Restriction map for
pF1KA1877
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AlwI AocI Aor51HI
AscI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MaeI MaeIII
MboI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI Psp1406I PvuI RsrII SacI
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AatII 1090 GACGTC GACGT^C
AcsI 1234 AAATTT [GA]^AATT[CT]
ApaI 296 GGGCCC GGGCC^C
ApoI 1234 AAATTT [GA]^AATT[CT]
AseI 1030 ATTAAT AT^TAAT
AsnI 1030 ATTAAT AT^TAAT
AviII 878 TGCGCA TGC^GCA
BglI 411 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 633 CGTCTC CGTCTC(1/5)
BsmI 32 GAATGC GAATGC(1/-1)
BspMI 284 ACCTGC ACCTGC(4/8)
BsrDI 18 GCAATG GCAATG(2/0)
BssSI 1405 CACGAG CACGAG(-5/-1)
BstAPI 699 GCACGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 700 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 660 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 926 CCTTGG C^C[AT][AT]GG
FspI 878 TGCGCA TGC^GCA
PpuMI 1109 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1030 ATTAAT AT^TAAT
SmaI 457 CCCGGG CCC^GGG
SspI 45 AATATT AAT^ATT
StyI 926 CCTTGG C^C[AT][AT]GG
XcmI 300 CCAGTCCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 457 CCCGGG C^CCGGG
XmaI 457 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 1351 GTGCAC G^TGCAC
1549 GTGCAC
ApaLI 1351 GTGCAC G^TGCAC
1549 GTGCAC
AspHI 1351 GTGCAC G[AT]GC[AT]^C
1549 GTGCAC
AvaI 457 CCCGGG C^[CT]CG[GA]G
1435 CTCGGG
AvaII 501 GGTCC G^G[AT]CC
1110 GGACC
BlpI 106 GCTGAGC GC^T[ACGT]AGC
735 GCTGAGC
BpmI 136 CTGGAG CTGGAG(16/14)
278 CTGGAG
Bpu1102I 106 GCTGAGC GC^T[ACGT]AGC
735 GCTGAGC
BsaBI 75 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1504 GATTTGCATC
BsaI 139 GAGACC GGTCTC(1/5)
281 GAGACC
BsaWI 530 ACCGGA [AT]^CCGG[AT]
1155 ACCGGA
BsiHKAI 1351 GTGCAC G[AT]GC[AT]^C
1549 GTGCAC
BsoBI 457 CCCGGG C^[CT]CG[GA]G
1435 CTCGGG
BspHI 948 TCATGA T^CATGA
1328 TCATGA
BsrI 300 CCAGT ACTGG(1/-1)
1570 CCAGT
BssHII 773 GCGCGC G^CGCGC
775 GCGCGC
CelII 106 GCTGAGC GC^T[ACGT]AGC
735 GCTGAGC
HphI 516 GGTGA GGTGA(8/7)
89 TCACC
KspI 714 CCGCGG CCGC^GG
870 CCGCGG
MamI 75 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1504 GATTTGCATC
PflMI 549 CCACGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1300 CCATCTTCTGG
RcaI 948 TCATGA T^CATGA
1328 TCATGA
SacII 714 CCGCGG CCGC^GG
870 CCGCGG
Tsp509I 1033 AATT ^AATT
1235 AATT
TspRI 1079 CACTG CA[GC]TG[ACGT][ACGT]^
1552 CACTG
Van91I 549 CCACGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1300 CCATCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI BanI BanII BbeI
BbrPI BbvI BcnI BmyI BsaAI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI Ksp632I MaeII
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV PmaCI PmlI
PstI PvuII RsaI Sau96I ScrFI
SfaNI SfcI SfoI TfiI Tru9I
TseI