##################################
# NO CUT ENZYMES (  223 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        ItaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIII      NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AluI           66  AGCT                 AG^CT
AspI          130  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BpmI           62  CTGGAG               CTGGAG(16/14)
BsiYI         133  CCTGTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          133  CCTGTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         142  GGGAC                GGGAC(10/14)
BsmI          169  GAATGC               GAATGC(1/-1)
BsrI           61  ACTGG                ACTGG(1/-1)
BssKI          75  CCTGG                ^CC[ACGT]GG
BstF5I        112  GGATG                GGATG(2/0)
BstNI          75  CCTGG                CC^[AT]GG
DdeI           25  CTCAG                C^T[ACGT]AG
EarI           80  GAAGAG               CTCTTC(1/4)
EcoRII         75  CCTGG                ^CC[AT]GG
EcoT14I        32  CCTTGG               C^C[AT][AT]GG
FokI          112  GGATG                GGATG(9/13)
Ksp632I        80  GAAGAG               CTCTTC(1/4)
MseI          107  TTAA                 T^TAA
MvaI           75  CCTGG                CC^[AT]GG
PflFI         130  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
ScrFI          75  CCTGG                CC^[ACGT]GG
StyI           32  CCTTGG               C^C[AT][AT]GG
Tru9I         107  TTAA                 T^TAA
Tth111I       130  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

Cfr13I         36  GGCCC                G^G[ACGT]CC
              229  GGGCC               
HinfI          45  GAGTC                G^A[ACGT]TC
               70  GACTC               
MboII          80  GAAGA                GAAGA(8/7)
               21  TCTTC               
MnlI          213  CCTC                 CCTC(7/6)
               85  GAGG                
PleI           70  GACTC                GAGTC(4/5)
               45  GAGTC               
Sau96I         36  GGCCC                G^G[ACGT]CC
              229  GGGCC               
SfaNI         113  GATGC                GCATC(5/9)
               14  GCATC               
TspRI          27  CAGTG                CA[GC]TG[ACGT][ACGT]^
               60  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

BsaJI       HaeIII      MaeIII