Restriction map for
pF1KB7651
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsgI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SbfI ScaI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Alw44I 892 GTGCAC G^TGCAC
AlwI 69 GATCC GGATC(4/5)
ApaLI 892 GTGCAC G^TGCAC
Asp700I 651 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 30 CCCGGG C^[CT]CG[GA]G
AviII 726 TGCGCA TGC^GCA
BfaI 959 CTAG C^TAG
BglII 635 AGATCT A^GATCT
BsaWI 584 ACCGGA [AT]^CCGG[AT]
BseRI 206 GAGGAG GAGGAG(10/8)
BsmAI 768 GAGAC GTCTC(1/5)
BsmBI 768 GAGACG CGTCTC(1/5)
BsoBI 30 CCCGGG C^[CT]CG[GA]G
BspMI 729 GCAGGT ACCTGC(4/8)
BssHII 690 GCGCGC G^CGCGC
BstAPI 918 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 568 GGTCACC G^GT[ACGT]ACC
BstPI 568 GGTCACC G^GT[ACGT]ACC
DdeI 376 CTCAG C^T[ACGT]AG
EcoNI 699 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 568 GGTCACC G^GT[ACGT]ACC
FspI 726 TGCGCA TGC^GCA
HincII 496 GTCAAC GT[CT]^[GA]AC
HindII 496 GTCAAC GT[CT]^[GA]AC
KspI 185 CCGCGG CCGC^GG
MaeI 959 CTAG C^TAG
MslI 966 CACGTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 402 GCCGGC GCC^GGC
NgoMIV 402 GCCGGC G^CCGGC
NotI 187 GCGGCCGC GC^GGCCGC
PflMI 441 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 943 GACTC GAGTC(4/5)
SacII 185 CCGCGG CCGC^GG
SexAI 739 ACCTGGT A^CC[AT]GGT
SfiI 241 GGCCCCGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 30 CCCGGG CCC^GGG
StuI 842 AGGCCT AGG^CCT
TfiI 921 GAATC G^A[AT]TC
TspRI 507 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 441 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
XmnI 651 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AfaI 224 GTAC GT^AC
672 GTAC
AluI 52 AGCT AG^CT
644 AGCT
Aor51HI 379 AGCGCT AGC^GCT
653 AGCGCT
AspHI 4 GAGCAC G[AT]GC[AT]^C
892 GTGCAC
BalI 449 TGGCCA TGG^CCA
701 TGGCCA
BanII 43 GAGCCC G[GA]GC[CT]^C
180 GGGCTC
BbeI 157 GGCGCC GGCGC^C
268 GGCGCC
BsiEI 188 CGGCCG CG[GA][CT]^CG
683 CGGCCG
BsiHKAI 4 GAGCAC G[AT]GC[AT]^C
892 GTGCAC
BsmFI 102 GGGAC GGGAC(10/14)
233 GTCCC
BsrBI 415 CCGCTC CCGCTC(-3/-3)
209 GAGCGG
BsrI 226 ACTGG ACTGG(1/-1)
508 ACTGG
BstF5I 819 CATCC GGATG(2/0)
527 GGATG
BstYI 68 AGATCC [GA]^GATC[CT]
635 AGATCT
DpnII 69 GATC ^GATC
636 GATC
EagI 188 CGGCCG C^GGCCG
683 CGGCCG
EarI 907 CTCTTC CTCTTC(1/4)
852 GAAGAG
EclXI 188 CGGCCG C^GGCCG
683 CGGCCG
Eco47III 379 AGCGCT AGC^GCT
653 AGCGCT
Eco52I 188 CGGCCG C^GGCCG
683 CGGCCG
FokI 819 CATCC GGATG(9/13)
527 GGATG
HinfI 921 GAATC G^A[ACGT]TC
943 GACTC
HphI 515 TCACC GGTGA(8/7)
570 TCACC
KasI 157 GGCGCC G^GCGCC
268 GGCGCC
Ksp632I 907 CTCTTC CTCTTC(1/4)
852 GAAGAG
MboI 69 GATC ^GATC
636 GATC
MflI 68 AGATCC [GA]^GATC[CT]
635 AGATCT
MluNI 449 TGGCCA TGG^CCA
701 TGGCCA
MscI 449 TGGCCA TGG^CCA
701 TGGCCA
NarI 157 GGCGCC GG^CGCC
268 GGCGCC
NdeII 69 GATC ^GATC
636 GATC
RsaI 224 GTAC GT^AC
672 GTAC
SapI 852 GAAGAGC GCTCTTC(1/4)
906 GCTCTTC
Sau3AI 69 GATC ^GATC
636 GATC
SfoI 157 GGCGCC GGC^GCC
268 GGCGCC
TaqI 62 TCGA T^CGA
946 TCGA
TthHB8I 62 TCGA T^CGA
946 TCGA
XhoII 68 AGATCC [GA]^GATC[CT]
635 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AlwNI AvaII
BanI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BsiYI BslI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI MaeII MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PpuMI PstI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I