Restriction map for
pF1KE0154
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AvrII BamHI BanII
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoT22I FbaI
FseI HgaI HincII HindII HindIII
HpaI HphI KspI MamI MflI
MluI MroI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 65 GGTACC G^GTACC
AccII 297 CGCG CG^CG
AcyI 7 GGCGCC G[GA]^CG[CT]C
Asp718I 65 GGTACC G^GTACC
AspHI 233 GTGCTC G[AT]GC[AT]^C
AviII 258 TGCGCA TGC^GCA
BalI 38 TGGCCA TGG^CCA
BbeI 7 GGCGCC GGCGC^C
BbrPI 382 CACGTG CAC^GTG
BbsI 886 GAAGAC GAAGAC(2/6)
BbvCI 516 CCTCAGC CCTCAGC(-5/-2)
BcnI 803 CCCGG CC^[GC]GG
BpuAI 886 GAAGAC GAAGAC(2/6)
BsaHI 7 GGCGCC G[GA]^CG[CT]C
BsiEI 174 CGGCCG CG[GA][CT]^CG
BsiHKAI 233 GTGCTC G[AT]GC[AT]^C
BsmAI 60 GAGAC GTCTC(1/5)
BsmBI 60 GAGACG CGTCTC(1/5)
BspHI 374 TCATGA T^CATGA
BspMI 136 ACCTGC ACCTGC(4/8)
BstUI 297 CGCG CG^CG
Cac8I 521 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 294 GATC ^GATC
DrdI 469 GACGGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 86 CCATGG C^C[GA][CT]GG
EagI 174 CGGCCG C^GGCCG
EclXI 174 CGGCCG C^GGCCG
Eco52I 174 CGGCCG C^GGCCG
EcoRI 667 GAATTC G^AATTC
EcoRV 838 GATATC GAT^ATC
FauI 423 GCGGG CCCGC(4/6)
FspI 258 TGCGCA TGC^GCA
HaeII 7 GGCGCC [GA]GCGC^[CT]
Hin1I 7 GGCGCC G[GA]^CG[CT]C
KasI 7 GGCGCC G^GCGCC
KpnI 65 GGTACC GGTAC^C
MboI 294 GATC ^GATC
MfeI 345 CAATTG C^AATTG
MluNI 38 TGGCCA TGG^CCA
MscI 38 TGGCCA TGG^CCA
MunI 345 CAATTG C^AATTG
MvnI 297 CGCG CG^CG
MwoI 82 GCGACCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 7 GGCGCC GG^CGCC
NciI 803 CCCGG CC^[GC]GG
NcoI 86 CCATGG C^CATGG
NdeII 294 GATC ^GATC
PmaCI 382 CACGTG CAC^GTG
PmlI 382 CACGTG CAC^GTG
RcaI 374 TCATGA T^CATGA
SapI 359 GAAGAGC GCTCTTC(1/4)
Sau3AI 294 GATC ^GATC
SfaNI 850 GATGC GCATC(5/9)
SfoI 7 GGCGCC GGC^GCC
XbaI 879 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 180 GAATTT [GA]^AATT[CT]
667 GAATTC
ApoI 180 GAATTT [GA]^AATT[CT]
667 GAATTC
AvaII 438 GGACC G^G[AT]CC
806 GGACC
BanI 7 GGCGCC G^G[CT][GA]CC
65 GGTACC
BciVI 453 GTATCC GTATCC(6/5)
486 GTATCC
BfaI 280 CTAG C^TAG
880 CTAG
BpmI 238 CTCCAG CTGGAG(16/14)
402 CTCCAG
BsaAI 109 TACGTG [CT]AC^GT[GA]
382 CACGTG
BsiYI 69 CCTGAGATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
803 CCCGGACCAGG
BslI 69 CCTGAGATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
803 CCCGGACCAGG
BsmFI 116 GGGAC GGGAC(10/14)
712 GGGAC
CfoI 8 GCGC GCG^C
259 GCGC
Cfr13I 438 GGACC G^G[ACGT]CC
806 GGACC
DraI 352 TTTAAA TTT^AAA
504 TTTAAA
EaeI 38 TGGCCA [CT]^GGCC[GA]
174 CGGCCG
EarI 359 GAAGAG CTCTTC(1/4)
473 GAAGAG
HapII 177 CCGG C^CGG
804 CCGG
HhaI 8 GCGC GCG^C
259 GCGC
HinP1I 8 GCGC G^CGC
259 GCGC
HpaII 177 CCGG C^CGG
804 CCGG
Ksp632I 359 GAAGAG CTCTTC(1/4)
473 GAAGAG
MaeI 280 CTAG C^TAG
880 CTAG
MaeII 110 ACGT A^CGT
383 ACGT
MaeIII 539 GTCAC ^GT[ACGT]AC
799 GTGAC
MspA1I 241 CAGCTG C[AC]G^C[GT]G
519 CAGCTG
MspI 177 CCGG C^CGG
804 CCGG
NlaIII 87 CATG CATG^
375 CATG
NlaIV 7 GGCGCC GG[ACGT]^[ACGT]CC
65 GGTACC
PleI 378 GACTC GAGTC(4/5)
476 GAGTC
PvuII 241 CAGCTG CAG^CTG
519 CAGCTG
Sau96I 438 GGACC G^G[ACGT]CC
806 GGACC
TaqI 104 TCGA T^CGA
122 TCGA
TfiI 31 GATTC G^A[AT]TC
876 GATTC
Tsp45I 539 GTCAC ^GT[GC]AC
799 GTGAC
TthHB8I 104 TCGA T^CGA
122 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BbvI BmyI
BsaJI Bsp1286I BsrI BssKI BstF5I
BstNI DdeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MboII
MnlI MseI MvaI RsaI ScrFI
StyI Tru9I TseI Tsp509I TspRI