI           16  CCTC                 CCTC(7/6)
              188  GAGG                
NlaIII         76  CATG                 CATG^
              204  CATG                
SspI           87  AATATT               AAT^ATT
              166  AATATT              
StyI           81  CCAAGG               C^C[AT][AT]GG
              215  CCTTGG              
Tsp509I        32  AATT                 ^AATT
               59  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################