Restriction map for
pF1KB7660
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AclI AfaI AflII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BpuAI BsaAI BsaBI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MseI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 712 GTAGAC GT^[AC][GT]AC
AccIII 934 TCCGGA T^CCGGA
AcsI 332 GAATTC [GA]^AATT[CT]
AflIII 271 ACGTGT A^C[GA][CT]GT
AgeI 598 ACCGGT A^CCGGT
AlwNI 587 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 332 GAATTC [GA]^AATT[CT]
AspHI 740 GTGCTC G[AT]GC[AT]^C
BamHI 84 GGATCC G^GATCC
BfaI 910 CTAG C^TAG
BglII 364 AGATCT A^GATCT
BlpI 1123 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1123 GCTCAGC GC^T[ACGT]AGC
BseAI 934 TCCGGA T^CCGGA
BsiEI 261 CGGTCG CG[GA][CT]^CG
BsiHKAI 740 GTGCTC G[AT]GC[AT]^C
BspEI 934 TCCGGA T^CCGGA
BspMI 759 GCAGGT ACCTGC(4/8)
BsrFI 598 ACCGGT [GA]^CCGG[CT]
BssHII 1095 GCGCGC G^CGCGC
BstEII 904 GGTCACC G^GT[ACGT]ACC
BstPI 904 GGTCACC G^GT[ACGT]ACC
BstXI 974 CCATGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1123 GCTCAGC GC^T[ACGT]AGC
Cfr10I 598 ACCGGT [GA]^CCGG[CT]
CpoI 1007 CGGTCCG CG^G[AT]CCG
EcoO65I 904 GGTCACC G^GT[ACGT]ACC
EcoRI 332 GAATTC G^AATTC
HgaI 41 GCGTC GACGC(5/10)
KspI 258 CCGCGG CCGC^GG
MaeI 910 CTAG C^TAG
MaeII 271 ACGT A^CGT
MroI 934 TCCGGA T^CCGGA
NcoI 974 CCATGG C^CATGG
NspI 716 ACATGC [GA]CATG^[CT]
PinAI 598 ACCGGT A^CCGGT
RsrII 1007 CGGTCCG CG^G[AT]CCG
SacII 258 CCGCGG CCGC^GG
SfaNI 814 GATGC GCATC(5/9)
SfcI 710 CTGTAG C^T[GA][CT]AG
SmaI 620 CCCGGG CCC^GGG
StuI 426 AGGCCT AGG^CCT
TfiI 373 GATTC G^A[AT]TC
XmaCI 620 CCCGGG C^CCGGG
XmaI 620 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 259 CGCG CG^CG
1096 CGCG
AcyI 352 GGCGCC G[GA]^CG[CT]C
406 GGCGCC
AocI 344 CCTCAGG CC^T[ACGT]AGG
481 CCTCAGG
AvaI 620 CCCGGG C^[CT]CG[GA]G
1137 CTCGGG
BbeI 352 GGCGCC GGCGC^C
406 GGCGCC
BglI 941 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1167 GCCCAATGGGC
BsaHI 352 GGCGCC G[GA]^CG[CT]C
406 GGCGCC
BsaI 293 GAGACC GGTCTC(1/5)
595 GAGACC
BsaWI 598 ACCGGT [AT]^CCGG[AT]
934 TCCGGA
BsoBI 620 CCCGGG C^[CT]CG[GA]G
1137 CTCGGG
BsrI 499 ACTGG ACTGG(1/-1)
507 ACTGG
BstUI 259 CGCG CG^CG
1096 CGCG
Bsu36I 344 CCTCAGG CC^T[ACGT]AGG
481 CCTCAGG
EarI 18 GAAGAG CTCTTC(1/4)
748 GAAGAG
Eco81I 344 CCTCAGG CC^T[ACGT]AGG
481 CCTCAGG
EcoNI 429 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
641 CCTCTCTGAGG
EcoT14I 536 CCAAGG C^C[AT][AT]GG
974 CCATGG
Hin1I 352 GGCGCC G[GA]^CG[CT]C
406 GGCGCC
KasI 352 GGCGCC G^GCGCC
406 GGCGCC
Ksp632I 18 GAAGAG CTCTTC(1/4)
748 GAAGAG
MaeIII 43 GTCAC ^GT[ACGT]AC
905 GTCAC
MvnI 259 CGCG CG^CG
1096 CGCG
NarI 352 GGCGCC GG^CGCC
406 GGCGCC
PleI 320 GACTC GAGTC(4/5)
775 GACTC
PpuMI 682 AGGACCC [GA]G^G[AT]CC[CT]
1142 GGGTCCC
SfoI 352 GGCGCC GGC^GCC
406 GGCGCC
StyI 536 CCAAGG C^C[AT][AT]GG
974 CCATGG
TaqI 336 TCGA T^CGA
965 TCGA
Tsp45I 43 GTCAC ^GT[GC]AC
905 GTCAC
Tsp509I 333 AATT ^AATT
1049 AATT
TthHB8I 336 TCGA T^CGA
965 TCGA
XcmI 377 CCAGGTTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
392 CCAGCCTCGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaII
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PvuII Sau3AI Sau96I ScrFI
TseI TspRI Van91I XhoII