Restriction map for
pF1KA0255
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AclI AflII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AvrII BamHI BbrPI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaI BsiEI BsiWI BsmBI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FauI FbaI FseI
HgaI HindIII KspI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1339 GTCTAC GT^[AC][GT]AC
AccII 36 CGCG CG^CG
AccIII 863 TCCGGA T^CCGGA
AcsI 1659 AAATTT [GA]^AATT[CT]
AocI 637 CCTGAGG CC^T[ACGT]AGG
ApoI 1659 AAATTT [GA]^AATT[CT]
AvaI 1420 CCCGAG C^[CT]CG[GA]G
AviII 1391 TGCGCA TGC^GCA
BalI 368 TGGCCA TGG^CCA
BbeI 1483 GGCGCC GGCGC^C
BbvCI 978 CCTCAGC CCTCAGC(-5/-2)
BfaI 1666 CTAG C^TAG
BsaAI 549 CACGTA [CT]AC^GT[GA]
BsaBI 1020 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 863 TCCGGA T^CCGGA
BsoBI 1420 CCCGAG C^[CT]CG[GA]G
BspEI 863 TCCGGA T^CCGGA
BspLU11I 1835 ACATGT A^CATGT
BsrFI 449 ACCGGC [GA]^CCGG[CT]
BssSI 307 CTCGTG CACGAG(-5/-1)
BstUI 36 CGCG CG^CG
Bsu36I 637 CCTGAGG CC^T[ACGT]AGG
Cfr10I 449 ACCGGC [GA]^CCGG[CT]
DraI 1158 TTTAAA TTT^AAA
Eco81I 637 CCTGAGG CC^T[ACGT]AGG
EcoNI 861 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 727 GATATC GAT^ATC
FspI 1391 TGCGCA TGC^GCA
HpaI 1720 GTTAAC GTT^AAC
KasI 1483 GGCGCC G^GCGCC
MaeI 1666 CTAG C^TAG
MamI 1020 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 368 TGGCCA TGG^CCA
MroI 863 TCCGGA T^CCGGA
MscI 368 TGGCCA TGG^CCA
MvnI 36 CGCG CG^CG
NarI 1483 GGCGCC GG^CGCC
NdeI 1376 CATATG CA^TATG
PpuMI 599 AGGACCT [GA]G^G[AT]CC[CT]
SapI 1500 GCTCTTC GCTCTTC(1/4)
SfoI 1483 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 772 GACGTC GACGT^C
946 GACGTC
Acc65I 643 GGTACC G^GTACC
1810 GGTACC
AflIII 548 ACACGT A^C[GA][CT]GT
1835 ACATGT
Alw44I 627 GTGCAC G^TGCAC
937 GTGCAC
ApaLI 627 GTGCAC G^TGCAC
937 GTGCAC
Asp700I 1242 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1530 GAATCAGTTC
Asp718I 643 GGTACC G^GTACC
1810 GGTACC
BcgI 1226 GCATCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1310 GCATGTGGTTCG
BciVI 833 GTATCC GTATCC(6/5)
1585 GTATCC
BglII 476 AGATCT A^GATCT
1850 AGATCT
BsaWI 197 ACCGGA [AT]^CCGG[AT]
863 TCCGGA
BsmAI 398 GAGAC GTCTC(1/5)
1669 GTCTC
BsmFI 194 GGGAC GGGAC(10/14)
749 GGGAC
BsmI 1001 GCATTC GAATGC(1/-1)
1684 GCATTC
BspHI 227 TCATGA T^CATGA
1082 TCATGA
BspMI 359 ACCTGC ACCTGC(4/8)
482 ACCTGC
BsrBI 316 GAGCGG CCGCTC(-3/-3)
1274 GAGCGG
BsrDI 348 CATTGC GCAATG(2/0)
873 CATTGC
BstXI 371 CCACCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1289 CCACCATGGTGG
DraII 599 AGGACCT [GA]G^G[ACGT]CC[CT]
955 AGGCCCC
DrdI 196 GACCGGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
331 GACTACTACGTC
DsaI 911 CCATGG C^C[GA][CT]GG
1292 CCATGG
EcoO109I 599 AGGACCT [GA]G^G[ACGT]CC[CT]
955 AGGCCCC
HaeII 13 AGCGCC [GA]GCGC^[CT]
1483 GGCGCC
KpnI 643 GGTACC GGTAC^C
1810 GGTACC
MaeIII 280 GTGAC ^GT[ACGT]AC
770 GTGAC
MwoI 140 GCCAGCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1047 GCTTGGGATGC
NcoI 911 CCATGG C^CATGG
1292 CCATGG
NspI 1310 GCATGT [GA]CATG^[CT]
1835 ACATGT
PleI 305 GACTC GAGTC(4/5)
919 GAGTC
RcaI 227 TCATGA T^CATGA
1082 TCATGA
Tsp45I 280 GTGAC ^GT[GC]AC
770 GTGAC
XmnI 1242 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1530 GAATCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI NciI NdeII NlaIII NlaIV
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII