Restriction map for
pF1KE0451
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoT22I FokI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AlwI 637 GGATC GGATC(4/5)
ApaI 647 GGGCCC GGGCC^C
Asp700I 679 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 453 CCTAGG C^CTAGG
BanI 21 GGCACC G^G[CT][GA]CC
BbsI 370 GTCTTC GAAGAC(2/6)
BclI 485 TGATCA T^GATCA
BcnI 220 CCGGG CC^[GC]GG
BlnI 453 CCTAGG C^CTAGG
BpmI 823 CTGGAG CTGGAG(16/14)
BpuAI 370 GTCTTC GAAGAC(2/6)
BsgI 97 CTGCAC GTGCAG(16/14)
Bsp1407I 609 TGTACA T^GTACA
BsrBI 349 GAGCGG CCGCTC(-3/-3)
BsrFI 554 ACCGGC [GA]^CCGG[CT]
BsrGI 609 TGTACA T^GTACA
BstEII 745 GGTTACC G^GT[ACGT]ACC
BstPI 745 GGTTACC G^GT[ACGT]ACC
Cfr10I 554 ACCGGC [GA]^CCGG[CT]
DraII 646 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 559 CGGCCA [CT]^GGCC[GA]
EarI 568 GAAGAG CTCTTC(1/4)
EcoO109I 646 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 745 GGTTACC G^GT[ACGT]ACC
EcoRV 224 GATATC GAT^ATC
FbaI 485 TGATCA T^GATCA
HgaI 378 GACGC GACGC(5/10)
HincII 154 GTCAAC GT[CT]^[GA]AC
HindII 154 GTCAAC GT[CT]^[GA]AC
Ksp632I 568 GAAGAG CTCTTC(1/4)
NciI 220 CCGGG CC^[GC]GG
PstI 505 CTGCAG CTGCA^G
ScaI 599 AGTACT AGT^ACT
SfcI 505 CTGCAG C^T[GA][CT]AG
SgrAI 553 CACCGGCG C[GA]^CCGG[CT]G
SspBI 609 TGTACA T^GTACA
TaqI 89 TCGA T^CGA
TfiI 893 GATTC G^A[AT]TC
Tsp45I 850 GTCAC ^GT[GC]AC
TthHB8I 89 TCGA T^CGA
XcmI 578 CCATCTTGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 679 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 600 GTAC GT^AC
610 GTAC
BanII 48 GAGCCC G[GA]GC[CT]^C
647 GGGCCC
BseRI 337 CTCCTC GAGGAG(10/8)
536 GAGGAG
BsmFI 31 GGGAC GGGAC(10/14)
266 GTCCC
Cac8I 215 GCTTGC GC[ACGT]^[ACGT]GC
384 GCAGGC
CfoI 187 GCGC GCG^C
885 GCGC
DpnII 486 GATC ^GATC
638 GATC
EcoT14I 453 CCTAGG C^C[AT][AT]GG
861 CCTTGG
HhaI 187 GCGC GCG^C
885 GCGC
HinP1I 187 GCGC G^CGC
885 GCGC
MboI 486 GATC ^GATC
638 GATC
NdeII 486 GATC ^GATC
638 GATC
PleI 756 GACTC GAGTC(4/5)
478 GAGTC
RsaI 600 GTAC GT^AC
610 GTAC
Sau3AI 486 GATC ^GATC
638 GATC
SfaNI 834 GATGC GCATC(5/9)
911 GCATC
StyI 453 CCTAGG C^C[AT][AT]GG
861 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI EcoRII
FauI Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboII MnlI MslI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI TspRI