Restriction map for
pF1KE0127
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspI AvaI
AvaII AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcnI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HpaI KasI Ksp632I KspI
MamI MfeI MflI MluI MroI
MspA1I MunI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 5 GGTACC G^GTACC
AciI 240 CCGC CCGC(-3/-1)
AcsI 862 GAATTT [GA]^AATT[CT]
AocI 856 CCTGAGG CC^T[ACGT]AGG
ApoI 862 GAATTT [GA]^AATT[CT]
Asp718I 5 GGTACC G^GTACC
AspHI 598 GAGCAC G[AT]GC[AT]^C
BalI 358 TGGCCA TGG^CCA
BanII 748 GAGCCC G[GA]GC[CT]^C
BcgI 229 CGAGACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 285 GGATAC GTATCC(6/5)
BclI 989 TGATCA T^GATCA
BlpI 242 GCTCAGC GC^T[ACGT]AGC
BpmI 681 CTCCAG CTGGAG(16/14)
Bpu1102I 242 GCTCAGC GC^T[ACGT]AGC
BsaJI 967 CCATGG C^C[ACGT][ACGT]GG
BsiHKAI 598 GAGCAC G[AT]GC[AT]^C
BsiYI 644 CCATTTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 644 CCATTTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 1089 CGTCTC CGTCTC(1/5)
BsrBI 240 CCGCTC CCGCTC(-3/-3)
BssKI 282 CCTGG ^CC[ACGT]GG
BstNI 282 CCTGG CC^[AT]GG
Bsu36I 856 CCTGAGG CC^T[ACGT]AGG
CelII 242 GCTCAGC GC^T[ACGT]AGC
DsaI 967 CCATGG C^C[GA][CT]GG
EaeI 358 TGGCCA [CT]^GGCC[GA]
Eco81I 856 CCTGAGG CC^T[ACGT]AGG
EcoRII 282 CCTGG ^CC[AT]GG
EcoT14I 967 CCATGG C^C[AT][AT]GG
FbaI 989 TGATCA T^GATCA
HapII 70 CCGG C^CGG
HincII 1021 GTCAAC GT[CT]^[GA]AC
HindII 1021 GTCAAC GT[CT]^[GA]AC
HindIII 96 AAGCTT A^AGCTT
HpaII 70 CCGG C^CGG
KpnI 5 GGTACC GGTAC^C
MaeII 218 ACGT A^CGT
MluNI 358 TGGCCA TGG^CCA
MscI 358 TGGCCA TGG^CCA
MspI 70 CCGG C^CGG
MvaI 282 CCTGG CC^[AT]GG
MwoI 885 GCCATTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 967 CCATGG C^CATGG
NheI 708 GCTAGC G^CTAGC
ScrFI 282 CCTGG CC^[ACGT]GG
SmlI 593 CTCAAG C^T[CT][GA]AG
SspI 1068 AATATT AAT^ATT
StyI 967 CCATGG C^C[AT][AT]GG
TaqI 228 TCGA T^CGA
TfiI 821 GATTC G^A[AT]TC
TthHB8I 228 TCGA T^CGA
XcmI 704 CCAAGCTAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AhdI 321 GACCATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1074 GACTTATCGTC
AlwI 623 GGATC GGATC(4/5)
717 GGATC
AspEI 321 GACCATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1074 GACTTATCGTC
BmyI 598 GAGCAC G[AGT]GC[ACT]^C
748 GAGCCC
BsaI 230 GAGACC GGTCTC(1/5)
337 GAGACC
Bsp1286I 598 GAGCAC G[AGT]GC[ACT]^C
748 GAGCCC
BstXI 323 CCATTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
930 CCACATGATTGG
CfoI 207 GCGC GCG^C
276 GCGC
Cfr13I 72 GGCCC G^G[ACGT]CC
946 GGGCC
Eam1105I 321 GACCATTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1074 GACTTATCGTC
HhaI 207 GCGC GCG^C
276 GCGC
HinP1I 207 GCGC G^CGC
276 GCGC
MslI 759 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1043 CACTCCAATG
PleI 267 GACTC GAGTC(4/5)
59 GAGTC
Sau96I 72 GGCCC G^G[ACGT]CC
946 GGGCC
SfaNI 204 GATGC GCATC(5/9)
247 GCATC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI BanI BbvI BfaI
BseRI BsmAI BsmFI BsmI BsrI
BstF5I Cac8I DdeI DpnII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI NdeII NlaIII NlaIV RsaI
Sau3AI Tru9I TseI Tsp45I Tsp509I
TspRI