Restriction map for
pF1KE0307
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BamHI BbeI
BciVI BfrI BglII BlnI BseAI
BsgI BsiEI BsiWI BsoBI Bsp1407I
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Cfr10I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI HpaI KasI KpnI KspI
MfeI MflI MluI MroI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RsrII SacII ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 2047 GTCGAC GT^[AC][GT]AC
AccII 2015 CGCG CG^CG
Alw44I 151 GTGCAC G^TGCAC
AlwNI 919 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1681 CCTCAGG CC^T[ACGT]AGG
ApaI 2005 GGGCCC GGGCC^C
ApaLI 151 GTGCAC G^TGCAC
AvaII 591 GGACC G^G[AT]CC
AviII 1903 TGCGCA TGC^GCA
BanI 1932 GGTGCC G^G[CT][GA]CC
BbrPI 707 CACGTG CAC^GTG
BbsI 190 GAAGAC GAAGAC(2/6)
BbvCI 1997 CCTCAGC CCTCAGC(-5/-2)
BcgI 1134 CGAGGTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1797 TGATCA T^GATCA
BfaI 1318 CTAG C^TAG
BlpI 1072 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1072 GCTCAGC GC^T[ACGT]AGC
BpuAI 190 GAAGAC GAAGAC(2/6)
BsaAI 707 CACGTG [CT]AC^GT[GA]
BsaI 1774 GGTCTC GGTCTC(1/5)
BsmI 270 GAATGC GAATGC(1/-1)
BspDI 856 ATCGAT AT^CGAT
BspHI 1520 TCATGA T^CATGA
BspMI 437 ACCTGC ACCTGC(4/8)
BsrBI 1607 CCGCTC CCGCTC(-3/-3)
BssSI 1964 CACGAG CACGAG(-5/-1)
BstUI 2015 CGCG CG^CG
Bsu36I 1681 CCTCAGG CC^T[ACGT]AGG
CelII 1072 GCTCAGC GC^T[ACGT]AGC
ClaI 856 ATCGAT AT^CGAT
DraI 898 TTTAAA TTT^AAA
DsaI 1928 CCATGG C^C[GA][CT]GG
Eco81I 1681 CCTCAGG CC^T[ACGT]AGG
EcoNI 87 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1105 GAATTC G^AATTC
EcoRV 160 GATATC GAT^ATC
FbaI 1797 TGATCA T^GATCA
FspI 1903 TGCGCA TGC^GCA
HaeII 693 GGCGCT [GA]GCGC^[CT]
MaeI 1318 CTAG C^TAG
MvnI 2015 CGCG CG^CG
NcoI 1928 CCATGG C^CATGG
PmaCI 707 CACGTG CAC^GTG
PmlI 707 CACGTG CAC^GTG
PshAI 1402 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1520 TCATGA T^CATGA
SacI 868 GAGCTC GAGCT^C
SalI 2047 GTCGAC G^TCGAC
SbfI 1065 CCTGCAGG CCTGCA^GG
Sse8387I 1065 CCTGCAGG CCTGCA^GG
StuI 1859 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 575 AAATTC [GA]^AATT[CT]
1105 GAATTC
AlwI 1638 GATCC GGATC(4/5)
1452 GGATC
ApoI 575 AAATTC [GA]^AATT[CT]
1105 GAATTC
BalI 102 TGGCCA TGG^CCA
1508 TGGCCA
BcnI 654 CCGGG CC^[GC]GG
1745 CCGGG
BglI 1840 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1947 GCCCAGTAGGC
BsaBI 520 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1302 GATGAAAATC
BsaWI 471 ACCGGA [AT]^CCGG[AT]
1831 TCCGGT
BsmBI 1443 CGTCTC CGTCTC(1/5)
1376 GAGACG
BsmFI 299 GGGAC GGGAC(10/14)
1661 GTCCC
EaeI 102 TGGCCA [CT]^GGCC[GA]
1508 TGGCCA
EcoT14I 1724 CCAAGG C^C[AT][AT]GG
1928 CCATGG
FauI 1548 CCCGC CCCGC(4/6)
702 GCGGG
HindIII 937 AAGCTT A^AGCTT
1826 AAGCTT
MamI 520 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1302 GATGAAAATC
MluNI 102 TGGCCA TGG^CCA
1508 TGGCCA
MscI 102 TGGCCA TGG^CCA
1508 TGGCCA
NciI 654 CCGGG CC^[GC]GG
1745 CCGGG
NspI 1900 GCATGC [GA]CATG^[CT]
1939 GCATGC
PvuII 184 CAGCTG CAG^CTG
2000 CAGCTG
SmlI 940 CTTGAG C^T[CT][GA]AG
1612 CTCAAG
SphI 1900 GCATGC GCATG^C
1939 GCATGC
SspI 894 AATATT AAT^ATT
998 AATATT
StyI 1724 CCAAGG C^C[AT][AT]GG
1928 CCATGG
Tsp45I 56 GTGAC ^GT[GC]AC
77 GTCAC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
BanII BbvI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I