################################## # NO CUT ENZYMES ( 198 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DpnII DraII DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI TaqI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### BfaI 206 CTAG C^TAG BsaAI 299 CACGTA [CT]AC^GT[GA] BsaJI 23 CCCCGG C^C[ACGT][ACGT]GG BsiEI 26 CGGCCG CG[GA][CT]^CG BsmFI 21 GTCCC GGGAC(10/14) Bsp1407I 285 TGTACA T^GTACA BsrGI 285 TGTACA T^GTACA BstNI 33 CCAGG CC^[AT]GG Cac8I 4 GCTTGC GC[ACGT]^[ACGT]GC DraI 66 TTTAAA TTT^AAA EaeI 26 CGGCCG [CT]^GGCC[GA] EagI 26 CGGCCG C^GGCCG EclXI 26 CGGCCG C^GGCCG Eco52I 26 CGGCCG C^GGCCG EcoRII 33 CCAGG ^CC[AT]GG HaeIII 27 GGCC GG^CC MaeI 206 CTAG C^TAG MvaI 33 CCAGG CC^[AT]GG MwoI 4 GCTTGCGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PleI 242 GAGTC GAGTC(4/5) PshAI 376 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SmlI 184 CTTGAG C^T[CT][GA]AG SspBI 285 TGTACA T^GTACA SwaI 65 ATTTAAAT ATTT^AAAT XbaI 205 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 15 CGCG CG^CG 17 CGCG AciI 14 CCGC CCGC(-3/-1) 18 GCGG AfaI 201 GTAC GT^AC 286 GTAC AluI 135 AGCT AG^CT 254 AGCT AvaII 20 GGTCC G^G[AT]CC 249 GGTCC BbsI 78 GAAGAC GAAGAC(2/6) 383 GTCTTC BbvI 322 GCAGC GCAGC(8/12) 10 GCTGC BcnI 24 CCCGG CC^[GC]GG 246 CCGGG BpuAI 78 GAAGAC GAAGAC(2/6) 383 GTCTTC BstUI 15 CGCG CG^CG 17 CGCG CfoI 8 GCGC GCG^C 16 GCGC Cfr13I 20 GGTCC G^G[ACGT]CC 249 GGTCC HapII 25 CCGG C^CGG 246 CCGG HhaI 8 GCGC GCG^C 16 GCGC HinP1I 8 GCGC G^CGC 16 GCGC HpaII 25 CCGG C^CGG 246 CCGG MaeII 176 ACGT A^CGT 300 ACGT MseI 67 TTAA T^TAA 173 TTAA MspI 25 CCGG C^CGG 246 CCGG MvnI 15 CGCG CG^CG 17 CGCG NciI 24 CCCGG CC^[GC]GG 246 CCGGG RsaI 201 GTAC GT^AC 286 GTAC Sau96I 20 GGTCC G^G[ACGT]CC 249 GGTCC SfcI 131 CTACAG C^T[GA][CT]AG 137 CTACAG Tru9I 67 TTAA T^TAA 173 TTAA TseI 10 GCTGC G^C[AT]GC 322 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### BssKI Fnu4HI HinfI ItaI MboII MnlI NlaIV ScrFI TfiI