Restriction map for
pF1KB7823
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AviII
AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BseAI BsiEI BsiHKAI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
FauI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeIII MamI MluI
MroI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PleI PmeI PpuMI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1448 GTAGAC GT^[AC][GT]AC
AflIII 1370 ACATGT A^C[GA][CT]GT
AlwNI 1115 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 640 ATTAAT AT^TAAT
AsnI 640 ATTAAT AT^TAAT
AspI 325 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 656 CCCGGG C^[CT]CG[GA]G
BalI 257 TGGCCA TGG^CCA
BbrPI 1046 CACGTG CAC^GTG
BclI 1299 TGATCA T^GATCA
BglI 853 GCCAAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 914 GTGCCC G[AGT]GC[ACT]^C
BsaAI 1046 CACGTG [CT]AC^GT[GA]
BsaI 102 GGTCTC GGTCTC(1/5)
BsgI 23 CTGCAC GTGCAG(16/14)
BsiWI 128 CGTACG C^GTACG
BsoBI 656 CCCGGG C^[CT]CG[GA]G
Bsp1286I 914 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 1370 ACATGT A^CATGT
BsrDI 876 CATTGC GCAATG(2/0)
EaeI 257 TGGCCA [CT]^GGCC[GA]
EcoRV 1159 GATATC GAT^ATC
EcoT22I 688 ATGCAT ATGCA^T
FbaI 1299 TGATCA T^GATCA
HaeII 3 GGCGCT [GA]GCGC^[CT]
MfeI 299 CAATTG C^AATTG
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MspA1I 500 CAGCTG C[AC]G^C[GT]G
MunI 299 CAATTG C^AATTG
NsiI 688 ATGCAT ATGCA^T
NspI 1370 ACATGT [GA]CATG^[CT]
PflFI 325 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1046 CACGTG CAC^GTG
PmlI 1046 CACGTG CAC^GTG
PshAI 793 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 640 ATTAAT AT^TAAT
PvuII 500 CAGCTG CAG^CTG
SexAI 353 ACCTGGT A^CC[AT]GGT
SfaNI 295 GATGC GCATC(5/9)
SfcI 823 CTACAG C^T[GA][CT]AG
SmaI 656 CCCGGG CCC^GGG
SmlI 52 CTTGAG C^T[CT][GA]AG
Tth111I 325 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1349 CCAGTAAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 656 CCCGGG C^CCGGG
XmaI 656 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 479 CCGC CCGC(-3/-1)
1563 CCGC
AfaI 129 GTAC GT^AC
832 GTAC
BcnI 656 CCCGG CC^[GC]GG
657 CCGGG
BfaI 698 CTAG C^TAG
1387 CTAG
BpmI 569 CTGGAG CTGGAG(16/14)
1308 CTGGAG
BsaWI 137 ACCGGA [AT]^CCGG[AT]
1097 ACCGGA
BsmAI 1086 GAGAC GTCTC(1/5)
103 GTCTC
BsmFI 228 GGGAC GGGAC(10/14)
660 GGGAC
BssSI 1576 CACGAG CACGAG(-5/-1)
893 CTCGTG
BstXI 260 CCATTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1244 CCAAGGGTATGG
Cac8I 255 GCTGGC GC[ACGT]^[ACGT]GC
912 GCGTGC
HphI 1378 GGTGA GGTGA(8/7)
8 TCACC
MaeI 698 CTAG C^TAG
1387 CTAG
MaeII 798 ACGT A^CGT
1047 ACGT
NciI 656 CCCGG CC^[GC]GG
657 CCGGG
RsaI 129 GTAC GT^AC
832 GTAC
SapI 1001 GAAGAGC GCTCTTC(1/4)
1230 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BciVI BsaJI BseRI BsiYI
BslI BsmI BspMI BsrI BssKI
BstF5I BstNI BstYI CfoI Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII