Restriction map for
pF1KB6531
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstUI
BstZ17I CfoI ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 132 GACGCC G[GA]^CG[CT]C
Alw44I 673 GTGCAC G^TGCAC
AlwI 825 GATCC GGATC(4/5)
AocI 355 CCTGAGG CC^T[ACGT]AGG
ApaLI 673 GTGCAC G^TGCAC
AvaI 695 CCCGGG C^[CT]CG[GA]G
AvaII 116 GGACC G^G[AT]CC
BlpI 948 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 948 GCTGAGC GC^T[ACGT]AGC
BsaHI 132 GACGCC G[GA]^CG[CT]C
BsaWI 366 TCCGGT [AT]^CCGG[AT]
BsmAI 451 GTCTC GTCTC(1/5)
BsmI 645 GAATGC GAATGC(1/-1)
BsoBI 695 CCCGGG C^[CT]CG[GA]G
BsrDI 704 GCAATG GCAATG(2/0)
BstBI 767 TTCGAA TT^CGAA
BstEII 195 GGTCACC G^GT[ACGT]ACC
BstPI 195 GGTCACC G^GT[ACGT]ACC
BstYI 824 AGATCC [GA]^GATC[CT]
Bsu36I 355 CCTGAGG CC^T[ACGT]AGG
CelII 948 GCTGAGC GC^T[ACGT]AGC
DrdI 540 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 355 CCTGAGG CC^T[ACGT]AGG
EcoNI 99 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 195 GGTCACC G^GT[ACGT]ACC
EcoRI 571 GAATTC G^AATTC
EcoT14I 431 CCATGG C^C[AT][AT]GG
HgaI 132 GACGC GACGC(5/10)
Hin1I 132 GACGCC G[GA]^CG[CT]C
MaeII 923 ACGT A^CGT
MflI 824 AGATCC [GA]^GATC[CT]
MslI 738 CATTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 431 CCATGG C^CATGG
NspI 296 GCATGC [GA]CATG^[CT]
NspV 767 TTCGAA TT^CGAA
PflMI 969 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 898 GAGCTC GAGCT^C
SfaNI 691 GATGC GCATC(5/9)
SfuI 767 TTCGAA TT^CGAA
SmaI 695 CCCGGG CCC^GGG
SmlI 1075 CTCAAG C^T[CT][GA]AG
SphI 296 GCATGC GCATG^C
StuI 914 AGGCCT AGG^CCT
StyI 431 CCATGG C^C[AT][AT]GG
TfiI 796 GATTC G^A[AT]TC
Van91I 969 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 824 AGATCC [GA]^GATC[CT]
XmaCI 695 CCCGGG C^CCGGG
XmaI 695 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 571 GAATTC [GA]^AATT[CT]
807 AAATTC
ApoI 571 GAATTC [GA]^AATT[CT]
807 AAATTC
BanI 681 GGTGCC G^G[CT][GA]CC
862 GGTGCC
BbsI 129 GAAGAC GAAGAC(2/6)
764 GTCTTC
BbvCI 457 CCTCAGC CCTCAGC(-5/-2)
262 GCTGAGG
BfaI 50 CTAG C^TAG
340 CTAG
BpmI 301 CTGGAG CTGGAG(16/14)
313 CTGGAG
BpuAI 129 GAAGAC GAAGAC(2/6)
764 GTCTTC
BsgI 216 CTGCAC GTGCAG(16/14)
505 GTGCAG
BsmFI 115 GGGAC GGGAC(10/14)
831 GTCCC
BsrFI 306 GCCGGC [GA]^CCGG[CT]
996 GCCGGC
BstAPI 382 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
675 GCACACGGTGC
Cfr10I 306 GCCGGC [GA]^CCGG[CT]
996 GCCGGC
DraIII 676 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
1002 CACTGTGTG
EarI 345 CTCTTC CTCTTC(1/4)
7 GAAGAG
Ksp632I 345 CTCTTC CTCTTC(1/4)
7 GAAGAG
MaeI 50 CTAG C^TAG
340 CTAG
MaeIII 196 GTCAC ^GT[ACGT]AC
538 GTGAC
NaeI 306 GCCGGC GCC^GGC
996 GCCGGC
NgoMIV 306 GCCGGC G^CCGGC
996 GCCGGC
Tsp45I 196 GTCAC ^GT[GC]AC
538 GTGAC
XcmI 729 CCACTTCTCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
781 CCAGGTTGCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TseI
Tsp509I TspRI TthHB8I