Restriction map for
pF1KB7214
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbsI BbvCI BbvI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tru9I TseI Tth111I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 255 CGCG CG^CG
AcyI 87 GGCGCC G[GA]^CG[CT]C
AocI 562 CCTCAGG CC^T[ACGT]AGG
ApaI 168 GGGCCC GGGCC^C
BbeI 87 GGCGCC GGCGC^C
BbrPI 336 CACGTG CAC^GTG
BsaAI 336 CACGTG [CT]AC^GT[GA]
BsaHI 87 GGCGCC G[GA]^CG[CT]C
BsaI 435 GGTCTC GGTCTC(1/5)
BsaWI 607 ACCGGA [AT]^CCGG[AT]
BsmAI 436 GTCTC GTCTC(1/5)
BsmFI 102 GTCCC GGGAC(10/14)
BspMI 279 GCAGGT ACCTGC(4/8)
BsrI 71 ACTGG ACTGG(1/-1)
BstBI 488 TTCGAA TT^CGAA
BstUI 255 CGCG CG^CG
Bsu36I 562 CCTCAGG CC^T[ACGT]AGG
CfoI 88 GCGC GCG^C
DpnII 400 GATC ^GATC
DrdI 829 GACACATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 219 TGGCCG [CT]^GGCC[GA]
Eco81I 562 CCTCAGG CC^T[ACGT]AGG
EcoNI 111 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 87 GGCGCC [GA]GCGC^[CT]
HgaI 253 GACGC GACGC(5/10)
HhaI 88 GCGC GCG^C
Hin1I 87 GGCGCC G[GA]^CG[CT]C
HinP1I 88 GCGC G^CGC
KasI 87 GGCGCC G^GCGCC
MboI 400 GATC ^GATC
MboII 654 TCTTC GAAGA(8/7)
MvnI 255 CGCG CG^CG
NarI 87 GGCGCC GG^CGCC
NdeII 400 GATC ^GATC
NspV 488 TTCGAA TT^CGAA
PflMI 593 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 205 GACTC GAGTC(4/5)
PmaCI 336 CACGTG CAC^GTG
PmlI 336 CACGTG CAC^GTG
PpuMI 156 AGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 400 GATC ^GATC
SexAI 592 ACCAGGT A^CC[AT]GGT
SfoI 87 GGCGCC GGC^GCC
SfuI 488 TTCGAA TT^CGAA
StuI 713 AGGCCT AGG^CCT
TaqI 489 TCGA T^CGA
TthHB8I 489 TCGA T^CGA
Van91I 593 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 155 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
594 CAGGTGCTG
Asp700I 649 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
776 GAAAGGATTC
AvaI 20 CCCGGG C^[CT]CG[GA]G
61 CCCGGG
BanI 87 GGCGCC G^G[CT][GA]CC
319 GGCACC
BanII 144 GAGCCC G[GA]GC[CT]^C
168 GGGCCC
BmyI 144 GAGCCC G[AGT]GC[ACT]^C
168 GGGCCC
BpmI 152 CTCCAG CTGGAG(16/14)
508 CTCCAG
BsoBI 20 CCCGGG C^[CT]CG[GA]G
61 CCCGGG
Bsp1286I 144 GAGCCC G[AGT]GC[ACT]^C
168 GGGCCC
BstEII 327 GGTCACC G^GT[ACGT]ACC
603 GGTGACC
BstPI 327 GGTCACC G^GT[ACGT]ACC
603 GGTGACC
EcoO65I 327 GGTCACC G^GT[ACGT]ACC
603 GGTGACC
FauI 76 GCGGG CCCGC(4/6)
166 GCGGG
MaeII 190 ACGT A^CGT
337 ACGT
SmaI 20 CCCGGG CCC^GGG
61 CCCGGG
XmaCI 20 CCCGGG C^CCGGG
61 CCCGGG
XmaI 20 CCCGGG C^CCGGG
61 CCCGGG
XmnI 649 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
776 GAAAGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BcnI BsaJI BseRI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MnlI MslI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI TfiI
Tsp45I Tsp509I TspRI