Restriction map for
pF1KE0362
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI AhdI AlwNI Aor51HI ApaI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI Bst1107I BstAPI BstBI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO109I EcoRI EcoRV EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI Ksp632I KspI MaeI
MamI MflI MluI MluNI MroI
MscI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1439 GGTACC G^GTACC
AccI 44 GTCTAC GT^[AC][GT]AC
AccII 1401 CGCG CG^CG
AcyI 595 GACGCC G[GA]^CG[CT]C
Alw44I 539 GTGCAC G^TGCAC
AocI 147 CCTCAGG CC^T[ACGT]AGG
ApaLI 539 GTGCAC G^TGCAC
AscI 1399 GGCGCGCC GG^CGCGCC
AseI 871 ATTAAT AT^TAAT
AsnI 871 ATTAAT AT^TAAT
Asp718I 1439 GGTACC G^GTACC
AspHI 539 GTGCAC G[AT]GC[AT]^C
BciVI 780 GTATCC GTATCC(6/5)
BclI 1176 TGATCA T^GATCA
BsaHI 595 GACGCC G[GA]^CG[CT]C
BsaI 921 GGTCTC GGTCTC(1/5)
BsaWI 1532 ACCGGA [AT]^CCGG[AT]
BseRI 554 CTCCTC GAGGAG(10/8)
BsgI 8 GTGCAG GTGCAG(16/14)
BsiHKAI 539 GTGCAC G[AT]GC[AT]^C
BsmFI 568 GTCCC GGGAC(10/14)
BspMI 1409 ACCTGC ACCTGC(4/8)
BssHII 1400 GCGCGC G^CGCGC
BssSI 5 CTCGTG CACGAG(-5/-1)
BstEII 1075 GGTCACC G^GT[ACGT]ACC
BstPI 1075 GGTCACC G^GT[ACGT]ACC
BstUI 1401 CGCG CG^CG
Bsu36I 147 CCTCAGG CC^T[ACGT]AGG
DraI 1510 TTTAAA TTT^AAA
Eco81I 147 CCTCAGG CC^T[ACGT]AGG
EcoNI 1326 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1075 GGTCACC G^GT[ACGT]ACC
FauI 1417 CCCGC CCCGC(4/6)
FbaI 1176 TGATCA T^GATCA
HgaI 595 GACGC GACGC(5/10)
Hin1I 595 GACGCC G[GA]^CG[CT]C
HinfI 1020 GAATC G^A[ACGT]TC
KpnI 1439 GGTACC GGTAC^C
MaeII 1460 ACGT A^CGT
MfeI 953 CAATTG C^AATTG
MslI 1152 CATGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1351 CCGCTG C[AC]G^C[GT]G
MunI 953 CAATTG C^AATTG
MvnI 1401 CGCG CG^CG
PflMI 1449 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 871 ATTAAT AT^TAAT
SexAI 1435 ACCTGGT A^CC[AT]GGT
SfcI 732 CTACAG C^T[GA][CT]AG
TfiI 1020 GAATC G^A[AT]TC
Van91I 1449 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanI 297 GGCACC G^G[CT][GA]CC
1439 GGTACC
BbsI 222 GAAGAC GAAGAC(2/6)
499 GTCTTC
BbvI 1420 GCAGC GCAGC(8/12)
81 GCTGC
BcnI 261 CCGGG CC^[GC]GG
1341 CCCGG
BpuAI 222 GAAGAC GAAGAC(2/6)
499 GTCTTC
BsmAI 1204 GAGAC GTCTC(1/5)
922 GTCTC
BsrDI 302 CATTGC GCAATG(2/0)
1139 GCAATG
Cac8I 286 GCTCGC GC[ACGT]^[ACGT]GC
1400 GCGCGC
EaeI 1361 TGGCCG [CT]^GGCC[GA]
1478 TGGCCG
NciI 261 CCGGG CC^[GC]GG
1341 CCCGG
NdeI 722 CATATG CA^TATG
1134 CATATG
SspI 476 AATATT AAT^ATT
513 AATATT
TaqI 440 TCGA T^CGA
1018 TCGA
TseI 81 GCTGC G^C[AT]GC
1420 GCAGC
TthHB8I 440 TCGA T^CGA
1018 TCGA
XcmI 119 CCATGCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
178 CCAGAGGTGAGTTGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI CfoI Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI Tru9I Tsp45I Tsp509I TspRI