Restriction map for
pF1KE0549
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TseI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 403 AAATTC [GA]^AATT[CT]
AfaI 180 GTAC GT^AC
ApoI 403 AAATTC [GA]^AATT[CT]
Asp700I 57 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 352 GAGCAC G[AT]GC[AT]^C
AvaII 393 GGACC G^G[AT]CC
BcgI 354 GCACCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 373 CTAG C^TAG
BglII 218 AGATCT A^GATCT
BmyI 352 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 352 GAGCAC G[AT]GC[AT]^C
BsmFI 318 GTCCC GGGAC(10/14)
BsmI 414 GAATGC GAATGC(1/-1)
Bsp1286I 352 GAGCAC G[AGT]GC[ACT]^C
BspMI 249 GCAGGT ACCTGC(4/8)
BssKI 75 CCAGG ^CC[ACGT]GG
BstNI 75 CCAGG CC^[AT]GG
BstYI 218 AGATCT [GA]^GATC[CT]
Cfr13I 393 GGACC G^G[ACGT]CC
DraI 208 TTTAAA TTT^AAA
EaeI 5 CGGCCA [CT]^GGCC[GA]
EarI 421 GAAGAG CTCTTC(1/4)
EcoRII 75 CCAGG ^CC[AT]GG
FauI 357 CCCGC CCCGC(4/6)
Fnu4HI 4 GCGGC GC^[ACGT]GC
HaeIII 6 GGCC GG^CC
HinfI 155 GAATC G^A[ACGT]TC
ItaI 4 GCGGC GC^[ACGT]GC
Ksp632I 421 GAAGAG CTCTTC(1/4)
MaeI 373 CTAG C^TAG
MaeIII 68 GTAAC ^GT[ACGT]AC
MflI 218 AGATCT [GA]^GATC[CT]
MseI 209 TTAA T^TAA
MvaI 75 CCAGG CC^[AT]GG
MwoI 367 GCTGACCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 134 CATATG CA^TATG
NlaIV 145 GGAGCC GG[ACGT]^[ACGT]CC
RsaI 180 GTAC GT^AC
Sau96I 393 GGACC G^G[ACGT]CC
ScrFI 75 CCAGG CC^[ACGT]GG
TfiI 155 GAATC G^A[AT]TC
Tru9I 209 TTAA T^TAA
TspRI 329 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 218 AGATCT [GA]^GATC[CT]
XmnI 57 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BstF5I 72 CATCC GGATG(2/0)
53 GGATG
Cac8I 7 GCCAGC GC[ACGT]^[ACGT]GC
288 GCCAGC
DdeI 350 CTGAG C^T[ACGT]AG
389 CTAAG
DpnII 201 GATC ^GATC
219 GATC
FokI 72 CATCC GGATG(9/13)
53 GGATG
HphI 333 GGTGA GGTGA(8/7)
225 TCACC
MboI 201 GATC ^GATC
219 GATC
NdeII 201 GATC ^GATC
219 GATC
Sau3AI 201 GATC ^GATC
219 GATC
TaqI 158 TCGA T^CGA
236 TCGA
TthHB8I 158 TCGA T^CGA
236 TCGA
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AciI AluI BsiYI BslI BsrI
MboII MnlI Tsp509I