Restriction map for
pF1KB7778
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1185 GACGTC GACGT^C
AccI 582 GTATAC GT^[AC][GT]AC
AhdI 244 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1084 GTGCAC G^TGCAC
AlwI 1401 GATCC GGATC(4/5)
ApaLI 1084 GTGCAC G^TGCAC
Asp700I 1293 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 244 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1084 GTGCAC G[AT]GC[AT]^C
BclI 614 TGATCA T^GATCA
BglI 22 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 732 AGATCT A^GATCT
BsaBI 312 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 68 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1084 GTGCAC G[AT]GC[AT]^C
BsmBI 1106 CGTCTC CGTCTC(1/5)
BsmI 1293 GAATGC GAATGC(1/-1)
Bst1107I 582 GTATAC GTA^TAC
BstZ17I 582 GTATAC GTA^TAC
DdeI 304 CTGAG C^T[ACGT]AG
Eam1105I 244 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 776 CCAAGG C^C[AT][AT]GG
FbaI 614 TGATCA T^GATCA
MaeII 1186 ACGT A^CGT
MamI 312 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 792 GCCGGC GCC^GGC
NgoMIV 792 GCCGGC G^CCGGC
NspI 1236 GCATGC [GA]CATG^[CT]
PflMI 1425 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 604 CTGCAG CTGCA^G
PvuII 1012 CAGCTG CAG^CTG
SexAI 743 ACCAGGT A^CC[AT]GGT
SphI 1236 GCATGC GCATG^C
StuI 512 AGGCCT AGG^CCT
StyI 776 CCAAGG C^C[AT][AT]GG
TaqI 452 TCGA T^CGA
TfiI 642 GATTC G^A[AT]TC
TthHB8I 452 TCGA T^CGA
Van91I 1425 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1293 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AlwNI 544 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
914 CAGTGTCTG
BalI 560 TGGCCA TGG^CCA
1212 TGGCCA
BbeI 204 GGCGCC GGCGC^C
274 GGCGCC
BbsI 414 GAAGAC GAAGAC(2/6)
492 GAAGAC
BpmI 1149 CTCCAG CTGGAG(16/14)
166 CTGGAG
BpuAI 414 GAAGAC GAAGAC(2/6)
492 GAAGAC
BsaI 625 GAGACC GGTCTC(1/5)
772 GAGACC
BsgI 1069 GTGCAG GTGCAG(16/14)
1396 GTGCAG
BsiEI 85 CGGCCG CG[GA][CT]^CG
159 CGGCCG
BspHI 503 TCATGA T^CATGA
1181 TCATGA
BstXI 899 CCAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1199 CCACAACTGTGG
BstYI 732 AGATCT [GA]^GATC[CT]
1400 AGATCC
DsaI 23 CCGCGG C^C[GA][CT]GG
212 CCGCGG
EagI 85 CGGCCG C^GGCCG
159 CGGCCG
EarI 724 GAAGAG CTCTTC(1/4)
969 GAAGAG
EclXI 85 CGGCCG C^GGCCG
159 CGGCCG
Eco52I 85 CGGCCG C^GGCCG
159 CGGCCG
KasI 204 GGCGCC G^GCGCC
274 GGCGCC
Ksp632I 724 GAAGAG CTCTTC(1/4)
969 GAAGAG
KspI 23 CCGCGG CCGC^GG
212 CCGCGG
MflI 732 AGATCT [GA]^GATC[CT]
1400 AGATCC
MluNI 560 TGGCCA TGG^CCA
1212 TGGCCA
MscI 560 TGGCCA TGG^CCA
1212 TGGCCA
NarI 204 GGCGCC GG^CGCC
274 GGCGCC
PpuMI 128 GGGTCCC [GA]G^G[AT]CC[CT]
1061 AGGTCCC
RcaI 503 TCATGA T^CATGA
1181 TCATGA
SacII 23 CCGCGG CCGC^GG
212 CCGCGG
SfaNI 500 GCATC GCATC(5/9)
1381 GCATC
SfoI 204 GGCGCC GGC^GCC
274 GGCGCC
SmaI 133 CCCGGG CCC^GGG
419 CCCGGG
XcmI 1256 CCACCTCGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1344 CCAGGAGCCAGGTGG
XhoII 732 AGATCT [GA]^GATC[CT]
1400 AGATCC
XmaCI 133 CCCGGG C^CCGGG
419 CCCGGG
XmaI 133 CCCGGG C^CCGGG
419 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfcI
TseI Tsp45I TspRI