Restriction map for
pF1KA1594
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AvaI BalI BanI
BbeI BbrPI BclI BfrI BglI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsmFI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstZ17I CelII CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I FauI
FbaI FseI HaeII Hin1I HindIII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 210 CCGC CCGC(-3/-1)
AclI 1970 AACGTT AA^CGTT
AhdI 2919 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 658 CCTAAGG CC^T[ACGT]AGG
Asp700I 2116 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2919 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1764 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1821 TGCGCA TGC^GCA
AvrII 1666 CCTAGG C^CTAGG
BanII 1923 GAGCTC G[GA]GC[CT]^C
BcgI 2805 GCAGAGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1118 GTATCC GTATCC(6/5)
BcnI 496 CCGGG CC^[GC]GG
BglII 2855 AGATCT A^GATCT
BlnI 1666 CCTAGG C^CTAGG
BsaBI 2812 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1201 GAGACC GGTCTC(1/5)
BsaWI 2212 ACCGGA [AT]^CCGG[AT]
BsgI 2803 GTGCAG GTGCAG(16/14)
BsmBI 338 CGTCTC CGTCTC(1/5)
BsmI 1056 GAATGC GAATGC(1/-1)
BspDI 507 ATCGAT AT^CGAT
BspMI 1045 ACCTGC ACCTGC(4/8)
BsrDI 1777 CATTGC GCAATG(2/0)
BsrFI 2666 ACCGGC [GA]^CCGG[CT]
BstAPI 1011 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 658 CCTAAGG CC^T[ACGT]AGG
CfoI 1822 GCGC GCG^C
Cfr10I 2666 ACCGGC [GA]^CCGG[CT]
ClaI 507 ATCGAT AT^CGAT
DsaI 1123 CCATGG C^C[GA][CT]GG
Eam1105I 2919 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 658 CCTAAGG CC^T[ACGT]AGG
EcoT22I 2549 ATGCAT ATGCA^T
FspI 1821 TGCGCA TGC^GCA
HgaI 1157 GACGC GACGC(5/10)
HhaI 1822 GCGC GCG^C
HinP1I 1822 GCGC G^CGC
HincII 219 GTTAAC GT[CT]^[GA]AC
HindII 219 GTTAAC GT[CT]^[GA]AC
HpaI 219 GTTAAC GTT^AAC
MaeII 1971 ACGT A^CGT
MamI 2812 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 496 CCGGG CC^[GC]GG
NcoI 1123 CCATGG C^CATGG
NdeI 1825 CATATG CA^TATG
NsiI 2549 ATGCAT ATGCA^T
NspI 1471 GCATGT [GA]CATG^[CT]
PflFI 1764 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1970 AACGTT AA^CGTT
SacI 1923 GAGCTC GAGCT^C
SmlI 1219 CTCAAG C^T[CT][GA]AG
StuI 2930 AGGCCT AGG^CCT
Tth111I 1764 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2116 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AspHI 1634 GTGCTC G[AT]GC[AT]^C
1923 GAGCTC
BseRI 5 CTCCTC GAGGAG(10/8)
1550 CTCCTC
BsiHKAI 1634 GTGCTC G[AT]GC[AT]^C
1923 GAGCTC
BspHI 1287 TCATGA T^CATGA
2175 TCATGA
BstBI 35 TTCGAA TT^CGAA
476 TTCGAA
DraII 379 AGGACCT [GA]G^G[ACGT]CC[CT]
1670 GGGTCCT
EcoO109I 379 AGGACCT [GA]G^G[ACGT]CC[CT]
1670 GGGTCCT
EcoRV 624 GATATC GAT^ATC
1186 GATATC
MslI 2176 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2904 CACGGAAGTG
NspV 35 TTCGAA TT^CGAA
476 TTCGAA
PpuMI 379 AGGACCT [GA]G^G[AT]CC[CT]
1670 GGGTCCT
PstI 323 CTGCAG CTGCA^G
1018 CTGCAG
RcaI 1287 TCATGA T^CATGA
2175 TCATGA
SapI 2116 GAAGAGC GCTCTTC(1/4)
2419 GAAGAGC
SfuI 35 TTCGAA TT^CGAA
476 TTCGAA
Tsp45I 2687 GTCAC ^GT[GC]AC
2895 GTCAC
XbaI 294 TCTAGA T^CTAGA
1837 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BamHI BbsI BbvCI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII