Restriction map for
pF1KE2418
##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflIII
AhdI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BfaI BglI BglII BlnI BsaAI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BsrDI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI MaeI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SalI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 679 GACGTC GACGT^C
AflII 2140 CTTAAG C^TTAAG
AocI 327 CCTCAGG CC^T[ACGT]AGG
ApaI 1126 GGGCCC GGGCC^C
AspI 298 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1571 TGGCCA TGG^CCA
BbvCI 2479 GCTGAGG CCTCAGC(-5/-2)
BclI 1337 TGATCA T^GATCA
BfrI 2140 CTTAAG C^TTAAG
BlpI 2422 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2422 GCTCAGC GC^T[ACGT]AGC
BspHI 1700 TCATGA T^CATGA
BsrBI 2163 CCGCTC CCGCTC(-3/-3)
BssSI 193 CACGAG CACGAG(-5/-1)
BstEII 651 GGTGACC G^GT[ACGT]ACC
BstPI 651 GGTGACC G^GT[ACGT]ACC
Bsu36I 327 CCTCAGG CC^T[ACGT]AGG
CelII 2422 GCTCAGC GC^T[ACGT]AGC
DraIII 1110 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 327 CCTCAGG CC^T[ACGT]AGG
EcoO65I 651 GGTGACC G^GT[ACGT]ACC
FbaI 1337 TGATCA T^GATCA
HaeII 835 AGCGCC [GA]GCGC^[CT]
KspI 1668 CCGCGG CCGC^GG
MluNI 1571 TGGCCA TGG^CCA
MscI 1571 TGGCCA TGG^CCA
PaeR7I 2405 CTCGAG C^TCGAG
PflFI 298 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1700 TCATGA T^CATGA
SacI 982 GAGCTC GAGCT^C
SacII 1668 CCGCGG CCGC^GG
SgrAI 273 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2289 CCCGGG CCC^GGG
StuI 2156 AGGCCT AGG^CCT
Tth111I 298 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2405 CTCGAG C^TCGAG
XmaCI 2289 CCCGGG C^CCGGG
XmaI 2289 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AgeI 274 ACCGGT A^CCGGT
2216 ACCGGT
Alw44I 771 GTGCAC G^TGCAC
1132 GTGCAC
ApaLI 771 GTGCAC G^TGCAC
1132 GTGCAC
Asp700I 2130 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2154 GAAGGCCTTC
BanI 277 GGTGCC G^G[CT][GA]CC
1091 GGCACC
BsaBI 936 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1683 GATGGGCATC
BsaWI 274 ACCGGT [AT]^CCGG[AT]
2216 ACCGGT
BsmBI 263 GAGACG CGTCTC(1/5)
633 GAGACG
Bsp1407I 926 TGTACA T^GTACA
1331 TGTACA
BsrFI 274 ACCGGT [GA]^CCGG[CT]
2216 ACCGGT
BsrGI 926 TGTACA T^GTACA
1331 TGTACA
Cfr10I 274 ACCGGT [GA]^CCGG[CT]
2216 ACCGGT
DraII 1851 GGGGCCT [GA]G^G[ACGT]CC[CT]
1864 AGGCCCC
DrdI 1282 GACAGATTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2476 GACGCTGAGGTC
EcoO109I 1851 GGGGCCT [GA]G^G[ACGT]CC[CT]
1864 AGGCCCC
HincII 571 GTCAAC GT[CT]^[GA]AC
2059 GTCAAC
HindII 571 GTCAAC GT[CT]^[GA]AC
2059 GTCAAC
MamI 936 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1683 GATGGGCATC
PflMI 381 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1677 CCAGCAGATGG
PinAI 274 ACCGGT A^CCGGT
2216 ACCGGT
SbfI 1266 CCTGCAGG CCTGCA^GG
1859 CCTGCAGG
Sse8387I 1266 CCTGCAGG CCTGCA^GG
1859 CCTGCAGG
SspBI 926 TGTACA T^GTACA
1331 TGTACA
Tsp509I 1404 AATT ^AATT
2004 AATT
Van91I 381 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1677 CCAGCAGATGG
XcmI 839 CCAGGGACTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2108 CCATCACCATCCTGG
XmnI 2130 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2154 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII