##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaWI       BseAI       BseRI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AlwNI         178  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI          157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BclI          293  TGATCA               T^GATCA
BcnI           42  CCCGG                CC^[GC]GG
BsaI          173  GAGACC               GGTCTC(1/5)
BsiEI         198  CGACCG               CG[GA][CT]^CG
BsmFI          59  GTCCC                GGGAC(10/14)
BspHI         140  TCATGA               T^CATGA
DpnII         294  GATC                 ^GATC
DsaI           23  CCACGG               C^C[GA][CT]GG
FauI          220  GCGGG                CCCGC(4/6)
FbaI          293  TGATCA               T^GATCA
HapII          43  CCGG                 C^CGG
HpaII          43  CCGG                 C^CGG
MaeII         152  ACGT                 A^CGT
MaeIII         89  GTAAC                ^GT[ACGT]AC
MboI          294  GATC                 ^GATC
MslI          141  CATGAGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         17  CCGCTG               C[AC]G^C[GT]G
MspI           43  CCGG                 C^CGG
NciI           42  CCCGG                CC^[GC]GG
NdeII         294  GATC                 ^GATC
NlaIII        141  CATG                 CATG^
PflFI         157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         161  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          100  CTGCAG               CTGCA^G
RcaI          140  TCATGA               T^CATGA
Sau3AI        294  GATC                 ^GATC
SfaNI         208  GATGC                GCATC(5/9)
SfcI          100  CTGCAG               C^T[GA][CT]AG
Tth111I       157  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           17  CCGC                 CCGC(-3/-1)
              220  GCGG                
AvaII          58  GGTCC                G^G[AT]CC
              162  GGTCC               
BpmI          214  CTCCAG               CTGGAG(16/14)
              166  CTGGAG              
BsaBI         288  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              291  GATGATCATC          
BsgI           65  CTGCAC               GTGCAG(16/14)
              203  GTGCAG              
BsmAI          96  GAGAC                GTCTC(1/5)
              173  GAGAC               
BstF5I        321  CATCC                GGATG(2/0)
              123  GGATG               
DraII         161  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              223  GGGGCCT             
EcoO109I      161  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              223  GGGGCCT             
FokI          321  CATCC                GGATG(9/13)
              123  GGATG               
HphI          147  GGTGA                GGTGA(8/7)
               83  TCACC               
MamI          288  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              291  GATGATCATC          
SmlI          256  CTCAAG               C^T[CT][GA]AG
              283  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstNI       CfoI        Cfr13I      
EcoRII      Fnu4HI      HaeIII      HhaI        HinP1I      
ItaI        MnlI        MvaI        MwoI        NlaIV       
Sau96I      ScrFI       TseI