Restriction map for
pF1KB6139
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BamHI BbrPI BcgI
BfrI BglII BlnI BsaAI BsaBI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI EarI EclXI Eco47III Eco52I
EcoRI FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspI StuI SwaI Tru9I
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcsI 38 AAATTC [GA]^AATT[CT]
AhdI 712 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 918 CCTGAGG CC^T[ACGT]AGG
ApoI 38 AAATTC [GA]^AATT[CT]
AspEI 712 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 113 TGCGCA TGC^GCA
BbeI 3 GGCGCC GGCGC^C
BbvCI 154 GCTGAGG CCTCAGC(-5/-2)
BciVI 1495 GTATCC GTATCC(6/5)
BclI 260 TGATCA T^GATCA
BsaI 799 GAGACC GGTCTC(1/5)
BsaWI 1170 TCCGGT [AT]^CCGG[AT]
BsmAI 799 GAGAC GTCTC(1/5)
Bsp1407I 1290 TGTACA T^GTACA
BsrDI 1767 CATTGC GCAATG(2/0)
BsrGI 1290 TGTACA T^GTACA
BstEII 1656 GGTGACC G^GT[ACGT]ACC
BstPI 1656 GGTGACC G^GT[ACGT]ACC
BstXI 1370 CCAGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 918 CCTGAGG CC^T[ACGT]AGG
DraIII 531 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 712 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 918 CCTGAGG CC^T[ACGT]AGG
EcoNI 150 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1656 GGTGACC G^GT[ACGT]ACC
EcoRV 145 GATATC GAT^ATC
EcoT22I 880 ATGCAT ATGCA^T
FbaI 260 TGATCA T^GATCA
FspI 113 TGCGCA TGC^GCA
HaeII 3 GGCGCC [GA]GCGC^[CT]
KasI 3 GGCGCC G^GCGCC
MslI 1282 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1373 GCCGGC GCC^GGC
NarI 3 GGCGCC GG^CGCC
NgoMIV 1373 GCCGGC G^CCGGC
NsiI 880 ATGCAT ATGCA^T
PaeR7I 505 CTCGAG C^TCGAG
PflMI 747 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1150 GACTC GAGTC(4/5)
PshAI 1425 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 547 CTGCAG CTGCA^G
SbfI 546 CCTGCAGG CCTGCA^GG
SfcI 547 CTGCAG C^T[GA][CT]AG
SfoI 3 GGCGCC GGC^GCC
SmlI 505 CTCGAG C^T[CT][GA]AG
Sse8387I 546 CCTGCAGG CCTGCA^GG
SspBI 1290 TGTACA T^GTACA
TfiI 396 GATTC G^A[AT]TC
Tsp509I 39 AATT ^AATT
Van91I 747 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 200 CCATTCCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 505 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 3 GGCGCC G[GA]^CG[CT]C
1453 GACGCC
BanI 3 GGCGCC G^G[CT][GA]CC
1191 GGTGCC
BfaI 352 CTAG C^TAG
1045 CTAG
BlpI 228 GCTGAGC GC^T[ACGT]AGC
810 GCTGAGC
Bpu1102I 228 GCTGAGC GC^T[ACGT]AGC
810 GCTGAGC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
1453 GACGCC
BspMI 325 ACCTGC ACCTGC(4/8)
1037 ACCTGC
BsrFI 1373 GCCGGC [GA]^CCGG[CT]
1466 ACCGGC
BstYI 983 AGATCC [GA]^GATC[CT]
1208 AGATCC
CelII 228 GCTGAGC GC^T[ACGT]AGC
810 GCTGAGC
Cfr10I 1373 GCCGGC [GA]^CCGG[CT]
1466 ACCGGC
EcoT14I 752 CCATGG C^C[AT][AT]GG
1115 CCATGG
Hin1I 3 GGCGCC G[GA]^CG[CT]C
1453 GACGCC
HinfI 396 GATTC G^A[ACGT]TC
1150 GACTC
MaeI 352 CTAG C^TAG
1045 CTAG
MaeII 385 ACGT A^CGT
742 ACGT
MflI 983 AGATCC [GA]^GATC[CT]
1208 AGATCC
NcoI 752 CCATGG C^CATGG
1115 CCATGG
SacI 736 GAGCTC GAGCT^C
913 GAGCTC
SmaI 1351 CCCGGG CCC^GGG
1583 CCCGGG
SphI 110 GCATGC GCATG^C
1281 GCATGC
StyI 752 CCATGG C^C[AT][AT]GG
1115 CCATGG
TaqI 440 TCGA T^CGA
506 TCGA
TthHB8I 440 TCGA T^CGA
506 TCGA
XhoII 983 AGATCC [GA]^GATC[CT]
1208 AGATCC
XmaCI 1351 CCCGGG C^CCGGG
1583 CCCGGG
XmaI 1351 CCCGGG C^CCGGG
1583 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI Asp700I AspHI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI XmnI