Restriction map for
pF1KB7244
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmFI
BsmI BspDI BspEI BspLU11I BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HinfI HpaI Ksp632I
MaeI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 256 GACGTC GACGT^C
Acc65I 550 GGTACC G^GTACC
Alw44I 424 GTGCAC G^TGCAC
ApaI 110 GGGCCC GGGCC^C
ApaLI 424 GTGCAC G^TGCAC
Asp718I 550 GGTACC G^GTACC
AspI 355 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1016 GGATCC G^GATCC
BlpI 432 GCTGAGC GC^T[ACGT]AGC
BpmI 62 CTGGAG CTGGAG(16/14)
Bpu1102I 432 GCTGAGC GC^T[ACGT]AGC
BsaAI 187 TACGTG [CT]AC^GT[GA]
BseRI 42 CTCCTC GAGGAG(10/8)
BsgI 538 GTGCAG GTGCAG(16/14)
BsmAI 498 GTCTC GTCTC(1/5)
Bsp1407I 263 TGTACA T^GTACA
BsrGI 263 TGTACA T^GTACA
BstYI 1016 GGATCC [GA]^GATC[CT]
CelII 432 GCTGAGC GC^T[ACGT]AGC
DdeI 433 CTGAG C^T[ACGT]AG
DpnII 1017 GATC ^GATC
EcoNI 960 CCTCCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 980 CCAAGG C^C[AT][AT]GG
KpnI 550 GGTACC GGTAC^C
KspI 482 CCGCGG CCGC^GG
MboI 1017 GATC ^GATC
MflI 1016 GGATCC [GA]^GATC[CT]
NdeII 1017 GATC ^GATC
PflFI 355 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 491 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 435 GAGCTC GAGCT^C
SacII 482 CCGCGG CCGC^GG
Sau3AI 1017 GATC ^GATC
SspBI 263 TGTACA T^GTACA
StyI 980 CCAAGG C^C[AT][AT]GG
Tth111I 355 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1016 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 1017 GATCC GGATC(4/5)
1016 GGATC
AspHI 424 GTGCAC G[AT]GC[AT]^C
435 GAGCTC
AvaI 48 CCCGGG C^[CT]CG[GA]G
113 CCCGGG
AvaII 405 GGACC G^G[AT]CC
492 GGTCC
BalI 287 TGGCCA TGG^CCA
464 TGGCCA
BglI 466 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
576 GCCCGTGGGGC
BsiEI 155 CGGCCG CG[GA][CT]^CG
614 CGGCCG
BsiHKAI 424 GTGCAC G[AT]GC[AT]^C
435 GAGCTC
BsoBI 48 CCCGGG C^[CT]CG[GA]G
113 CCCGGG
BspHI 344 TCATGA T^CATGA
395 TCATGA
BsrBI 517 CCGCTC CCGCTC(-3/-3)
727 GAGCGG
BsrFI 93 GCCGGC [GA]^CCGG[CT]
847 GCCGGC
BsrI 366 CCAGT ACTGG(1/-1)
374 CCAGT
BstF5I 870 CATCC GGATG(2/0)
775 GGATG
Cfr10I 93 GCCGGC [GA]^CCGG[CT]
847 GCCGGC
DraII 109 GGGGCCC [GA]G^G[ACGT]CC[CT]
491 GGGTCCT
EagI 155 CGGCCG C^GGCCG
614 CGGCCG
EclXI 155 CGGCCG C^GGCCG
614 CGGCCG
Eco52I 155 CGGCCG C^GGCCG
614 CGGCCG
EcoO109I 109 GGGGCCC [GA]G^G[ACGT]CC[CT]
491 GGGTCCT
FokI 870 CATCC GGATG(9/13)
775 GGATG
MluNI 287 TGGCCA TGG^CCA
464 TGGCCA
MscI 287 TGGCCA TGG^CCA
464 TGGCCA
NaeI 93 GCCGGC GCC^GGC
847 GCCGGC
NgoMIV 93 GCCGGC G^CCGGC
847 GCCGGC
NspI 266 ACATGC [GA]CATG^[CT]
506 GCATGT
RcaI 344 TCATGA T^CATGA
395 TCATGA
SmaI 48 CCCGGG CCC^GGG
113 CCCGGG
XmaCI 48 CCCGGG C^CCGGG
113 CCCGGG
XmaI 48 CCCGGG C^CCGGG
113 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BanII BbeI BbsI BbvI
BcnI BmyI BpuAI BsaHI BsaJI
BsiYI BslI Bsp1286I BspMI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DsaI EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaII
HphI ItaI KasI MaeII MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIII NlaIV PvuII RsaI Sau96I
ScrFI SfoI TseI Tsp45I TspRI