################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BanI BbeI BbrPI BcgI BciVI BfrI BglI BglII BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AvrII 1236 CCTAGG C^CTAGG BamHI 1510 GGATCC G^GATCC BanII 1825 GAGCCC G[GA]GC[CT]^C BbvCI 97 CCTCAGC CCTCAGC(-5/-2) BcnI 1619 CCCGG CC^[GC]GG BlnI 1236 CCTAGG C^CTAGG BlpI 1816 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1816 GCTGAGC GC^T[ACGT]AGC BsaAI 2279 CACGTA [CT]AC^GT[GA] BsaBI 1463 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 408 ACCGGA [AT]^CCGG[AT] BsmI 1164 GAATGC GAATGC(1/-1) Bsp1407I 2340 TGTACA T^GTACA BspLU11I 2378 ACATGT A^CATGT BsrBI 211 GAGCGG CCGCTC(-3/-3) BsrGI 2340 TGTACA T^GTACA CelII 1816 GCTGAGC GC^T[ACGT]AGC CfoI 1504 GCGC GCG^C DrdI 2176 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1530 TGGCCG [CT]^GGCC[GA] EcoRV 1228 GATATC GAT^ATC HhaI 1504 GCGC GCG^C HinP1I 1504 GCGC G^CGC HincII 885 GTTAAC GT[CT]^[GA]AC HindII 885 GTTAAC GT[CT]^[GA]AC HpaI 885 GTTAAC GTT^AAC MamI 1463 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 392 CAATTG C^AATTG MslI 2379 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 392 CAATTG C^AATTG NciI 1619 CCCGG CC^[GC]GG NcoI 1032 CCATGG C^CATGG PflMI 1032 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfcI 1472 CTACAG C^T[GA][CT]AG SspBI 2340 TGTACA T^GTACA StuI 1576 AGGCCT AGG^CCT Van91I 1032 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AatII 947 GACGTC GACGT^C 2098 GACGTC AspHI 534 GTGCTC G[AT]GC[AT]^C 1562 GTGCTC BclI 2300 TGATCA T^GATCA 2328 TGATCA BpmI 1133 CTCCAG CTGGAG(16/14) 1358 CTGGAG BseRI 249 CTCCTC GAGGAG(10/8) 1864 GAGGAG BsgI 1679 CTGCAC GTGCAG(16/14) 455 GTGCAG BsiHKAI 534 GTGCTC G[AT]GC[AT]^C 1562 GTGCTC DraI 381 TTTAAA TTT^AAA 1057 TTTAAA DsaI 1032 CCATGG C^C[GA][CT]GG 1933 CCACGG FbaI 2300 TGATCA T^GATCA 2328 TGATCA HgaI 1615 GCGTC GACGC(5/10) 1718 GCGTC HindIII 161 AAGCTT A^AGCTT 593 AAGCTT MspA1I 125 CAGCTG C[AC]G^C[GT]G 1640 CAGCTG PpuMI 2019 AGGACCT [GA]G^G[AT]CC[CT] 2333 AGGTCCT PshAI 1657 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1852 GACAAGAGTC PvuII 125 CAGCTG CAG^CTG 1640 CAGCTG SmlI 1159 CTTGAG C^T[CT][GA]AG 1538 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AvaII BbsI BbvI BfaI BmyI BpuAI BsaHI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII