Restriction map for
pF1KE0490
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI BbrPI
BbvCI BciVI BclI BfrI BsaBI
BsaWI BseAI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 616 GACGTC GACGT^C
AcsI 1216 GAATTC [GA]^AATT[CT]
AflIII 323 ACGTGT A^C[GA][CT]GT
Aor51HI 233 AGCGCT AGC^GCT
ApaI 1117 GGGCCC GGGCC^C
ApoI 1216 GAATTC [GA]^AATT[CT]
AvaII 576 GGTCC G^G[AT]CC
AviII 245 TGCGCA TGC^GCA
AvrII 399 CCTAGG C^CTAGG
BalI 797 TGGCCA TGG^CCA
BamHI 30 GGATCC G^GATCC
BanII 1117 GGGCCC G[GA]GC[CT]^C
BbsI 1188 GAAGAC GAAGAC(2/6)
BcgI 52 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 400 CTAG C^TAG
BglII 191 AGATCT A^GATCT
BlnI 399 CCTAGG C^CTAGG
BpuAI 1188 GAAGAC GAAGAC(2/6)
BsaI 1024 GAGACC GGTCTC(1/5)
BsgI 1048 GTGCAG GTGCAG(16/14)
BsiEI 47 CGACCG CG[GA][CT]^CG
BsmBI 69 CGTCTC CGTCTC(1/5)
BsmFI 608 GGGAC GGGAC(10/14)
BsrFI 874 GCCGGC [GA]^CCGG[CT]
BstXI 95 CCACTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 874 GCCGGC [GA]^CCGG[CT]
DrdI 610 GACTTTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 797 TGGCCA [CT]^GGCC[GA]
Eco47III 233 AGCGCT AGC^GCT
EcoRI 1216 GAATTC G^AATTC
FspI 245 TGCGCA TGC^GCA
MaeI 400 CTAG C^TAG
MluNI 797 TGGCCA TGG^CCA
MscI 797 TGGCCA TGG^CCA
NaeI 874 GCCGGC GCC^GGC
NcoI 101 CCATGG C^CATGG
NgoMIV 874 GCCGGC G^CCGGC
PstI 532 CTGCAG CTGCA^G
SapI 999 GAAGAGC GCTCTTC(1/4)
ScaI 317 AGTACT AGT^ACT
SmaI 707 CCCGGG CCC^GGG
SphI 788 GCATGC GCATG^C
XcmI 1158 CCACAGCCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 707 CCCGGG C^CCGGG
XmaI 707 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 82 CCCGAG C^[CT]CG[GA]G
707 CCCGGG
BbeI 1135 GGCGCC GGCGC^C
1171 GGCGCC
BsaAI 322 TACGTG [CT]AC^GT[GA]
637 TACGTG
BseRI 295 CTCCTC GAGGAG(10/8)
404 GAGGAG
BsiYI 600 CCGGGATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
843 CCGCGTGTCGG
BslI 600 CCGGGATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
843 CCGCGTGTCGG
BsmAI 1024 GAGAC GTCTC(1/5)
70 GTCTC
BsoBI 82 CCCGAG C^[CT]CG[GA]G
707 CCCGGG
BsrBI 134 CCGCTC CCGCTC(-3/-3)
1152 CCGCTC
BsrI 315 CCAGT ACTGG(1/-1)
623 CCAGT
BstAPI 467 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
783 GCAAGGCATGC
BstYI 30 GGATCC [GA]^GATC[CT]
191 AGATCT
KasI 1135 GGCGCC G^GCGCC
1171 GGCGCC
MaeIII 619 GTCAC ^GT[ACGT]AC
640 GTGAC
MflI 30 GGATCC [GA]^GATC[CT]
191 AGATCT
MslI 96 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
894 CATCTGTGTG
NarI 1135 GGCGCC GG^CGCC
1171 GGCGCC
PvuII 216 CAGCTG CAG^CTG
453 CAGCTG
SfaNI 248 GCATC GCATC(5/9)
1004 GCATC
SfoI 1135 GGCGCC GGC^GCC
1171 GGCGCC
SmlI 739 CTCAAG C^T[CT][GA]AG
1018 CTCAAG
StuI 356 AGGCCT AGG^CCT
584 AGGCCT
Tsp45I 619 GTCAC ^GT[GC]AC
640 GTGAC
Tsp509I 373 AATT ^AATT
1217 AATT
XhoII 30 GGATCC [GA]^GATC[CT]
191 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI BanI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI Bsp1286I BspMI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfcI StyI TseI TspRI