Restriction map for
pF1KE0551
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccII AccIII AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI HindIII
HpaI KpnI Ksp632I KspI MaeI
MfeI MluI MroI MseI MspA1I
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI PvuII RcaI RsrII
SacI SacII SapI SbfI ScaI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 261 AACGTT AA^CGTT
AcyI 682 GGCGCC G[GA]^CG[CT]C
AfaI 483 GTAC GT^AC
ApaI 14 GGGCCC GGGCC^C
BamHI 432 GGATCC G^GATCC
BbeI 682 GGCGCC GGCGC^C
BbrPI 594 CACGTG CAC^GTG
BbsI 789 GTCTTC GAAGAC(2/6)
BcgI 460 GCACCCCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 810 CCGGG CC^[GC]GG
BpmI 853 CTCCAG CTGGAG(16/14)
BpuAI 789 GTCTTC GAAGAC(2/6)
BsaAI 594 CACGTG [CT]AC^GT[GA]
BsaBI 624 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 682 GGCGCC G[GA]^CG[CT]C
BsiEI 587 CGATCG CG[GA][CT]^CG
BsrDI 687 CATTGC GCAATG(2/0)
BsrFI 605 GCCGGC [GA]^CCGG[CT]
CfoI 683 GCGC GCG^C
Cfr10I 605 GCCGGC [GA]^CCGG[CT]
DrdI 537 GACCCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 682 GGCGCC [GA]GCGC^[CT]
HhaI 683 GCGC GCG^C
Hin1I 682 GGCGCC G[GA]^CG[CT]C
HinP1I 683 GCGC G^CGC
HincII 199 GTCGAC GT[CT]^[GA]AC
HindII 199 GTCGAC GT[CT]^[GA]AC
HphI 572 TCACC GGTGA(8/7)
KasI 682 GGCGCC G^GCGCC
MamI 624 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 790 TCTTC GAAGA(8/7)
MslI 798 CACGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 605 GCCGGC GCC^GGC
NarI 682 GGCGCC GG^CGCC
NciI 810 CCGGG CC^[GC]GG
NgoMIV 605 GCCGGC G^CCGGC
NspI 140 GCATGT [GA]CATG^[CT]
PmaCI 594 CACGTG CAC^GTG
PmlI 594 CACGTG CAC^GTG
Psp1406I 261 AACGTT AA^CGTT
PvuI 587 CGATCG CGAT^CG
RsaI 483 GTAC GT^AC
SalI 199 GTCGAC G^TCGAC
SexAI 395 ACCTGGT A^CC[AT]GGT
SfaNI 349 GATGC GCATC(5/9)
SfiI 15 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 682 GGCGCC GGC^GCC
StuI 371 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 181 GTCTAC GT^[AC][GT]AC
199 GTCGAC
AflIII 593 ACACGT A^C[GA][CT]GT
797 ACACGT
AlwNI 379 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
535 CAGACCCTG
BalI 74 TGGCCA TGG^CCA
598 TGGCCA
BbvCI 693 CCTCAGC CCTCAGC(-5/-2)
832 GCTGAGG
BglI 16 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
600 GCCAGGCCGGC
BsmFI 418 GGGAC GGGAC(10/14)
293 GTCCC
BsmI 219 GAATGC GAATGC(1/-1)
387 GAATGC
BsrBI 7 CCGCTC CCGCTC(-3/-3)
612 GAGCGG
BssSI 175 CACGAG CACGAG(-5/-1)
700 CTCGTG
BstXI 515 CCACGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
767 CCAAGTCGCTGG
BstYI 380 AGATCC [GA]^GATC[CT]
432 GGATCC
DsaI 476 CCACGG C^C[GA][CT]GG
515 CCACGG
EaeI 74 TGGCCA [CT]^GGCC[GA]
598 TGGCCA
EcoT14I 122 CCTTGG C^C[AT][AT]GG
368 CCAAGG
HapII 606 CCGG C^CGG
810 CCGG
HpaII 606 CCGG C^CGG
810 CCGG
MflI 380 AGATCC [GA]^GATC[CT]
432 GGATCC
MluNI 74 TGGCCA TGG^CCA
598 TGGCCA
MscI 74 TGGCCA TGG^CCA
598 TGGCCA
MspI 606 CCGG C^CGG
810 CCGG
PpuMI 291 AGGTCCC [GA]G^G[AT]CC[CT]
418 GGGACCT
PstI 61 CTGCAG CTGCA^G
327 CTGCAG
SfcI 61 CTGCAG C^T[GA][CT]AG
327 CTGCAG
StyI 122 CCTTGG C^C[AT][AT]GG
368 CCAAGG
Tsp45I 82 GTGAC ^GT[GC]AC
332 GTGAC
XcmI 113 CCACTGTGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
242 CCAACACCCCACTGG
XhoII 380 AGATCC [GA]^GATC[CT]
432 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BanII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeII
MaeIII MboI MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TseI TspRI
TthHB8I