Restriction map for
pF1KE0115
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BanII BbeI BbvCI
BcgI BciVI BclI BcnI BfaI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HaeIII HapII HgaI Hin1I
HincII HindII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MaeI
MamI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Asp700I 100 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 319 GGACC G^G[AT]CC
BamHI 81 GGATCC G^GATCC
BbrPI 507 CACGTG CAC^GTG
BbsI 590 GTCTTC GAAGAC(2/6)
BbvI 16 GCTGC GCAGC(8/12)
BpmI 311 CTCCAG CTGGAG(16/14)
BpuAI 590 GTCTTC GAAGAC(2/6)
BsaAI 507 CACGTG [CT]AC^GT[GA]
BsaJI 596 CCTTGG C^C[ACGT][ACGT]GG
BsiYI 465 CCTACAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 465 CCTACAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I 566 ACATGT A^CATGT
BsrI 420 ACTGG ACTGG(1/-1)
BstF5I 219 GGATG GGATG(2/0)
BstXI 506 CCACGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 319 GGACC G^G[ACGT]CC
DdeI 646 CTAAG C^T[ACGT]AG
DraI 330 TTTAAA TTT^AAA
EcoNI 465 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 596 CCTTGG C^C[AT][AT]GG
FauI 22 GCGGG CCCGC(4/6)
FokI 219 GGATG GGATG(9/13)
HindIII 438 AAGCTT A^AGCTT
MaeII 508 ACGT A^CGT
MaeIII 570 GTTAC ^GT[ACGT]AC
MfeI 9 CAATTG C^AATTG
MslI 370 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 314 CAGCTG C[AC]G^C[GT]G
MunI 9 CAATTG C^AATTG
MwoI 22 GCGGGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 567 CATG CATG^
NlaIV 81 GGATCC GG[ACGT]^[ACGT]CC
NspI 566 ACATGT [GA]CATG^[CT]
PmaCI 507 CACGTG CAC^GTG
PmlI 507 CACGTG CAC^GTG
PvuII 314 CAGCTG CAG^CTG
Sau96I 319 GGACC G^G[ACGT]CC
ScaI 357 AGTACT AGT^ACT
SfaNI 220 GATGC GCATC(5/9)
StyI 596 CCTTGG C^C[AT][AT]GG
TseI 16 GCTGC G^C[AT]GC
TspRI 641 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 100 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 19 GCGG CCGC(-3/-1)
22 GCGG
AcsI 126 AAATTT [GA]^AATT[CT]
522 GAATTT
AflII 441 CTTAAG C^TTAAG
582 CTTAAG
AflIII 508 ACGTGT A^C[GA][CT]GT
566 ACATGT
ApoI 126 AAATTT [GA]^AATT[CT]
522 GAATTT
BfrI 441 CTTAAG C^TTAAG
582 CTTAAG
BssKI 111 CCTGG ^CC[ACGT]GG
136 CCAGG
BstNI 111 CCTGG CC^[AT]GG
136 CCAGG
BstYI 81 GGATCC [GA]^GATC[CT]
611 AGATCC
Cac8I 22 GCGGGC GC[ACGT]^[ACGT]GC
436 GCAAGC
CfoI 14 GCGC GCG^C
120 GCGC
EcoRII 111 CCTGG ^CC[AT]GG
136 CCAGG
Fnu4HI 16 GCTGC GC^[ACGT]GC
19 GCGGC
HhaI 14 GCGC GCG^C
120 GCGC
HinP1I 14 GCGC G^CGC
120 GCGC
HinfI 282 GAATC G^A[ACGT]TC
387 GAATC
ItaI 16 GCTGC GC^[ACGT]GC
19 GCGGC
MflI 81 GGATCC [GA]^GATC[CT]
611 AGATCC
MvaI 111 CCTGG CC^[AT]GG
136 CCAGG
ScrFI 111 CCTGG CC^[ACGT]GG
136 CCAGG
TaqI 204 TCGA T^CGA
489 TCGA
TfiI 282 GAATC G^A[AT]TC
387 GAATC
TthHB8I 204 TCGA T^CGA
489 TCGA
XhoII 81 GGATCC [GA]^GATC[CT]
611 AGATCC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AfaI AluI AlwI DpnII MboI
MboII MnlI MseI NdeII RsaI
Sau3AI SmlI Tru9I Tsp509I