Restriction map for
pF1KB8643
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AccII AclI AcsI AflII
AgeI AhdI AocI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BclI BfrI BglII
BlnI BsaBI BsgI BsiEI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MseI
MunI MvnI NarI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1039 GACGTC GACGT^C
AccI 727 GTAGAC GT^[AC][GT]AC
AccIII 661 TCCGGA T^CCGGA
Alw44I 1207 GTGCAC G^TGCAC
AlwNI 1444 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 564 AGCGCT AGC^GCT
ApaLI 1207 GTGCAC G^TGCAC
Asp700I 21 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 70 CACGTG CAC^GTG
BbsI 994 GTCTTC GAAGAC(2/6)
BciVI 949 GGATAC GTATCC(6/5)
BglI 247 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 994 GTCTTC GAAGAC(2/6)
BsaI 735 GAGACC GGTCTC(1/5)
BseAI 661 TCCGGA T^CCGGA
BsmI 1118 GCATTC GAATGC(1/-1)
BspEI 661 TCCGGA T^CCGGA
BstAPI 457 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 709 GGTTACC G^GT[ACGT]ACC
BstPI 709 GGTTACC G^GT[ACGT]ACC
BstXI 685 CCAATGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 490 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1228 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1435 CTCTTC CTCTTC(1/4)
Eco47III 564 AGCGCT AGC^GCT
EcoO109I 490 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 709 GGTTACC G^GT[ACGT]ACC
HaeII 564 AGCGCT [GA]GCGC^[CT]
Ksp632I 1435 CTCTTC CTCTTC(1/4)
MroI 661 TCCGGA T^CCGGA
NaeI 616 GCCGGC GCC^GGC
NcoI 248 CCATGG C^CATGG
NdeI 921 CATATG CA^TATG
NgoMIV 616 GCCGGC G^CCGGC
PflMI 940 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 70 CACGTG CAC^GTG
PmlI 70 CACGTG CAC^GTG
PpuMI 490 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 607 CAGCTG CAG^CTG
SacI 238 GAGCTC GAGCT^C
SapI 1434 GCTCTTC GCTCTTC(1/4)
SmaI 54 CCCGGG CCC^GGG
SspI 767 AATATT AAT^ATT
Van91I 940 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 54 CCCGGG C^CCGGG
XmaI 54 CCCGGG C^CCGGG
XmnI 21 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 1039 GACGTC G[GA]^CG[CT]C
1528 GACGCC
AfaI 1301 GTAC GT^AC
1362 GTAC
AvaI 54 CCCGGG C^[CT]CG[GA]G
376 CCCGAG
BalI 2 TGGCCA TGG^CCA
245 TGGCCA
BanII 238 GAGCTC G[GA]GC[CT]^C
861 GAGCCC
BfaI 800 CTAG C^TAG
1405 CTAG
BlpI 560 GCTCAGC GC^T[ACGT]AGC
567 GCTGAGC
BpmI 748 CTCCAG CTGGAG(16/14)
1441 CTCCAG
Bpu1102I 560 GCTCAGC GC^T[ACGT]AGC
567 GCTGAGC
BsaAI 70 CACGTG [CT]AC^GT[GA]
1363 TACGTG
BsaHI 1039 GACGTC G[GA]^CG[CT]C
1528 GACGCC
BseRI 700 CTCCTC GAGGAG(10/8)
1021 GAGGAG
BsmBI 1041 CGTCTC CGTCTC(1/5)
286 GAGACG
BsoBI 54 CCCGGG C^[CT]CG[GA]G
376 CCCGAG
BsrFI 616 GCCGGC [GA]^CCGG[CT]
1211 ACCGGC
BssSI 1183 CTCGTG CACGAG(-5/-1)
1381 CTCGTG
BstYI 836 AGATCC [GA]^GATC[CT]
911 GGATCT
CelII 560 GCTCAGC GC^T[ACGT]AGC
567 GCTGAGC
Cfr10I 616 GCCGGC [GA]^CCGG[CT]
1211 ACCGGC
DsaI 131 CCACGG C^C[GA][CT]GG
248 CCATGG
EcoT14I 248 CCATGG C^C[AT][AT]GG
887 CCAAGG
FauI 718 CCCGC CCCGC(4/6)
1054 CCCGC
Hin1I 1039 GACGTC G[GA]^CG[CT]C
1528 GACGCC
MaeI 800 CTAG C^TAG
1405 CTAG
MflI 836 AGATCC [GA]^GATC[CT]
911 GGATCT
MluNI 2 TGGCCA TGG^CCA
245 TGGCCA
MscI 2 TGGCCA TGG^CCA
245 TGGCCA
RsaI 1301 GTAC GT^AC
1362 GTAC
SmlI 634 CTTGAG C^T[CT][GA]AG
1510 CTCAAG
StuI 581 AGGCCT AGG^CCT
1499 AGGCCT
StyI 248 CCATGG C^C[AT][AT]GG
887 CCAAGG
TaqI 593 TCGA T^CGA
1076 TCGA
Tsp509I 26 AATT ^AATT
641 AATT
TthHB8I 593 TCGA T^CGA
1076 TCGA
XhoII 836 AGATCC [GA]^GATC[CT]
911 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AflIII AluI AlwI AspHI
AvaII BanI BbvI BcnI BmyI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI TseI Tsp45I TspRI
XcmI