Restriction map for
pF1KE0547
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsiWI BspDI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoT22I FseI FspI HpaI KpnI
MaeI MamI MfeI MluI MluNI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuII RsrII SacI SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AccII 817 CGCG CG^CG
AccIII 380 TCCGGA T^CCGGA
AcsI 102 GAATTT [GA]^AATT[CT]
AcyI 888 GGCGCC G[GA]^CG[CT]C
AocI 1111 CCTAAGG CC^T[ACGT]AGG
ApaI 1213 GGGCCC GGGCC^C
ApoI 102 GAATTT [GA]^AATT[CT]
AseI 1042 ATTAAT AT^TAAT
AsnI 1042 ATTAAT AT^TAAT
AspHI 810 GAGCAC G[AT]GC[AT]^C
BanI 888 GGCGCC G^G[CT][GA]CC
BbeI 888 GGCGCC GGCGC^C
BbvI 345 GCTGC GCAGC(8/12)
BclI 47 TGATCA T^GATCA
BsaHI 888 GGCGCC G[GA]^CG[CT]C
BsaWI 380 TCCGGA [AT]^CCGG[AT]
BseAI 380 TCCGGA T^CCGGA
BseRI 373 GAGGAG GAGGAG(10/8)
BsiEI 1228 CGATCG CG[GA][CT]^CG
BsiHKAI 810 GAGCAC G[AT]GC[AT]^C
BsmBI 992 CGTCTC CGTCTC(1/5)
Bsp1407I 638 TGTACA T^GTACA
BspEI 380 TCCGGA T^CCGGA
BspHI 407 TCATGA T^CATGA
BsrBI 1247 GAGCGG CCGCTC(-3/-3)
BsrGI 638 TGTACA T^GTACA
BstEII 828 GGTGACC G^GT[ACGT]ACC
BstPI 828 GGTGACC G^GT[ACGT]ACC
BstUI 817 CGCG CG^CG
BstYI 851 AGATCC [GA]^GATC[CT]
Bsu36I 1111 CCTAAGG CC^T[ACGT]AGG
DrdI 175 GACCAAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 384 GAAGAG CTCTTC(1/4)
Eco81I 1111 CCTAAGG CC^T[ACGT]AGG
EcoO65I 828 GGTGACC G^GT[ACGT]ACC
EcoT14I 765 CCATGG C^C[AT][AT]GG
FbaI 47 TGATCA T^GATCA
HaeII 888 GGCGCC [GA]GCGC^[CT]
HgaI 985 GCGTC GACGC(5/10)
Hin1I 888 GGCGCC G[GA]^CG[CT]C
HincII 184 GTCAAC GT[CT]^[GA]AC
HindII 184 GTCAAC GT[CT]^[GA]AC
HindIII 643 AAGCTT A^AGCTT
KasI 888 GGCGCC G^GCGCC
Ksp632I 384 GAAGAG CTCTTC(1/4)
KspI 816 CCGCGG CCGC^GG
MflI 851 AGATCC [GA]^GATC[CT]
MroI 380 TCCGGA T^CCGGA
MvnI 817 CGCG CG^CG
NarI 888 GGCGCC GG^CGCC
NcoI 765 CCATGG C^CATGG
PflMI 395 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 221 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1042 ATTAAT AT^TAAT
PvuI 1228 CGATCG CGAT^CG
RcaI 407 TCATGA T^CATGA
SacII 816 CCGCGG CCGC^GG
SapI 384 GAAGAGC GCTCTTC(1/4)
SfaNI 440 GCATC GCATC(5/9)
SfoI 888 GGCGCC GGC^GCC
SgrAI 890 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 482 CCCGGG CCC^GGG
SmlI 1027 CTCAAG C^T[CT][GA]AG
SspBI 638 TGTACA T^GTACA
StyI 765 CCATGG C^C[AT][AT]GG
TseI 345 GCTGC G^C[AT]GC
Van91I 395 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1084 CCAGACTGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 851 AGATCC [GA]^GATC[CT]
XmaCI 482 CCCGGG C^CCGGG
XmaI 482 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 635 ACATGT A^C[GA][CT]GT
704 ACATGT
BanII 387 GAGCCC G[GA]GC[CT]^C
1213 GGGCCC
BpmI 262 CTGGAG CTGGAG(16/14)
805 CTGGAG
BsaAI 532 TACGTG [CT]AC^GT[GA]
612 CACGTA
BsgI 663 CTGCAC GTGCAG(16/14)
868 GTGCAG
BsmI 922 GAATGC GAATGC(1/-1)
981 GAATGC
BspLU11I 635 ACATGT A^CATGT
704 ACATGT
BspMI 587 ACCTGC ACCTGC(4/8)
791 ACCTGC
BsrFI 656 GCCGGT [GA]^CCGG[CT]
891 GCCGGT
BstXI 689 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
759 CCACATCCATGG
Cfr10I 656 GCCGGT [GA]^CCGG[CT]
891 GCCGGT
DraII 221 AGGACCT [GA]G^G[ACGT]CC[CT]
1212 GGGGCCC
DsaI 765 CCATGG C^C[GA][CT]GG
816 CCGCGG
EcoO109I 221 AGGACCT [GA]G^G[ACGT]CC[CT]
1212 GGGGCCC
EcoRV 28 GATATC GAT^ATC
158 GATATC
MseI 551 TTAA T^TAA
1043 TTAA
MslI 336 CACCTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
760 CACATCCATG
MspA1I 350 CCGCTG C[AC]G^C[GT]G
816 CCGCGG
NspI 635 ACATGT [GA]CATG^[CT]
704 ACATGT
PleI 1237 GACTC GAGTC(4/5)
276 GAGTC
TfiI 1022 GAATC G^A[AT]TC
1137 GATTC
Tru9I 551 TTAA T^TAA
1043 TTAA
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BcgI BcnI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TaqI
Tsp45I Tsp509I TspRI TthHB8I