Restriction map for
pF1KE0412
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HindIII HpaI HphI KasI Ksp632I
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 659 GGTACC G^GTACC
AcyI 58 GACGCC G[GA]^CG[CT]C
AflIII 343 ACATGT A^C[GA][CT]GT
AlwI 272 GGATC GGATC(4/5)
Asp718I 659 GGTACC G^GTACC
BanI 659 GGTACC G^G[CT][GA]CC
BbsI 667 GTCTTC GAAGAC(2/6)
BfaI 244 CTAG C^TAG
BlpI 513 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 513 GCTCAGC GC^T[ACGT]AGC
BpuAI 667 GTCTTC GAAGAC(2/6)
BsaHI 58 GACGCC G[GA]^CG[CT]C
BsaI 466 GAGACC GGTCTC(1/5)
BsiEI 50 CGGCCG CG[GA][CT]^CG
BsiYI 85 CCGAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 85 CCGAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 251 GGGAC GGGAC(10/14)
BspLU11I 343 ACATGT A^CATGT
BspMI 136 ACCTGC ACCTGC(4/8)
BsrBI 511 CCGCTC CCGCTC(-3/-3)
CelII 513 GCTCAGC GC^T[ACGT]AGC
EagI 50 CGGCCG C^GGCCG
EclXI 50 CGGCCG C^GGCCG
Eco52I 50 CGGCCG C^GGCCG
EcoT14I 107 CCAAGG C^C[AT][AT]GG
Hin1I 58 GACGCC G[GA]^CG[CT]C
HincII 331 GTTGAC GT[CT]^[GA]AC
HindII 331 GTTGAC GT[CT]^[GA]AC
KpnI 659 GGTACC GGTAC^C
KspI 53 CCGCGG CCGC^GG
MaeI 244 CTAG C^TAG
MseI 602 TTAA T^TAA
NaeI 48 GCCGGC GCC^GGC
NgoMIV 48 GCCGGC G^CCGGC
NspI 343 ACATGT [GA]CATG^[CT]
PleI 478 GACTC GAGTC(4/5)
PpuMI 520 AGGACCT [GA]G^G[AT]CC[CT]
PstI 175 CTGCAG CTGCA^G
PvuII 178 CAGCTG CAG^CTG
SacII 53 CCGCGG CCGC^GG
SexAI 662 ACCAGGT A^CC[AT]GGT
SfcI 175 CTGCAG C^T[GA][CT]AG
StyI 107 CCAAGG C^C[AT][AT]GG
TaqI 476 TCGA T^CGA
TfiI 597 GATTC G^A[AT]TC
Tru9I 602 TTAA T^TAA
TthHB8I 476 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 22 CGCG CG^CG
54 CGCG
AfaI 551 GTAC GT^AC
660 GTAC
AvaII 134 GGACC G^G[AT]CC
521 GGACC
BcnI 131 CCCGG CC^[GC]GG
534 CCGGG
BsmAI 466 GAGAC GTCTC(1/5)
319 GTCTC
BsrFI 48 GCCGGC [GA]^CCGG[CT]
149 GCCGGT
BsrI 562 ACTGG ACTGG(1/-1)
486 CCAGT
BstF5I 588 CATCC GGATG(2/0)
537 GGATG
BstUI 22 CGCG CG^CG
54 CGCG
Cfr10I 48 GCCGGC [GA]^CCGG[CT]
149 GCCGGT
DpnII 273 GATC ^GATC
726 GATC
FokI 588 CATCC GGATG(9/13)
537 GGATG
HgaI 58 GACGC GACGC(5/10)
557 GCGTC
HinfI 478 GACTC G^A[ACGT]TC
597 GATTC
MboI 273 GATC ^GATC
726 GATC
MboII 87 GAAGA GAAGA(8/7)
668 TCTTC
MslI 192 CATCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
588 CATCCAGATG
MvnI 22 CGCG CG^CG
54 CGCG
NciI 131 CCCGG CC^[GC]GG
534 CCGGG
NdeII 273 GATC ^GATC
726 GATC
RsaI 551 GTAC GT^AC
660 GTAC
Sau3AI 273 GATC ^GATC
726 GATC
SfaNI 191 GCATC GCATC(5/9)
227 GCATC
Tsp509I 353 AATT ^AATT
604 AATT
TspRI 487 CAGTG CA[GC]TG[ACGT][ACGT]^
561 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI ApaI BanII BbvI
BmyI BsaJI BseRI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HpaII ItaI
MaeIII MnlI MspA1I MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI Tsp45I