Restriction map for
pF1KE0550
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI SacI SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 624 GTCGAC GT^[AC][GT]AC
AccII 676 CGCG CG^CG
AfaI 66 GTAC GT^AC
AflIII 14 ACATGT A^C[GA][CT]GT
AlwI 688 GGATC GGATC(4/5)
BalI 356 TGGCCA TGG^CCA
BbsI 40 GTCTTC GAAGAC(2/6)
BbvI 71 GCTGC GCAGC(8/12)
BcgI 863 GCAGATTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 461 CCGGG CC^[GC]GG
BfaI 91 CTAG C^TAG
BglII 494 AGATCT A^GATCT
BpuAI 40 GTCTTC GAAGAC(2/6)
BsaBI 471 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 517 GAGACC GGTCTC(1/5)
BseRI 619 GAGGAG GAGGAG(10/8)
BsmAI 517 GAGAC GTCTC(1/5)
BsmFI 824 GTCCC GGGAC(10/14)
BspLU11I 14 ACATGT A^CATGT
BsrDI 896 CATTGC GCAATG(2/0)
BstUI 676 CGCG CG^CG
CfoI 677 GCGC GCG^C
CpoI 221 CGGACCG CG^G[AT]CCG
DraI 787 TTTAAA TTT^AAA
DraII 96 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 356 TGGCCA [CT]^GGCC[GA]
EarI 844 GAAGAG CTCTTC(1/4)
EcoNI 324 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 96 AGGGCCT [GA]G^G[ACGT]CC[CT]
HapII 461 CCGG C^CGG
HhaI 677 GCGC GCG^C
HinP1I 677 GCGC G^CGC
HpaII 461 CCGG C^CGG
Ksp632I 844 GAAGAG CTCTTC(1/4)
MaeI 91 CTAG C^TAG
MaeIII 554 GTGAC ^GT[ACGT]AC
MamI 471 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
MslI 558 CATGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 69 CCGCTG C[AC]G^C[GT]G
MspI 461 CCGG C^CGG
MvnI 676 CGCG CG^CG
MwoI 90 GCTAGCAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 461 CCGGG CC^[GC]GG
NheI 90 GCTAGC G^CTAGC
NspI 14 ACATGT [GA]CATG^[CT]
PleI 622 GAGTC GAGTC(4/5)
RsaI 66 GTAC GT^AC
RsrII 221 CGGACCG CG^G[AT]CCG
SalI 624 GTCGAC G^TCGAC
TfiI 866 GATTC G^A[AT]TC
TseI 71 GCTGC G^C[AT]GC
Tsp45I 554 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AluI 665 AGCT AG^CT
855 AGCT
BanII 384 GAGCCC G[GA]GC[CT]^C
727 GAGCCC
BmyI 384 GAGCCC G[AGT]GC[ACT]^C
727 GAGCCC
Bsp1286I 384 GAGCCC G[AGT]GC[ACT]^C
727 GAGCCC
BsrI 287 ACTGG ACTGG(1/-1)
702 ACTGG
BstYI 494 AGATCT [GA]^GATC[CT]
688 GGATCT
Cac8I 90 GCTAGC GC[ACGT]^[ACGT]GC
242 GCGTGC
DpnII 495 GATC ^GATC
689 GATC
EcoRI 216 GAATTC G^AATTC
770 GAATTC
Fnu4HI 71 GCTGC GC^[ACGT]GC
320 GCGGC
HgaI 231 GACGC GACGC(5/10)
341 GCGTC
HincII 343 GTCAAC GT[CT]^[GA]AC
624 GTCGAC
HindII 343 GTCAAC GT[CT]^[GA]AC
624 GTCGAC
HinfI 622 GAGTC G^A[ACGT]TC
866 GATTC
HphI 634 GGTGA GGTGA(8/7)
404 TCACC
ItaI 71 GCTGC GC^[ACGT]GC
320 GCGGC
MaeII 563 ACGT A^CGT
822 ACGT
MboI 495 GATC ^GATC
689 GATC
MflI 494 AGATCT [GA]^GATC[CT]
688 GGATCT
NdeII 495 GATC ^GATC
689 GATC
Sau3AI 495 GATC ^GATC
689 GATC
TaqI 20 TCGA T^CGA
625 TCGA
TthHB8I 20 TCGA T^CGA
625 TCGA
XhoII 494 AGATCT [GA]^GATC[CT]
688 GGATCT
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AcsI ApoI AvaII BpmI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cfr13I DdeI EcoRII FokI
HaeIII MboII MnlI MseI MvaI
NlaIII Sau96I ScrFI SfaNI Tru9I
Tsp509I TspRI