Restriction map for
pF1KE0559
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsaHI BsaWI BseAI
BsiEI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 515 GGTACC G^GTACC
AflIII 677 ACACGT A^C[GA][CT]GT
AocI 269 CCTCAGG CC^T[ACGT]AGG
Aor51HI 821 AGCGCT AGC^GCT
ApaI 85 GGGCCC GGGCC^C
Asp718I 515 GGTACC G^GTACC
AspHI 736 GAGCTC G[AT]GC[AT]^C
BbrPI 523 CACGTG CAC^GTG
BbvCI 864 CCTCAGC CCTCAGC(-5/-2)
BcnI 213 CCGGG CC^[GC]GG
BfaI 199 CTAG C^TAG
BglI 300 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 543 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 543 GCTGAGC GC^T[ACGT]AGC
BseRI 649 GAGGAG GAGGAG(10/8)
BsgI 526 GTGCAG GTGCAG(16/14)
BsiHKAI 736 GAGCTC G[AT]GC[AT]^C
BsiWI 228 CGTACG C^GTACG
BspHI 764 TCATGA T^CATGA
BstYI 617 AGATCC [GA]^GATC[CT]
Bsu36I 269 CCTCAGG CC^T[ACGT]AGG
CelII 543 GCTGAGC GC^T[ACGT]AGC
DrdI 400 GACGTGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 571 CTCTTC CTCTTC(1/4)
Eco47III 821 AGCGCT AGC^GCT
Eco81I 269 CCTCAGG CC^T[ACGT]AGG
EcoT14I 656 CCATGG C^C[AT][AT]GG
HaeIII 86 GGCC GG^CC
HapII 213 CCGG C^CGG
HpaII 213 CCGG C^CGG
KpnI 515 GGTACC GGTAC^C
Ksp632I 571 CTCTTC CTCTTC(1/4)
MaeI 199 CTAG C^TAG
MflI 617 AGATCC [GA]^GATC[CT]
MslI 153 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 213 CCGG C^CGG
NciI 213 CCGGG CC^[GC]GG
NcoI 656 CCATGG C^CATGG
PmaCI 523 CACGTG CAC^GTG
PmlI 523 CACGTG CAC^GTG
RcaI 764 TCATGA T^CATGA
SacI 736 GAGCTC GAGCT^C
ScaI 65 AGTACT AGT^ACT
SmlI 244 CTTGAG C^T[CT][GA]AG
SnaBI 226 TACGTA TAC^GTA
StyI 656 CCATGG C^C[AT][AT]GG
TaqI 827 TCGA T^CGA
TfiI 609 GATTC G^A[AT]TC
Tsp45I 318 GTCAC ^GT[GC]AC
TthHB8I 827 TCGA T^CGA
XhoII 617 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 166 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
346 CAGACTCTG
BbsI 580 GAAGAC GAAGAC(2/6)
792 GAAGAC
BbvI 860 GCAGC GCAGC(8/12)
857 GCTGC
BpuAI 580 GAAGAC GAAGAC(2/6)
792 GAAGAC
BsaI 652 GAGACC GGTCTC(1/5)
408 GGTCTC
BspMI 119 ACCTGC ACCTGC(4/8)
323 ACCTGC
BsrBI 55 CCGCTC CCGCTC(-3/-3)
873 CCGCTC
Cac8I 26 GCCCGC GC[ACGT]^[ACGT]GC
853 GCAGGC
EcoNI 642 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
761 CCTTCATGAGG
HaeII 635 GGCGCT [GA]GCGC^[CT]
821 AGCGCT
HphI 159 GGTGA GGTGA(8/7)
803 TCACC
MaeIII 209 GTAAC ^GT[ACGT]AC
318 GTCAC
PflMI 165 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
426 CCACCGTATGG
PleI 282 GACTC GAGTC(4/5)
348 GACTC
PstI 91 CTGCAG CTGCA^G
858 CTGCAG
SfaNI 829 GATGC GCATC(5/9)
752 GCATC
SfcI 91 CTGCAG C^T[GA][CT]AG
858 CTGCAG
TseI 857 GCTGC G^C[AT]GC
860 GCAGC
TspRI 32 CACTG CA[GC]TG[ACGT][ACGT]^
80 CACTG
Van91I 165 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
426 CCACCGTATGG
XcmI 656 CCATGGACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
797 CCACCTTCACCGTGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BmyI BpmI BsaAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI HhaI
HinP1I HinfI ItaI MaeII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI